公开(公告)号：US07990532B2

公开(公告)日：2011-08-02

申请号：US12718362

申请日：2010-03-05

Applicant: Jason Neiss ,  Patrick Treado ,  Robert C. Schweitzer

Inventor： Jason Neiss ,  Patrick Treado ,  Robert C. Schweitzer

IPC: G01J3/44

CPC classification number: G01J3/28

Abstract: System and method for assessing the occurrence of an unknown substance in a sample that comprises multiple entities. A reference library is provided comprising a plurality of reference data sets representative of at least one known substance. A first feature of the entities is assessed wherein the first feature is characteristic of the unknown substance. A region of interest is selected wherein the region of interest comprises at least one entity exhibiting the first feature. A spatially accurate wavelength resolved Raman image is obtained wherein each pixel in the image is the Raman spectrum of the sample at the corresponding location. The spatially accurate wavelength resolved image is assessed to thereby identify the unknown substance.

Abstract translation: 用于评估包含多个实体的样品中未知物质的发生的系统和方法。 提供了包括代表至少一种已知物质的多个参考数据集的参考文库。 评估实体的第一特征，其中第一特征是未知物质的特征。 选择感兴趣区域，其中感兴趣区域包括展示第一特征的至少一个实体。 获得空间精确的波长分辨拉曼图像，其中图像中的每个像素是相应位置处样品的拉曼光谱。 评估空间上准确的波长分辨图像，从而识别未知物质。

公开(公告)号：US20110007309A1

公开(公告)日：2011-01-13

申请号：US12834462

申请日：2010-07-12

Applicant: Shona Stewart ,  John Maier ,  Patrick Treado

Inventor： Shona Stewart ,  John Maier ,  Patrick Treado

IPC: G01J3/44

CPC classification number: G01N21/65 ,  G01J3/2823 ,  G01J3/44 ,  G01N21/658 ,  G01N33/1826

Abstract: A method for assessing the presence of a pathogenic microorganism in a sample. A sample is illuminated to thereby produce a first plurality of interacted photons which may be scattered, emitted, reflected and/or absorbed by the sample. The first plurality of interacted photons are assessed to thereby generate a Raman data set representative of the sample. This Raman data set is analyzed to thereby determine at least one of: the presence of a pathogenic microorganism in said sample and the absence of a pathogenic microorganism in said sample. The Raman data set may comprise at least one of a Raman spectrum and/or a Raman chemical image representative of the sample. The analysis may comprise comparing said Raman data set to at least one reference Raman data set representative of a known sample. This may be achieved using a chemometric technique.

Abstract translation: 用于评估样品中病原微生物的存在的方法。 照射样品从而产生可被样品散射，发射，反射和/或吸收的第一多个相互作用的光子。 评估第一组多个相互作用的光子，从而产生代表样品的拉曼数据集。 分析该拉曼数据集，从而确定所述样品中病原微生物的存在和所述样品中不存在致病微生物中的至少一种。 拉曼数据集可以包括代表样品的拉曼光谱和/或拉曼化学图像中的至少一个。 分析可以包括将所述拉曼数据集与代表已知样本的至少一个参考拉曼数据集进行比较。 这可以使用化学计量技术来实现。

公开(公告)号：US20080151225A1

公开(公告)日：2008-06-26

申请号：US11351333

申请日：2006-02-09

Applicant: Patrick Treado ,  Charles Gardner ,  John Maier

Inventor： Patrick Treado ,  Charles Gardner ,  John Maier

IPC: G01N21/00 ,  G01J3/44 ,  G01N21/65

CPC classification number: G01J3/44 ,  G01N21/65

Abstract: A system method for depositing a sample of a threat agent is deposited onto a substrate. The threat agent is illuminated via an illumination source along an optical path with a plurality of photons to thereby produce photons transmitted, reflected, emitted or Raman scattered by the threat agent. An optical system collects elastic scatter photons produced by the threat agent and at least one of photons transmitted, reflected, emitted or Raman scattered by the threat agent, wherein said illumination source is located along an optical path, and said substrate is located along a plane wherein the optical path is at an angle other than 90° with respect to the substrate plane. The depth of field of the optical system is extended by passing at least one of the following through a phase mask: elastic scattered photons, and photons transmitted, reflected, emitted or Raman scattered by the threat agent.

