公开(公告)号：US10539523B2

公开(公告)日：2020-01-21

申请号：US14092927

申请日：2013-11-28

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Wei Ouyang ,  Nan Li ,  Tianxing Wang ,  Xiaoyu Zhang ,  Xiao Xu ,  Yama A. Abassi

IPC: G01N27/02

Abstract: Devices, systems and methods for monitoring excitable cells, such as cardiomyocytes, on microelectrode arrays that couple the electro-stimulation of excitable cells to induce or regulate cardiomyocyte beating and the simultaneous measurement of impedance and extracellular recording to assess changes in cardiomyocyte beating, viability, morphology or electrophysical properties in response to a plurality of treatments.

公开(公告)号：US10533985B2

公开(公告)日：2020-01-14

申请号：US16020679

申请日：2018-06-27

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Yama A. Abassi ,  Biao Xi ,  Wen Fu Zhang ,  Xiao Xu

IPC: G01N33/483 ,  G01N33/487

Abstract: A system for monitoring cell-substrate impedance of excitable cells and monitoring the beating cycle of cardiomyocytes, the system including: a device for monitoring cell-substrate impedance at 20 millisecond time resolution, the device having one or more wells on a nonconductive substrate, wherein at least one well of the one or more wells comprises an electrode array fabricated on the substrate for measurement of cell-substrate impedance at 20 millisecond time resolution; an impedance analyzer that measures cell-substrate impedance from the at least one well at 20 millisecond time resolution; electronic circuitry that electrically connects the electrode array from the at least one well to the impedance analyzer; and a software program that analyzes the measured cell-substrate impedance.

公开(公告)号：US20190195861A1

公开(公告)日：2019-06-27

申请号：US16237382

申请日：2018-12-31

Applicant: ACEA Biosciences, Inc.

Inventor： Yama A. Abassi ,  Naichen Yu ,  Josephine Atienza ,  Xiao Xu ,  Xiaobo Wang

IPC: G01N33/50 ,  G01N27/02 ,  G01N33/543 ,  C12Q1/00

CPC classification number: G01N33/5041 ,  C12Q1/002 ,  G01N27/021 ,  G01N33/5014 ,  G01N33/5438 ,  G01N2333/4716 ,  G01N2333/705 ,  G01N2333/91205 ,  G01N2500/04 ,  G01N2500/10

Abstract: A method of identifying a therapeutic compound for treating cancer in a human subject, the method including: providing a device that measures cell-substrate impedance; culturing cancer cells in the at least two wells, wherein the cancer cells are obtained from a human subject and have a receptor tyrosine kinase (RTK) pathway; adding to a first well a proposed therapeutic compound that affects an RTK pathway and an RTK stimulating factor for the RTK pathway to form a test well, and adding to another well the RTK stimulating factor to form a control well; continuously monitoring cell-substrate impedance of the at least two wells; and determining a difference in impedance or optionally in cell index between the test well and control well; and if significantly different, concluding the proposed therapeutic compound is therapeutically active in the RTK pathway within the cancer cells of the human subject.

公开(公告)号：US20170205391A1

公开(公告)日：2017-07-20

申请号：US15478041

申请日：2017-04-03

Applicant: ACEA Biosciences, Inc.

Inventor： Nan Li ,  Xiaobo Wang ,  Yama A. Abassi ,  Biao Xi ,  Wen Fu Zhang ,  Xiao Xu

IPC: G01N33/487 ,  G01N33/50

CPC classification number: G01N33/48728 ,  G01N33/4836 ,  G01N33/5008 ,  G01N33/5014 ,  G01N33/5061 ,  G01N33/5073 ,  G01N2800/326 ,  G01N2800/52

Abstract: A method of determining a beating parameter of cells that undergo excitation contraction coupling, the method including providing a cell analysis device having a substrate and a sensor that measures cell adhesion or attachment to the substrate in millisecond time resolution; adding excitable cells capable of undergoing excitation contraction coupling to the substrate; monitoring cell adhesion or attachment of the excitable cells to the substrate in millisecond time resolution; and calculating one or more beating parameters from the monitored adhesion.

