公开(公告)号：US20100267141A1

公开(公告)日：2010-10-21

申请号：US12753386

申请日：2010-04-02

申请人： Yan Shi ,  Caroline Desponts ,  Tongxiang Lin ,  Wenlin Li ,  Saiyong Zhu ,  Sheng Ding

发明人： Yan Shi ,  Caroline Desponts ,  Tongxiang Lin ,  Wenlin Li ,  Saiyong Zhu ,  Sheng Ding

IPC分类号： C12N5/0735

CPC分类号： C12N5/0696 ,  C12N15/85 ,  C12N2500/14 ,  C12N2501/01 ,  C12N2501/065 ,  C12N2501/15 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/608 ,  C12N2501/727 ,  C12N2506/08 ,  C12N2506/094 ,  C12N2506/1307 ,  C12N2506/28 ,  C12N2510/00

摘要： The present invention provides for identification and use of small molecules to induce pluripotency in mammalian cells as well as other methods of inducing pluripotency.

摘要翻译： 本发明提供了鉴定和使用小分子以诱导哺乳动物细胞中的多能性以及诱导多能性的其它方法。

公开(公告)号：US20020136709A1

公开(公告)日：2002-09-26

申请号：US09919298

申请日：2001-07-31

申请人： Nucleus Remodeling, Inc.

发明人： Joseph Edward Zahner ,  Asutosh N. Sharda

IPC分类号： A61K048/00 ,  C12N005/08 ,  C12N015/85

CPC分类号： C12N5/0696 ,  A61K48/00 ,  C12N2501/06 ,  C12N2501/065 ,  C12N2501/70 ,  C12N2506/094 ,  C12N2510/00

摘要： Methods for deriving adult pluripotent stem cells from fully differentiated adult somatic cells by in vitro nuclear remodeling are provided. Cells cultured from a variety of tissue sources are treated in vitro to reverse the tissue specific epigenetic chromosomal changes associated with differentiation. Remodeled cells resemble embryonic stem cells by expressing telomerase and demonstrating pluripotency. The cells can be genetically modified to produce heterologous proteins or to correct for genetic defects. Methods for treating a human by implanting in vitro-derived adult pluripotent stem cells (nullNucREMnull cellsnull) and generating engineered tissues for implantation are also disclosed. Advantages to this invention include the non-use of embryos to obtain an unlimited supply of stem cells for therapy and the ability to generate autologous cells and tissues for therapeutic use.

摘要翻译： 提供了通过体外核重塑从完全分化的成体体细胞衍生成体多能干细胞的方法。 从各种组织来源培养的细胞在体外处理以逆转与分化相关的组织特异性表观遗传染色体变化。 改造的细胞通过表达端粒酶和显示多能性来形成胚胎干细胞。 细胞可被遗传修饰以产生异源蛋白质或校正遗传缺陷。 还公开了通过植入体外衍生的成体多能干细胞（“NucREM TM细胞”）和产生用于植入的工程组织来治疗人的方法。 本发明的优点包括不使用胚胎以获得用于治疗的干细胞的无限供应和产生用于治疗用途的自体细胞和组织的能力。

公开(公告)号：US20180327717A1

公开(公告)日：2018-11-15

申请号：US15755812

申请日：2016-08-31

申请人： The Trustees of Columbia University in the City of New York

发明人： Masayuki Yazawa ,  LouJin Song

IPC分类号： C12N5/077 ,  C12N5/074

CPC分类号： C12N5/0657 ,  C12N5/0696 ,  C12N2500/02 ,  C12N2500/90 ,  C12N2500/99 ,  C12N2501/33 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/094 ,  C12N2506/1307 ,  C12N2506/45

摘要： The present invention relates to monolayer cardiac differentiation techniques utilizing defined conditions providing feeder-free monolayer culture systems, serum-based or serum free, and applicable to both healthy control and patient derived stem cells.

