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62 条记录 (耗时 0.033 秒)

    Carrier Peptide Fragment and Use Thereof
    52.
    发明申请
    Carrier Peptide Fragment and Use Thereof 有权

    公开(公告)号：US20220153784A1

    公开(公告)日：2022-05-19

    申请号：US17525097

    申请日：2021-11-12

    申请人： TOAGOSEI CO., LTD.

    发明人： Nahoko Baileykobayashi ,  Tetsuhiko Yoshida

    IPC分类号： C07K7/08 ,  C07K14/00 ,  C12N5/09

    摘要： The present disclosure provides an artificially synthesized peptide fragment that can efficiently introduce a foreign substance of interest from outside a eukaryotic cell into at least the cytoplasm of the cell. The present disclosure also provides a method for using the peptide fragment. The herein disclosed peptide fragment is a peptide fragment for introducing a foreign substance of interest from outside a eukaryotic cell into at least the cytoplasm of the cell, and is provided with a repeat sequence in which the VVRSLVK amino acid sequence (SEQ ID NO: 1) is repeated at least two or more times. By using a construct that has the peptide fragment and the foreign substance of interest bonded to the N-terminal side and/or C-terminal side of the peptide fragment, the foreign substance of interest can be efficiently introduced from outside a eukaryotic cell into at least the cytoplasm of the cell.

    Carrier Peptide Fragment and Use Thereof
    53.
    发明申请
    Carrier Peptide Fragment and Use Thereof 有权

    公开(公告)号：US20220089641A1

    公开(公告)日：2022-03-24

    申请号：US17472310

    申请日：2021-09-10

    申请人： TOAGOSEI CO., LTD.

    发明人： Nahoko Baileykobayashi ,  Tetsuhiko Yoshida

    IPC分类号： C07K7/08 ,  C12N5/09

    摘要： The disclosure provides a technology for efficiently introducing a foreign substance into at least cytoplasm of eukaryotic cells from outside the cells. The method disclosed here for introducing a foreign substance of interest in vitro or in vivo into at least cytoplasm of eukaryotic cells from outside the cells includes (1) a step of preparing a foreign substance introduction construct including a carrier fragment composed of KKRTLRKSNRKKR (SEQ ID NO:1) and the foreign substance of interest linked to the N-terminal and/or C-terminal side of the carrier peptide fragment; (2) a step of supplying the foreign substance introduction construct to a sample containing a target eukaryotic cell; and (3) a step of incubating the sample, to which the foreign substance introduction construct has been supplied, to thereby introduce the construct into the eukaryotic cell in the sample.

    EXOSOME PRODUCTION METHOD
    54.
    发明申请
    EXOSOME PRODUCTION METHOD 审中-公开

    公开(公告)号：US20200231647A1

    公开(公告)日：2020-07-23

    申请号：US16743379

    申请日：2020-01-15

    申请人： TOAGOSEI CO., LTD. ,  University Public Corporation Osaka

    发明人： Nahoko Baileykobayashi ,  Tetsuhiko Yoshida ,  Ikuhiko Nakase

    IPC分类号： C07K14/705 ,  C12N5/09

    摘要： The method of producing induced lightweight exosomes (iLE) provided by the present invention includes preparing a cell culture containing prescribed cultured cells; supplying at least once an artificially produced synthetic peptide to the cell culture and culturing the cell culture for a prescribed interval; and obtaining exosomes produced by the cultured cells following culturing. Here, the synthetic peptide is provided with: (1) an exosome-inducing peptide sequence and (2) a CPP sequence, and has a total number of amino acid residues of 100 or less.

    Synthetic peptide for inhibiting expression of type 2 TNF receptor and use thereof
    57.
    发明授权
    Synthetic peptide for inhibiting expression of type 2 TNF receptor and use thereof 有权
    用于抑制2型TNF受体表达的合成肽及其用途

    公开(公告)号：US09480727B2

    公开(公告)日：2016-11-01

    申请号：US14432326

    申请日：2013-10-17

    申请人： TOAGOSEI CO., LTD.