Abstract translation: 将用于沉积威胁剂的样品的系统方法沉积在基底上。 通过沿着具有多个光子的光路的照明源照射威胁剂，从而产生被威胁剂散射的透射，反射，发射或拉曼的光子。 光学系统收集由威胁剂产生的弹性散射光子和由威胁剂散射的发射，反射，发射或拉曼的光子中的至少一种，其中所述照明源沿着光路定位，并且所述衬底沿着平面 其中所述光路相对于所述基板平面成90°以外的角度。 通过使以下各项中的至少一个通过相位掩模：弹性散射光子和由威胁剂散发的透射，反射，发射或拉曼的光子来延长光学系统的景深。

公开(公告)号：US20080088844A1

公开(公告)日：2008-04-17

申请号：US11902874

申请日：2007-09-26

Applicant: Xinghua Wang ,  Thomas Voigt ,  David Tuschel ,  Chenhui Wang ,  Patrick Treado

Inventor： Xinghua Wang ,  Thomas Voigt ,  David Tuschel ,  Chenhui Wang ,  Patrick Treado

IPC: G01N21/25

CPC classification number: G01N21/65 ,  G01J3/02 ,  G01J3/0291 ,  G01J3/36 ,  G01J3/44 ,  G01N21/359 ,  G01N21/59 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/656

Abstract: The disclosure generally relates to a multimode imaging apparatus for simultaneously obtaining multiple wavelength-discriminative spectral images of a sample. In one embodiment, the apparatus includes an image selector having a rotator assembly, the rotator assembly housing a first plurality of optical components, the image selector adapted to receive a illuminating photons having a first wavelength and direct the illuminating photons to the sample, the image selector adapted to receive illuminating photons interacted with the sample and selectively direct said interacted photons to one of a plurality of detection sources; a microscope turret housing a second plurality of components, the microscope turret adapted to receive illuminating photons having a second wavelength and direct the photons to the sample; the microscopic turret adapted to receive illuminating photons interacted with the sample and selectively direct said interacted photons to one of a plurality of detection sources; wherein substantially all of the interacted photons are selectively directed one of a plurality of detection sources to form multiple wavelength discriminative spectral images of the sample simultaneously.

公开(公告)号：US20070263290A1

公开(公告)日：2007-11-15

申请号：US11744797

申请日：2007-05-04

Applicant: Patrick Treado ,  Matthew Nelson ,  Scott Keitzer ,  Ryan Smith

Inventor： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer ,  Ryan Smith

IPC: G02B5/04 ,  G02B3/00 ,  G02B5/22

CPC classification number: G02B6/06 ,  A61B1/00165 ,  A61B1/043 ,  A61B1/063 ,  A61B1/0638 ,  A61B1/0646 ,  A61B1/07 ,  A61B5/0075 ,  A61B5/0084 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/0218 ,  G01J3/0227 ,  G01J3/024 ,  G01J3/0256 ,  G01J3/0262 ,  G01J3/0286 ,  G01J3/44 ,  G01J2003/1213

Abstract: A fiberscope device is disclosed which is suitable for video imaging, laser Raman spectroscopy and laser Raman spectroscopic (i.e. chemical) imaging. The fiberscope design minimizes fiber background interference arising from the laser delivery fiber optic and the coherent fiber optic light gathering bundle while maintaining high light throughput efficiency through the use of integrated spectral filters. In the fiberscope design, the laser delivery fiber optic is offset from the coherent fiber optic light gathering bundle. The laser delivery field is captured entirely by the light gathering field of view of the coherent fiber bundle. The fiberscope incorporates spectral filter optical elements that provide environmental insensitivity, particularly to temperature and moisture. The fiberscope is suited to the analysis of a wide range of condensed phase materials (solids and liquids), including the analysis of biological materials such as breast tissue lesions and arterial plaques, in such a manner to delineate abnormal from normal tissues.

