公开(公告)号：US09158118B2

公开(公告)日：2015-10-13

申请号：US13656457

申请日：2012-10-19

Applicant: ACEA BIOSCIENCES, INC

Inventor： Nan Li ,  Ye Chen ,  Xiaobo Wang

IPC: G02B27/10 ,  G01J3/443 ,  G02B5/04 ,  G02B27/14 ,  G01J3/14 ,  G01J3/36 ,  G02B5/28

CPC classification number: G02B27/1006 ,  G01J3/14 ,  G01J3/36 ,  G01J3/443 ,  G02B5/04 ,  G02B5/285 ,  G02B27/141 ,  G02B27/142

Abstract: A prism including a substrate faceted to provide a plurality of flat surfaces, wherein at least two of the plurality of surfaces, each including a filter coating, form at least two filter surfaces, wherein each filter surface selectively permits passage of a predetermined wavelength and reflects remaining wavelengths along an optical path towards another of the plurality of surfaces, optionally another filter surface, wherein an angle of incidence of each of the plurality of surfaces along the optical path is equal or nearly equal. An apparatus incorporating the prism and its use for splitting a light spectrum into a plurality of wavelengths or wavelength ranges.

Abstract translation: 一种棱镜，包括面以提供多个平坦表面的基板，其中，所述多个表面中的至少两个表面，每个包括过滤器涂层，形成至少两个过滤器表面，其中每个过滤器表面选择性地允许预定波长的通过并反射 沿着光路的剩余波长朝向多个表面中的另一个，可选地另外的滤光器表面，其中沿着光路的多个表面中的每一个的入射角相等或接近相等。 一种结合棱镜的装置及其用于将光谱分解成多个波长或波长范围的用途。

公开(公告)号：US09034885B2

公开(公告)日：2015-05-19

申请号：US13843554

申请日：2013-03-15

Applicant: ACEA BIOSCIENCES INC.

Inventor： Xiao Xu ,  Xiaobo Wang ,  Long Mao ,  Li Zhao ,  Biao Xi

IPC: A01N43/90 ,  A61K31/519 ,  C07D487/04 ,  A61K45/06

CPC classification number: A61K31/519 ,  A61K45/06 ,  C07D487/04 ,  A61K2300/00

Abstract: The present invention relates to certain pyrrolopyrimidine derivatives, pharmaceutical compositions containing them, and methods of using them, including methods for the treatment of tumors and related diseases related to the dysregulation of kinase (such as EGFR (including HER), Alk, PDGFR, but not limited to) pathways.

Abstract translation: 本发明涉及某些吡咯并嘧啶衍生物，含有它们的药物组合物及其使用方法，包括治疗肿瘤的方法和与激酶失调相关的相关疾病（如EGFR（包括HER），Alk，PDGFR），但是 途径。

公开(公告)号：US20140203818A1

公开(公告)日：2014-07-24

申请号：US14092927

申请日：2013-11-28

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Wei Ouyang ,  Nan Li ,  Tianxing Wang ,  Xiaoyu Zhang ,  Xiao Xu ,  Yama A. Abassi

IPC: G01N27/02

CPC classification number: G01N27/02 ,  G01N33/4836

Abstract: Devices, systems and methods for monitoring excitable cells, such as cardiomyocytes, on microelectrode arrays that couple the electro-stimulation of excitable cells to induce or regulate cardiomyocyte beating and the simultaneous measurement of impedance and extracellular recording to assess changes in cardiomyocyte beating, viability, morphology or electrophysical properties in response to a plurality of treatments.

Abstract translation: 用于监测微电极阵列上的可兴奋细胞（例如心肌细胞）的装置，系统和方法，其耦合可刺激细胞的电刺激以诱导或调节心肌细胞搏动，以及同时测量阻抗和细胞外记录以评估心肌细胞跳动，活力， 响应于多种处理的形态或电物理性质。

公开(公告)号：US20110300569A1

公开(公告)日：2011-12-08

申请号：US13109809

申请日：2011-05-17

Applicant: Nan Li ,  Xiaobo Wang ,  Yama A. Abassi ,  Biao Xi ,  Wen Fu Zhang ,  Xiao Xu

Inventor： Nan Li ,  Xiaobo Wang ,  Yama A. Abassi ,  Biao Xi ,  Wen Fu Zhang ,  Xiao Xu

IPC: C12Q1/02

CPC classification number: G01N33/48728 ,  G01N33/4836 ,  G01N33/5008 ,  G01N33/5014 ,  G01N33/5061 ,  G01N33/5073 ,  G01N2800/326 ,  G01N2800/52

Abstract: A method of determining one or more beating parameters for use in cardiomyocyte beating analysis including: providing a cell analysis device including wells, each well including a sensor capable of monitoring beating of cardiomyoctes in millisecond time resolution; adding cardiomyocytes to the wells; monitoring the beating of the cardiomyocytes in millisecond time resolution to obtain a plurality of beating measurements; and calculating one or more beating parameters from the plurality of beating measurements.