公开(公告)号：US10017737B2

公开(公告)日：2018-07-10

申请号：US15297972

申请日：2016-10-19

申请人： GENESIS TECHNOLOGIES LIMITED

发明人： Jan-Eric Ahlfors ,  Rouwayda El-Ayoubi

IPC分类号： C12N5/00 ,  C12N5/0797 ,  C12N5/074 ,  C12N5/0775 ,  C12N5/077 ,  C12N5/0789

CPC分类号： C12N5/0662 ,  A61K35/30 ,  A61K35/545 ,  C12N5/0618 ,  C12N5/0619 ,  C12N5/0623 ,  C12N5/0647 ,  C12N5/0656 ,  C12N5/0657 ,  C12N5/0667 ,  C12N5/0668 ,  C12N5/0676 ,  C12N5/0696 ,  C12N2500/25 ,  C12N2501/06 ,  C12N2501/065 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/395 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/604 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2506/094 ,  C12N2506/11 ,  C12N2506/1307 ,  C12N2506/1346 ,  C12N2506/1384 ,  C12N2510/00

摘要： A method of obtaining a neural multipotent, unipotent or somatic cell, comprising: i) providing a cell of a first type which is not a neural multipotent, unipotent or somatic cell; ii) introducing into the cell of a first type an agent capable of remodeling the chromatin and/or DNA of the cell, wherein the agent capable of remodeling the chromatin and/or DNA is a histone acetylator, an inhibitor of histone deacetylation, a DNA demethylator, and/or a chemical inhibitor of DNA methylation; iii) increasing directly or indirectly the endogenous expression of at least one neural multipotent or unipotent gene regulator in the cell of a first type, to a level at which the gene regulator is capable of driving transformation of the cell of a first type into the neural multipotent, unipotent or somatic cell, wherein the gene regulator is Msi1, Ngn2, Sox2, Ascl1, Zic1 or a combination thereof; and iv) placing or maintaining the cell in a neural cell culture medium and maintaining intracellular levels of the reprogramming agent for a sufficient period of time to allow a neural multipotent, unipotent or somatic cell to be obtained.

公开(公告)号：US20180171303A1

公开(公告)日：2018-06-21

申请号：US15892312

申请日：2018-02-08

申请人： The Scripps Research Institute

发明人： Yan Shi ,  Caroline Desponts ,  Sheng Ding ,  Hongyan Zhou ,  Tongxiang Lin ,  Wenlin Li ,  Saiyong Zhu

IPC分类号： C12N5/074 ,  C12N15/85

CPC分类号： C12N5/0696 ,  C12N15/85 ,  C12N2500/14 ,  C12N2501/01 ,  C12N2501/065 ,  C12N2501/15 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/608 ,  C12N2501/727 ,  C12N2506/08 ,  C12N2506/094 ,  C12N2506/1307 ,  C12N2506/28 ,  C12N2510/00

摘要： The present invention provides for identification and use of small molecules to induce pluripotency in mammalian cells as well as other methods of inducing pluripotency.

公开(公告)号：US09657274B2

公开(公告)日：2017-05-23

申请号：US15069730

申请日：2016-03-14

申请人： The Scripps Research Institute

发明人： Saiyong Zhu ,  Sheng Ding

IPC分类号： C12N5/074

CPC分类号： C12N5/0696 ,  C12N2501/065 ,  C12N2501/15 ,  C12N2501/41 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2506/03 ,  C12N2506/094 ,  C12N2506/28

摘要： The present invention provides for methods, compositions, and kits for producing an induced pluripotent stem cell from a non-pluripotent mammalian cell using a 3′-phosphoinositide-dependent kinase-1 (PDK1) activator or a compound that promotes glycolytic metabolism as well as other small molecules.

公开(公告)号：US20170009210A1

公开(公告)日：2017-01-12

申请号：US15115784

申请日：2015-02-04

申请人： MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH

发明人： Yasuhiro Ikeda ,  Moustafa M.A. El Khatib

IPC分类号： C12N5/071 ,  C12N5/074

CPC分类号： C12N5/0676 ,  C12N5/0678 ,  C12N5/0696 ,  C12N2501/105 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/335 ,  C12N2501/41 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/734 ,  C12N2501/999 ,  C12N2506/094 ,  C12N2506/45 ,  C12N2510/00

摘要： This document provides methods and materials related to making and using differentiated induced pluripotent stem cells. For example, methods and materials for making differentiated induced pluripotent stem cells (e.g., insulin-producing cells) that do not form cancer cells within a mammal (e.g., a human), cells that underwent guided differentiation from induced pluripotent stem cells, compositions containing cells that underwent guided differentiation from induced pluripotent stem cells, and methods for using cells that underwent guided differentiation from induced pluripotent stem cells (e.g., methods for using such cells to treat diabetes or to repair cardiovascular tissue) are provided.

