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102 条记录 (耗时 0.029 秒)

    Method for diagnosing inflammatory diseases and infections by the determination of LASP-1 immunoreactivity
    52.
    发明授权
    Method for diagnosing inflammatory diseases and infections by the determination of LASP-1 immunoreactivity 失效
    通过测定LASP-1免疫反应性来诊断炎性疾病和感染的方法

    公开(公告)号：US07405049B2

    公开(公告)日：2008-07-29

    申请号：US10511758

    申请日：2003-04-15

    申请人： Andreas Bergmann ,  Christina Fischer-Schulz ,  Joachim Struck

    发明人： Andreas Bergmann ,  Christina Fischer-Schulz ,  Joachim Struck

    IPC分类号： G01N33/53 ,  G01N33/535 ,  G01N33/68 ,  G01N33/96

    CPC分类号： G01N33/566 ,  G01N33/6893 ,  G01N33/6896 ,  G01N2800/26

    摘要： Method for early diagnosis and diagnosis, for prognosis and assessment of the severity and for therapy-accompanying monitoring of inflammatory diseases and infections, in particular sepsis-like systemic infections and Alzheimer's disease, in which the presence and/or amount of the protein LASP-1 (SEQ ID NO:1) or of the protein LAP-1 (SEQ ID NO:16) or of an immunoreactive fragment of one of these proteins in free and/or protein-bound form is determined, preferably as immunoreactivity, in a biological fluid, or optionally a tissue sample, of a patient, and conclusions are drawn with respect to the presence, the expected course, the severity or the success of a therapy of the inflammatory disease or of the infection from the presence and/or amount of the proteins determined.

    摘要翻译： 用于早期诊断和诊断的方法，用于预测和评估炎性疾病和感染，特别是脓毒症样全身感染和阿尔茨海默氏病的严重性和治疗伴随监测，其中蛋白质LASP- 1（SEQ ID NO：1）或蛋白质LAP-1（SEQ ID NO：16）或这些蛋白质之一的游离和/或蛋白结合形式的免疫反应性片段优选作为免疫反应性 生物流体或任选的组织样品，并且根据存在和/或数量的炎症性疾病或感染的治疗的存在，预期进程，严重程度或成功性得出结论 的蛋白质。

    Receptor binding assay for detecting TSH-receptor auto-antibodies
    55.
    发明授权
    Receptor binding assay for detecting TSH-receptor auto-antibodies 有权
    用于检测TSH-受体自身抗体的受体结合测定

    公开(公告)号：US06537760B1

    公开(公告)日：2003-03-25

    申请号：US09319570

    申请日：1999-08-04

    申请人： Andreas Bergmann ,  Joachim Struck

    发明人： Andreas Bergmann ,  Joachim Struck

    IPC分类号： G01N3353

    CPC分类号： G01N33/76 ,  G01N33/686 ,  Y10S435/963 ,  Y10S435/965 ,  Y10S435/975 ,  Y10S436/811 ,  Y10S530/854

    摘要： In a competitive receptor binding assay for detecting TSH-receptor auto-antibodies in a biological sample, the sample is reacted in a reaction mixture which contains (i) a TSH-receptor or TSH-receptor preparation; (ii) a primary competitor, for example labelled TSH; and (iii) an agent for separating a complex composed of the TSH-receptor and the elements bound thereto of the reaction mixture from the liquid phase. According to the invention, the reaction is carried out in the presence of at least one monoclonal or polyclonal antibody specific against a partial peptide sequence of the TSH eceptor. This specific antibody is used to immobilize a complex of TSH-receptor and primary competitor and/or as secondary competitor for another part of the TSH-receptor auto-antibodies expected in a sample. The primary or secondary competitors are or can be selectively labelled.

    摘要翻译： 在用于检测生物样品中TSH-受体自身抗体的竞争性受体结合测定中，样品在含有（i）TSH受体或TSH-受体制剂的反应混合物中反应; （ii）主要竞争对手，例如标签为TSH; 和（iii）用于从液相中分离由TSH-受体组成的络合物和与反应混合物结合的元素的试剂。 根据本发明，反应在至少一种针对TSH受体的部分肽序列特异性的单克隆或多克隆抗体的存在下进行。 该特异性抗体用于固定TSH受体和主要竞争物的复合物和/或作为样品中预期的另一部分TSH-受体自身抗体的次要竞争物。 主要或次要竞争对手是或可以选择性标签。

    Method for the determination of anti-TSH receptor autoantibodies
    56.
    发明授权
    Method for the determination of anti-TSH receptor autoantibodies 失效
    测定抗TSH受体自身抗体的方法