Abstract translation: 一种确定用于心肌细胞搏动分析的一个或多个跳动参数的方法，包括：提供包括孔的细胞分析装置，每个孔包括能以毫秒时间分辨率监测心脏跳动的传感器; 向心脏中加入心肌细胞; 以毫秒时间分辨率监测心肌细胞的搏动以获得多次打浆测量; 以及从所述多次跳动测量中计算一个或多个跳动参数。

公开(公告)号：US20110117542A1

公开(公告)日：2011-05-19

申请号：US13011725

申请日：2011-01-21

Applicant: Yama Abassi ,  Xiaobo Wang ,  Xiao Xu

Inventor： Yama Abassi ,  Xiaobo Wang ,  Xiao Xu

IPC: C12Q1/02 ,  C12Q1/70 ,  C12Q1/68

CPC classification number: C12Q1/025 ,  C12Q1/002 ,  G01N27/02 ,  G01N33/4836 ,  G01N33/5014 ,  G01N33/5438

Abstract: The invention provides methods of investigating a mechanism of action of a compound, which includes providing a device for monitoring cell-substrate impedance; attaching the device to an impedance analyzer; adding cells to two or more wells; adding a test compound to at least one of the wells and providing at least one control well; monitoring impedance of the wells to obtain a series of impedance measurements; plotting impedance measurements to obtain impedance curves and comparing the impedance curves to determine a time frame at which the test compound has a significant effect on cell growth or behavior. Determining the time frame provides information on changes in cell status in response to the test compound, including cell attachment or adhesion status, cell growth or proliferation status, the number of viable or dead cells, cytoskeletal organization or structure, and the number of cells going through apoptosis or necrosis.

Abstract translation: 本发明提供了研究化合物作用机理的方法，其包括提供用于监测细胞 - 基底阻抗的装置; 将设备连接到阻抗分析仪; 将细胞添加到两个或更多个孔中; 向至少一个孔中加入测试化合物并提供至少一个对照孔; 监测井的阻抗以获得一系列阻抗测量; 绘制阻抗测量以获得阻抗曲线并比较阻抗曲线以确定测试化合物对细胞生长或行为具有显着影响的时间框架。 确定时间框架提供了响应于测试化合物的细胞状态变化的信息，包括细胞附着或粘附状态，细胞生长或增殖状态，活细胞或死细胞的数量，细胞骨架组织或结构以及细胞数目 通过凋亡或坏死。

公开(公告)号：US20100191016A1

公开(公告)日：2010-07-29

申请号：US12413385

申请日：2009-03-27

Applicant: Haoyun An ,  Xiaopeng Mo

Inventor： Haoyun An ,  Xiaopeng Mo

IPC: C07C321/20

CPC classification number: C07C319/24 ,  C07C319/28 ,  C07C323/07

Abstract: The invention provides a synthetic and manufacturing process for the preparation of the anticancer drug, fluorapacin, bis(4-fluorobenzyl)trisulfide, and related trisulfide derivatives on large scale. Also provided are processes for the purification and isolation of fluorapacin having high purity and improved stability.

Abstract translation: 本发明提供了大规模制备抗癌药物，氟卡宾，双（4-氟苄基）三硫化物和相关三硫​​化物衍生物的合成和制备方法。 还提供了用于纯化和分离具有高纯度和改善的稳定性的氟沙平的方法。

公开(公告)号：US07622507B2

公开(公告)日：2009-11-24

申请号：US11110203

申请日：2005-04-20

Applicant: Xiao Xu ,  Haoyun An ,  Xiaobo Wang

Inventor： Xiao Xu ,  Haoyun An ,  Xiaobo Wang

IPC: A61K31/105 ,  C07C321/16

CPC classification number: C07C323/07 ,  C07C321/20 ,  C07C323/16 ,  C07C323/20

Abstract: The present invention provides substituted di-, tri-, tetra- and penta-sulfide compounds and compositions, and methods of using the same for the treatment and/or prevention of a cell proliferative disorder. The present invention also provides methods for preparing trisulfide compounds and compositions.