Abstract translation: 公开了适用于视频成像，激光拉曼光谱和激光拉曼光谱（即化学）成像的纤维内窥镜装置。 纤维镜设计可以最大限度地减少激光传输光纤和相干光纤采集光束产生的光纤背景干扰，同时通过使用集成光谱滤波器保持高光通量效率。 在纤维镜设计中，激光传输光纤与相干光纤聚光束偏移。 激光输送场完全由相干光纤束的聚光场捕获。 纤维镜包含光谱滤光器光学元件，其提供环境不敏感性，特别是对温度和湿度。 纤维内窥镜适用于分析各种凝聚相材料（固体和液体），包括分析生物材料如乳房组织损伤和动脉斑块，以便从正常组织中描绘异常。

公开(公告)号：US20070182959A1

公开(公告)日：2007-08-09

申请号：US10556038

申请日：2005-06-30

Applicant: John Maier ,  Patrick Treado ,  David Tuschel ,  Thomas Voigt

Inventor： John Maier ,  Patrick Treado ,  David Tuschel ,  Thomas Voigt

IPC: G01J3/44 ,  G01N21/65

CPC classification number: G01N21/6456 ,  G01J3/2803 ,  G01J3/32 ,  G01J3/36 ,  G01J3/44 ,  G01N21/359 ,  G01N21/6486 ,  G01N21/65 ,  G01N21/658 ,  G01N2021/656

Abstract: The invention relates to methods of dynamic chemical imaging, including methods of cellular imaging. The method comprises illuminating at least a portion of a cell with substantially monochromatic light and assessing Raman-shifted light scattered from the illuminated portion at a plurality of discrete times. The Raman-shifted light can be assessed at a plurality of Raman shift (RS) values at each of the discrete times, and the RS values can be selected to be characteristic of a pre-selected component at each of the discrete times. Multivariate analysis of Raman spectral features of the images thus obtained can yield the location and chemical identity of components in the field of view. This information can be combined or overlaid with other spectral data (e.g., a visible microscopic image) obtained from the field of view.

Abstract translation: 本发明涉及动态化学成像方法，包括细胞成像方法。 该方法包括以基本上单色的光照亮电池的至少一部分，并且在多个离散时间评估从照射部分散射的拉曼偏移光。 可以在每个离散时间处以多个拉曼位移（RS）值评估拉曼移位的光，并且可以将每个离散时间的RS值选择为预选择的分量的特征。 如此获得的图像的拉曼光谱特征的多变量分析可以产生视场中部件的位置和化学特性。 该信息可以与从视野获得的其他光谱数据（例如，可见显微镜图像）组合或覆盖。

公开(公告)号：US20070145258A1

公开(公告)日：2007-06-28

申请号：US11640197

申请日：2006-12-18

Applicant: Matthew Nelson ,  Patrick Treado

Inventor： Matthew Nelson ,  Patrick Treado

IPC: G01D18/00

CPC classification number: G01J3/28 ,  A61B5/0059 ,  A61B2560/0223 ,  G01J2003/2866

Abstract: The disclosure generally relates to a method and apparatus for automated spectral calibration of a spectroscopy device. In one embodiment, the disclosure relates to a method for simultaneous calibration and spectral imaging of a sample by: simultaneously illuminating the sample and a calibrant with a plurality of illuminating photons; receiving, at the spectrometer, a first plurality of photons collected from the sample and a second plurality of photons collected from the calibrant; forming a calibrant spectrum from the first plurality of collected photons and a sample spectrum from the second plurality of collected photons; comparing the calibrant spectrum with a reference spectrum of the calibrant to determine a wavelength-shift in the calibrant spectrum; applying the wavelength-shift to the sample spectrum to obtain a calibrated sample spectrum.

Abstract translation: 本发明一般涉及一种用于光谱设备的自动光谱校准的方法和装置。 在一个实施方案中，本公开涉及通过以下步骤同时校准和光谱成像的方法：同时用多个照明光子照射样品和校准物; 在光谱仪处接收从样品收集的第一组多个光子和从校准物收集的第二组多个光子; 从所述第一多个收集的光子形成校准谱，以及来自所述第二多个收集的光子的样品光谱; 将校准谱与校准物的参考光谱进行比较，以确定校准谱中的波长漂移; 将波长偏移应用于样本光谱以获得校准的样本光谱。