    发明人： Nahoko Kobayashi ,  Tetsuhiko Yoshida

    IPC分类号： A61K38/17

    CPC分类号： A61K38/1716

    摘要： This invention provides an artificially synthesized peptide having an activity to inhibit the expression of type 2 TNF receptor (TNF-R2), and a TNF-R2 expression-inhibiting composition including the peptide as an active ingredient. Also provided is a method for inhibiting the expression of TNF-R2 in cells capable of expressing TNF-R2, by using the peptide. The expression of TNF-R2 in the cells is inhibited, by supplying the cells capable of expressing TNF-R2 with a synthetic peptide essentially including of an amino acid sequence constituting a signal peptide in an amyloid precursor protein (APP) or an amino acid sequence formed by substituting, deleting and/or adding one or several amino acid residues in/from/to the amino acid sequence of the signal peptide.

    摘要翻译： 本发明提供了具有抑制2型TNF受体（TNF-R2）表达的活性的人造合成肽和包含该肽作为活性成分的TNF-R2表达抑制组合物。 还提供了通过使用肽来抑制能够表达TNF-R2的细胞中TNF-R2表达的方法。 通过在基本上包含构成信号肽的氨基酸序列的合成肽供应能够表达TNF-R2的细胞在淀粉状蛋白前体蛋白（APP）或氨基酸序列中抑制TNF-R2的表达 通过在信号肽的氨基酸序列中/从/向其中取代，缺失和/或添加一个或多个氨基酸残基而形成。

    Cell growth-promoting peptide and use thereof
    58.
    发明授权
    Cell growth-promoting peptide and use thereof 有权

    公开(公告)号：US09200257B2

    公开(公告)日：2015-12-01

    申请号：US14341177

    申请日：2014-07-25

    申请人： TOAGOSEI CO., LTD.

    发明人： Tetsuhiko Yoshida ,  Nahoko Kobayashi

    IPC分类号： A61K38/02 ,  A61K38/18 ,  C12N5/02 ,  C12N5/0775 ,  C07K14/47

    摘要： The pharmaceutical composition provided by the present invention comprises at least one pharmaceutically acceptable carrier, and an active ingredient including an artificially synthesized peptide comprises: (A) an amino acid sequence constituting a cell-penetrating peptide and (B) an amino acid sequence constituting the signal peptide in amyloid precursor protein (APP) or an N-terminal partial amino acid sequence or C-terminal partial amino acid sequence from the amino acid sequence constituting that signal peptide.

    ANTIMICROBIAL PEPTIDE AND USE THEREOF
    60.
    发明申请
    ANTIMICROBIAL PEPTIDE AND USE THEREOF 有权
    抗微生物肽及其用途

    公开(公告)号：US20150126434A1

    公开(公告)日：2015-05-07

    申请号：US14397920

    申请日：2013-05-23

    申请人： TOAGOSEI CO., LTD.

    发明人： Nahoko Kobayashi ,  Tetsuhiko Yoshida

    IPC分类号： A01N43/38 ,  C07K7/08 ,  A01N43/36 ,  C07K14/00

    CPC分类号： A01N43/38 ,  A01N37/46 ,  A01N43/36 ,  A61K38/00 ,  C07K7/08 ,  C07K14/00 ,  C07K14/4723 ,  C07K14/705 ,  C07K14/70503 ,  C07K14/70546 ,  C07K14/70596

    摘要： A composition including an artificially synthesized antimicrobial peptide that is not present as a mature peptide in nature. The composition includes an artificially synthesized peptide that has an antimicrobial activity against at least one strain of bacteria or fungi and includes an amino acid sequence represented by any one of SEQ ID NOs: 1 to 6 or an amino acid sequence formed by substituting, deleting and/or adding one, two or three amino acid residues in/from/to the amino acid sequence, and further including at least one species of pharmaceutically acceptable carrier.

    摘要翻译： 包含人造合成的抗微生物肽的组合物，其本质上不作为成熟肽存在。 该组合物包括对至少一种细菌或真菌菌株具有抗微生物活性的人造合成肽，包括由SEQ ID NO：1至6中任一项表示的氨基酸序列或通过取代，缺失和 /或在氨基酸序列中/从氨基酸序列中加入一个，两个或三个氨基酸残基，并且还包括至少一种药学上可接受的载体。