公开(公告)号：US20150167065A1

公开(公告)日：2015-06-18

申请号：US14106264

申请日：2013-12-13

Applicant: General Electric Company

Inventor： John Richard Nelson ,  David Roger Moore ,  Bing Li ,  Robert Scott Duthie ,  Patrick McCoy Spooner

IPC: C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q1/6853 ,  C12Q2521/307 ,  C12Q2531/119

Abstract: Provided herein are methods for amplification a target dsDNA that is impregnated within a porous matrix using endonuclease-assisted DNA amplification. The amplicons may be subsequent detected within the porous matrix or may be eluted out of the porous matrix. Methods for extracting a genetic material from a biological sample using endonuclease-assisted DNA amplification within a porous matrix are also provided.

Abstract translation: 本文提供了使用内切核酸酶辅助的DNA扩增来扩增浸渍在多孔基质内的靶dsDNA的方法。 可以在多孔基质内随后检测扩增子，或者可以从多孔基质中洗脱。 还提供了在多孔基质内使用内切核酸酶辅助的DNA扩增从生物样品中提取遗传物质的方法。

公开(公告)号：US20150118683A1

公开(公告)日：2015-04-30

申请号：US14068532

申请日：2013-10-31

Applicant: General Electric Company

Inventor： Bing Li ,  Gregory Andrew Grossmann ,  David Roger Moore

IPC: C12Q1/68

CPC classification number: C12N15/1006

Abstract: A solid substrate for biological sample storage under dry-state and elution of biomolecules is provided. The dry, solid substrate comprises a surface modified with a plurality of hydrophilic groups; and the substrate is comprised of one or more protein denaturing agents impregnated therein under a substantially dry state. A method for elution of biomolecules from biological samples is also provided. The compositions disclosed herein provide for enhanced elution and recovery of biomolecules, such as nucleic acids, from the sample. The sample is disposed on a substrate, dried to a substantially dry state; eluted from the biological sample dried on the substrate by rehydrating the substrate in an elution buffer.

Abstract translation: 提供了一种在干态下生物样品储存和生物分子洗脱的固体底物。 干燥的固体基质包括用多个亲水基团改性的表面; 并且底物由在基本干燥状态下浸渍的一种或多种蛋白质变性剂组成。 还提供了用于从生物样品中洗脱生物分子的方法。 本文公开的组合物提供来自样品的生物分子（例如核酸）的增强的洗脱和回收。 将样品置于基材上，干燥至基本干燥状态; 通过在洗脱缓冲液中再水化底物，从在底物上干燥的生物样品中洗脱。