公开(公告)号：US20060281068A1

公开(公告)日：2006-12-14

申请号：US11269596

申请日：2005-11-09

Applicant: John Maier ,  Shona Stewart ,  Patrick Treado

Inventor： John Maier ,  Shona Stewart ,  Patrick Treado

IPC: C12Q1/00 ,  C12Q1/70 ,  C12Q1/68 ,  G06F19/00 ,  G01J3/44

CPC classification number: G01N21/65 ,  G01N2021/656

Abstract: Raman molecular imaging (RMI) is used to detect mammalian cells of a particular phenotype. For example the disclosure includes the use of RMI to differentiate between normal and diseased cells or tissues, e.g., cancer cells as well as in determining the grade of said cancer cells. In a preferred embodiment benign and malignant lesions of bladder and other tissues can be distinguished, including epithelial tissues such as lung, prostate, kidney, breast, and colon, and non-epithelial tissues, such as bone marrow and brain. Raman scattering data relevant to the disease state of cells or tissue can be combined with visual image data to produce hybrid images which depict both a magnified view of the cellular structures and information relating to the disease state of the individual cells in the field of view. Also, RMI techniques may be combined with visual image data and validated with other detection methods to produce confirm the matter obtained by RMI.

Abstract translation: 拉曼分子成像（RMI）用于检测特定表型的哺乳动物细胞。 例如，本公开包括使用RMI来区分正常和患病细胞或组织，例如癌细胞，以及确定所述癌细胞的等级。 在一个优选实施方案中，可以区分膀胱和其他组织的良性和恶性病变，包括上皮组织如肺，前列腺，肾，乳腺和结肠以及非上皮组织，例如骨髓和脑。 可以将与细胞或组织的疾病状态相关的拉曼散射数据与视觉图像数据组合，以产生描绘细胞结构的放大视图和与视野中的各个细胞的疾病状态有关的信息的混合图像。 此外，RMI技术可以与可视图像数据组合，并用其他检测方法验证，以确定RMI获得的事项。

公开(公告)号：US20060192956A1

公开(公告)日：2006-08-31

申请号：US11257219

申请日：2005-10-24

Applicant: Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

Inventor： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

IPC: G01J3/28

CPC classification number: G01N21/359 ,  G01J3/2823 ,  G02B21/0016 ,  G02B21/06

Abstract: A chemical imaging system is provided which uses a near infrared radiation microscope. The system includes an illumination source which illuminates an area of a sample using light in the near infrared radiation wavelength and light in the visible wavelength. A multitude of spatially resolved spectra of transmitted, reflected, emitted or scattered near infrared wavelength radiation light from the illuminated area of the sample is collected and a collimated beam is produced therefrom. A near infrared imaging spectrometer is provided for selecting a near infrared radiation image of the collimated beam. The filtered images are collected by a detector for further processing. The visible wavelength light from the illuminated area of the sample is simultaneously detected providing for the simultaneous visible and near infrared chemical imaging analysis of the sample. Two efficient means for performing three dimensional near infrared chemical imaging microscopy are provided.

Abstract translation: 提供使用近红外辐射显微镜的化学成像系统。 该系统包括使用近红外辐射波长的光和可见波长的光来照射样品的区域的照明源。 收集来自样品的照射区域的透射，反射，发射或散射的近红外波长辐射光的多个空间解析光谱，并从其中产生准直光束。 提供近红外成像光谱仪用于选择准直光束的近红外辐射图像。 滤波后的图像由检测器收集以进一步处理。 同时检测来自样品的照射区域的可见波长的光，提供样品的同时可见和近红外化学成像分析。 提供了三维近红外化学成像显微镜的两种有效手段。

公开(公告)号：US20060151702A1

公开(公告)日：2006-07-13

申请号：US11366129

申请日：2006-03-02

Applicant: Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

Inventor： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

IPC: G01N21/35

CPC classification number: G01N21/359 ,  G01J3/2823 ,  G02B21/0016 ,  G02B21/06

Abstract: A chemical imaging system is provided which uses a near infrared radiation microscope. The system includes an illumination source which illuminates an area of a sample using light in the near infrared radiation wavelength and light in the visible wavelength. A multitude of spatially resolved spectra of transmitted, reflected, emitted or scattered near infrared wavelength radiation light from the illuminated area of the sample is collected and a collimated beam is produced therefrom. A near infrared imaging spectrometer is provided for selecting a near infrared radiation image of the collimated beam. The filtered images are collected by a detector for further processing. The visible wavelength light from the illuminated area of the sample is simultaneously detected providing for the simultaneous visible and near infrared chemical imaging analysis of the sample. Two efficient means for performing three dimensional near infrared chemical imaging microscopy are provided.