Abstract translation: 本发明提供取代的二，三，四和五硫化物化合物和组合物，及其用于治疗和/或预防细胞增殖性疾病的方法。 本发明还提供了制备三硫化物和组合物的方法。

公开(公告)号：US20090205201A1

公开(公告)日：2009-08-20

申请号：US12325530

申请日：2008-12-01

Applicant: Xiao Xu ,  Xiaobo Wang

Inventor： Xiao Xu ,  Xiaobo Wang

IPC: H01R43/00 ,  B23K26/00

CPC classification number: G01N27/02 ,  C12M23/12 ,  C12M41/36 ,  G01N27/3276 ,  Y10T29/49117

Abstract: A device for detecting cells and/or molecules on an electrode surface is disclosed. The device detects cells and/or molecules through measurement of impedance changes resulting from the cells and/or molecules. A disclosed embodiment of the device includes a substrate having two opposing ends along a longitudinal axis. A plurality of electrode arrays are positioned on the substrate. Each electrode array includes at least two electrodes, and each electrode is separated from at least one adjacent electrode in the electrode array by an expanse of non-conductive material. The electrode has a width at its widest point of more than about 1.5 and less than about 10 times the width of the expanse of non-conductive material. The device also includes electrically conductive traces extending substantially longitudinally to one of the two opposing ends of the substrate without intersecting another trace. Each trace is in electrical communication with at least one of the electrode arrays.

Abstract translation: 公开了一种用于检测电极表面上的电池和/或分子的装置。 该装置通过测量由细胞和/或分子产生的阻抗变化来检测细胞和/或分子。 所述装置的公开实施例包括具有沿着纵向轴线的两个相对端部的基板。 多个电极阵列位于基板上。 每个电极阵列包括至少两个电极，并且每个电极由电极阵列中的至少一个相邻的电极通过一片非导电材料分开。 电极的最宽点处的宽度大于非导电材料的宽度的大约1.5倍且小于约10倍。 该器件还包括基本上纵向延伸到衬底的两个相对端之一的导电迹线，而不与另一迹线相交。 每个迹线与至少一个电极阵列电连通。

公开(公告)号：US10725023B2

公开(公告)日：2020-07-28

申请号：US16237382

申请日：2018-12-31

Applicant: ACEA Biosciences, Inc.

Inventor： Yama A. Abassi ,  Naichen Yu ,  Josephine Atienza ,  Xiao Xu ,  Xiaobo Wang

IPC: G01N33/50 ,  G01N33/543 ,  G01N27/02 ,  C12Q1/00

Abstract: A method of identifying a therapeutic compound for treating cancer in a human subject, the method including: providing a device that measures cell-substrate impedance; culturing cancer cells in the at least two wells, wherein the cancer cells are obtained from a human subject and have a receptor tyrosine kinase (RTK) pathway; adding to a first well a proposed therapeutic compound that affects an RTK pathway and an RTK stimulating factor for the RTK pathway to form a test well, and adding to another well the RTK stimulating factor to form a control well; continuously monitoring cell-substrate impedance of the at least two wells; and determining a difference in impedance or optionally in cell index between the test well and control well; and if significantly different, concluding the proposed therapeutic compound is therapeutically active in the RTK pathway within the cancer cells of the human subject.

公开(公告)号：US10261080B2

公开(公告)日：2019-04-16

申请号：US15650896

申请日：2017-07-15

Applicant: ACEA Biosciences, Inc.

Inventor： Nan Li ,  Lingbo Kong ,  Jian Wu ,  Ting Cheng ,  Yangde Qin ,  Xiaobo Wang

IPC: G01N33/569 ,  G01N15/14 ,  G01N15/10

Abstract: An optical engine its use in a bench top flow cytometer, the optical engine having a set of lasers, each focused horizontally along an x-axis to a same horizontal position and vertically along a y-axis to a different vertical position along a same excitation plane of a flow cell, a set of optics that separate fluorescence of a same wavelength range into different locations in a focal plane of collection optics according to the different lasers by which the fluorescent light is excited; and a detector that selectively detects light from the different locations thereby distinguishing between fluorescence emitted within the same wavelength range as excited by the different lasers.