公开(公告)号：US09464089B2

公开(公告)日：2016-10-11

申请号：US14329890

申请日：2014-07-11

Applicant: ACEA BIOSCIENCES INC.

Inventor： Xiao Xu ,  Xiaobo Wang ,  Long Mao ,  Li Zhao ,  Biao Xi

IPC: A61K31/506 ,  C07D241/04 ,  C07D239/30 ,  C07D487/04 ,  C07D239/52 ,  C07D239/49 ,  C07D403/12 ,  C07D401/12 ,  C07D473/18 ,  C07D417/12

CPC classification number: C07D519/00 ,  C07D239/47 ,  C07D239/48 ,  C07D239/49 ,  C07D239/52 ,  C07D401/12 ,  C07D403/12 ,  C07D417/12 ,  C07D473/18 ,  C07D487/04

Abstract: The present invention relates to pharmaceutical compounds, compositions and methods, especially as they are related to compositions and methods for the treatment and/or prevention of a proliferation disorder, a cancer, a tumor, an inflammatory disease, an autoimmune disease, psoriasis, dry eye or an immunologically related disease, and in some embodiments diseases or disorders related to the dysregulation of kinase such as, but not limited to, EGFR (including HER), Alk, PDGFR, BLK, BMX/ETK, FLT3(D835Y), ITK, TEC, TXK, BTK, or JAK, and the respective pathways.

Abstract translation: 本发明涉及药物化合物，组合物和方法，特别是因为它们与用于治疗和/或预防增殖病症，癌症，肿瘤，炎性疾病，自身免疫性疾病，牛皮癣，干性疾病的组合物和方法有关 眼睛或免疫相关疾病，在一些实施方案中，与激酶失调相关的疾病或病症，例如但不限于EGFR（包括HER），Alk，PDGFR，BLK，BMX / ETK，FLT3（D835Y），ITK ，TEC，TXK，BTK或JAK，以及各自的途径。

公开(公告)号：US09034885B2

公开(公告)日：2015-05-19

申请号：US13843554

申请日：2013-03-15

Applicant: ACEA BIOSCIENCES INC.

Inventor： Xiao Xu ,  Xiaobo Wang ,  Long Mao ,  Li Zhao ,  Biao Xi

IPC: A01N43/90 ,  A61K31/519 ,  C07D487/04 ,  A61K45/06

CPC classification number: A61K31/519 ,  A61K45/06 ,  C07D487/04 ,  A61K2300/00

Abstract: The present invention relates to certain pyrrolopyrimidine derivatives, pharmaceutical compositions containing them, and methods of using them, including methods for the treatment of tumors and related diseases related to the dysregulation of kinase (such as EGFR (including HER), Alk, PDGFR, but not limited to) pathways.

Abstract translation: 本发明涉及某些吡咯并嘧啶衍生物，含有它们的药物组合物及其使用方法，包括治疗肿瘤的方法和与激酶失调相关的相关疾病（如EGFR（包括HER），Alk，PDGFR），但是 途径。

公开(公告)号：US20140203818A1

公开(公告)日：2014-07-24

申请号：US14092927

申请日：2013-11-28

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Wei Ouyang ,  Nan Li ,  Tianxing Wang ,  Xiaoyu Zhang ,  Xiao Xu ,  Yama A. Abassi

IPC: G01N27/02

CPC classification number: G01N27/02 ,  G01N33/4836

Abstract: Devices, systems and methods for monitoring excitable cells, such as cardiomyocytes, on microelectrode arrays that couple the electro-stimulation of excitable cells to induce or regulate cardiomyocyte beating and the simultaneous measurement of impedance and extracellular recording to assess changes in cardiomyocyte beating, viability, morphology or electrophysical properties in response to a plurality of treatments.

Abstract translation: 用于监测微电极阵列上的可兴奋细胞（例如心肌细胞）的装置，系统和方法，其耦合可刺激细胞的电刺激以诱导或调节心肌细胞搏动，以及同时测量阻抗和细胞外记录以评估心肌细胞跳动，活力， 响应于多种处理的形态或电物理性质。