    公开(公告)号：US5814461A

    公开(公告)日：1998-09-29

    申请号：US596172

    申请日：1996-07-09

    申请人： Andreas Bergmann ,  Joachim Struck ,  Shaul Kornfeld

    发明人： Andreas Bergmann ,  Joachim Struck ,  Shaul Kornfeld

    IPC分类号： G01N33/543 ,  G01N33/564 ,  G01N33/566 ,  G01N33/78 ,  G01N33/53 ,  G01N33/536

    CPC分类号： G01N33/54306 ,  G01N33/564 ,  G01N33/78 ,  G01N2800/24

    摘要： Method for the determination of an analyte in a volume of a fluid sample, in particular of anti-TSH receptor autoantibodies in a patient serum, in which one or more determination reagent(s) which contains or which containa) a predetermined amount of a binder (B) for the analyte (A) and b) a predetermined amount of a competitor (K) which is likewise bound by the binder (B), in such a way that the extent of its binding to the binder (B) is correlated with the simultaneous presence of the analyte (A) and/or its amount in the sample, the competitor (K) being labelled, or being capable of being labelled, with a label which permits its qualitative and/or quantitative determination, is or are added to the sample with the formation of a liquid reaction mixture, wherein c) the liquid reaction mixture is reacted simultaneously or subsequently with a solid phase to which a substance (Imm) for the selective immobilization of the amount of competitor (K) not bound to the binder (B) is bound, and optionally with a labelled reactant (Label) for the immobilized competitor (K), and, after a quantitative separation of the solid phase from the liquid reaction mixture, the amount of the competitor (K) bound to the solid phase is determined in a manner known per se by means of physical and/or chemical detection of the label bound to said competitor, and the determined amount of said competitor (K) is correlated with the presence and/or amount of the analyte (A) in the original sample.

    摘要翻译： PCT No.PCT / EP94 / 02748 Sec。 371日期：1996年7月9日 102（e）日期1996年7月9日PCT 1994年8月18日PCT PCT。 公开号WO95 / 06258 日期1995年3月2日用于测定流体样品体积中特别是患者血清中抗TSH受体自身抗体的分析物的方法，其中一种或多种测定试剂含有或含有a） 预定量的用于分析物（A）的粘合剂（B）和b）同样由粘合剂（B）结合的预定量的竞争剂（K），使得其与 粘合剂（B）与分析物（A）的同时存在和/或其在样品中的量，对象（K）被标记或能够被标记的量相关联，其标签允许其定性和/或 定量测定，或添加到样品中，形成液体反应混合物，其中c）液体反应混合物同时或随后与固相反应，其中用于选择性固定数量的物质（Imm） 竞争对手（K）不绑定到粘合剂（B ），并且任选地具有用于固定化的竞争剂（K）的标记的反应物（Label），并且在从液体反应混合物定量分离固相之后，与固相结合的竞争剂（K）的量 以本身已知的方式通过物理和/或化学检测与所述竞争者结合的标记来确定，并且确定的所述竞争者（K）的量与分析物（A）的存在和/或量相关， 在原始样品中。

    Method for detecting analytes
    58.
    发明授权
    Method for detecting analytes 有权
    检测分析物的方法

    公开(公告)号：US08691595B2

    公开(公告)日：2014-04-08

    申请号：US12666024

    申请日：2008-06-20

    申请人： Andreas Bergmann ,  Joachim Struck

    发明人： Andreas Bergmann ,  Joachim Struck

    IPC分类号： G01N33/549

    CPC分类号： G01N33/54306

    摘要： The subject of this invention is a process for detection of analytes from biological samples comprising the following process steps: a) Preparation of a reversible binding partner 1 that is immobilized on a solid phase, to which an analyte binder is reversibly bonded via a reversible binding partner 2 that is bonded to the analyte binder, whereby the analyte binder is immobilized by binding between the reversible binding partners 1 and 2, b) Addition of the biological sample and binding of the analyte to the reversible immobilized analyte binder in the case that the biological sample contains the analytes, c) Separation of the biological sample, d) Addition of a dissolving buffer, which dissolves the binding between the reversible binding partners 1 and 2, whereby the binding of the analyte to the analyte binder remains optional, and e) Detection of the analyte in the dissolving buffer in the case that the biological sample contains the analytes and determination of the absence of the analyte in the case that the biological sample does not contain the analytes, respectively.

    摘要翻译： 本发明的主题是用于检测来自生物样品的分析物的方法，包括以下过程步骤：a）固定在固相上的可逆结合配偶体1的制备，分析物粘合剂通过可逆结合可逆地结合到其上 配偶体2，其结合到分析物粘合剂上，由此通过可逆结合配偶体1和2之间的结合固定分析物粘合剂，b）添加生物样品并将分析物与可逆固定的分析物结合物结合， 生物样品含有分析物，c）生物样品的分离，d）加入溶解缓冲液，其溶解可逆结合配偶体1和2之间的结合，由此分析物与分析物质粘合剂的结合仍然是可选的，e ）在生物样品含有分析物和确定不存在分析物的情况下，检测溶解缓冲液中的分析物 在生物样品不分别含有分析物的情况下分析物。