摘要翻译： 本文提供了与使用分化诱导的多能干细胞相关的方法和材料。 例如，用于制造在哺乳动物（例如人）内不形成癌细胞的分化的诱导多能干细胞（例如，产生胰岛素的细胞）的方法和材料，经受诱导的多能干细胞分化的细胞，含有 提供了从诱导多能干细胞引导分化的细胞，以及使用经诱导的多能干细胞引导分化的细胞的方法（例如，使用这种细胞治疗糖尿病或修复心血管组织的方法）。

公开(公告)号：US20160257938A1

公开(公告)日：2016-09-08

申请号：US15069730

申请日：2016-03-14

申请人： The Scripps Research Institute

发明人： Saiyong Zhu ,  Sheng Ding

IPC分类号： C12N5/074

CPC分类号： C12N5/0696 ,  C12N2501/065 ,  C12N2501/15 ,  C12N2501/41 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2506/03 ,  C12N2506/094 ,  C12N2506/28

摘要： The present invention provides for methods, compositions, and kits for producing an induced pluripotent stem cell from a non-pluripotent mammalian cell using a 3′-phosphoinositide-dependent kinase-1 (PDK1) activator or a compound that promotes glycolytic metabolism as well as other small molecules.

摘要翻译： 本发明提供用于使用3'-磷酸肌醇依赖性激酶-1（PDK1）激活剂或促进糖酵解代谢的化合物从非多能哺乳动物细胞产生诱导多能干细胞的方法，组合物和试剂盒，以及 其他小分子。

公开(公告)号：US20150240214A1

公开(公告)日：2015-08-27

申请号：US14637089

申请日：2015-03-03

申请人： The Scripps Research Institute

发明人： Tongxiang Lin ,  Sheng Ding

IPC分类号： C12N5/074

CPC分类号： C12N5/0696 ,  C12N2501/065 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/094 ,  C12N2506/1307 ,  C12N2506/28 ,  C12N2510/00

摘要： The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (>200 fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, non-viral methods for reprogramming human somatic cells.

摘要翻译： 用于产生人诱导多能干细胞（iPSCs）的重编程方法的缓慢动力学和低效率对其在生物医学应用中的实用性施加了主要限制。 在这里，我们描述了一种化学方法，在治疗后7天内显着改善（> 200倍）来自人成纤维细胞的iPSC生成的效率。 这将为重新编程人体细胞提供更安全，更有效，非病毒的方法的基础。

公开(公告)号：US20150157662A1

公开(公告)日：2015-06-11

申请号：US14394882

申请日：2013-04-18

申请人： Board of Regents, The University of Texas System

发明人： Elsa R. Flores ,  Deepavali Chakravarti

IPC分类号： A61K35/12 ,  C12N15/113 ,  C12N5/074

CPC分类号： A61K35/12 ,  C12N5/0696 ,  C12N15/113 ,  C12N2310/14 ,  C12N2320/30 ,  C12N2501/40 ,  C12N2501/998 ,  C12N2506/094 ,  C12N2510/00

摘要： The present invention generally concerns particular methods and compositions for generation of induced pluripotent stem cells. In particular aspects, induced pluripotent stem cells are generated from adult somatic cells following downregulation of a particular gene of interest. In some embodiments, induced pluripotent stem cells are generated from keratinocytes upon downregulation of ΔNp63 or DGCR8.

摘要翻译： 本发明一般涉及用于产生诱导多能干细胞的具体方法和组合物。 在特定方面，诱导的多能干细胞是在特定的感兴趣基因下调之后从成体体细胞产生的。 在一些实施方案中，诱导的多能干细胞在下调“Dgr; Np63”或“DGCR8”时由角质细胞产生。