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21.发明授权Production of fatty alcohols with fatty alcohol forming acyl-CoA reductases (FAR) 有权用脂肪醇形成酰基辅酶A还原酶（FAR）生产脂肪醇公开(公告)号：US08216815B2
公开(公告)日：2012-07-10
申请号：US12825939
申请日：2010-06-29
Applicant: Robert McDaniel , Behnaz Behrouzian , Louis Clark , Douglas Hattendorf , Fernando Valle
Inventor： Robert McDaniel , Behnaz Behrouzian , Louis Clark , Douglas Hattendorf , Fernando Valle
IPC: C12P7/42 , C12P21/06 , C12P21/04 , C12Q1/68 , C12Q1/26 , C12N9/02 , C12N1/20 , C12N15/00 , C07H21/04
CPC classification number: C12P7/04 , C10L1/026 , C10L1/04 , C10L1/19 , C12N9/0008 , C12P7/649 , Y02E50/13
Abstract: The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.
Abstract translation: 本公开涉及从包含编码异源脂肪酰辅酶A还原酶（FAR）酶的基因的重组宿主细胞产生脂肪醇的方法。 本公开还涉及来自海洋细菌，特别是海洋γ蛋白杆菌如马里他杆菌和海洋杆菌的FAR酶及其功能片段; 编码FAR酶的多核苷酸和载体以及包含其的宿主细胞。
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22.发明申请公开(公告)号：US20120088271A1
公开(公告)日：2012-04-12
申请号：US13249219
申请日：2011-09-29
Applicant: Farzad Haerizadeh , Fernando Valle , Guillaume Cottarel
Inventor： Farzad Haerizadeh , Fernando Valle , Guillaume Cottarel
Abstract: Methods and compositions for making stable recombinant yeast 2 μm plasmids are provided. Homologous recombination is performed to clone a nucleic acid of interest into the yeast 2 μm plasmid. Heterologous nucleic acid subsequences are recombined between an FLP and a REP2 gene of the plasmid.
Abstract translation: 提供了制备稳定的重组酵母2μm质粒的方法和组合物。 进行同源重组以将目标核酸克隆到酵母2μm质粒中。 异源核酸子序列在质粒的FLP和REP2基因之间重组。
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23.发明申请Metabolically Engineered Bacterial Strains Having Non-Functional Endogenous Gluconate Transporters 审中-公开具有非功能性内源葡萄糖酸盐转运蛋白的代谢工程细菌菌株公开(公告)号：US20120009635A1
公开(公告)日：2012-01-12
申请号：US13228833
申请日：2011-09-09
Applicant: Timothy C. Dodge , Manoj Kumar , M. Harunur Rashid , Fernando Valle
Inventor： Timothy C. Dodge , Manoj Kumar , M. Harunur Rashid , Fernando Valle
Abstract: The present invention relates to engineering metabolic pathways in bacterial host cells which results in enhanced carbon flow for the production of ascorbic acid (ASA) intermediates. In particular, the invention relates to increasing the production of ASA intermediates in bacterial cells by enhancing the availability of gluconate resulting from the inactivation of endogenous gluconate transporter genes.
Abstract translation: 本发明涉及在细菌宿主细胞中工程代谢途径，其导致用于生产抗坏血酸（ASA）中间体的增强的碳流。 特别地，本发明涉及通过增加由内源性葡萄糖酸转运蛋白基因失活产生的葡萄糖酸盐的可利用性来增加细菌细胞中ASA中间体的产生。
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公开(公告)号：US20110237769A1
公开(公告)日：2011-09-29
申请号：US12459399
申请日：2009-06-30
Applicant: Frank J. Feher , Gregory M. Whited , Gopal K. Chotani , Fernando Valle , Carol Fioresi , Karl J. Sanford , Joseph C. McAuliffe , Marguerite Cervin , Aaron S. Puhala , Andrei Miasnikov , Ilana S. Aldor
Inventor： Frank J. Feher , Gregory M. Whited , Gopal K. Chotani , Fernando Valle , Carol Fioresi , Karl J. Sanford , Joseph C. McAuliffe , Marguerite Cervin , Aaron S. Puhala , Andrei Miasnikov , Ilana S. Aldor
IPC: C08F36/08 , C08F236/08 , C07C11/12 , G01N9/00
CPC classification number: C08F236/18 , C08F2/00 , C08F36/04 , C08F136/08 , C08F236/08 , C08F236/10 , C08F236/12 , C08L9/00 , C12P5/007 , Y10T436/24
Abstract: It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. In some cases, these cells are cultured in a culture medium that includes a carbon source, such as, but not limited to, a carbohydrate, glycerol, glycerine, dihydroxyacetone, one-carbon source, oil, animal fat, animal oil, fatty acid, lipid, phospholipid, glycerolipid, monoglyceride, diglyceride, triglyceride, renewable carbon source, polypeptide (e.g., a microbial or plant protein or peptide), yeast extract, component from a yeast extract, or any combination of two or more of the foregoing. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. It is anticipated that there will be a significant demand for synthetic rubber and other isoprene containing polymers that are synthesized using isoprene of this type which is made from renewable, non-petrochemical based resources. In fact, it is believed that industrial customers and consumers would prefer to purchase isoprene containing polymers that are derived from such environmentally friendly sources to those that are made with isoprene derived from a petrochemical process. It is further believed that customers would be willing to pay premium prices for such environmentally friendly products that are made with renewable resources. However, it is important to be able to verify that such isoprene containing polymers are actually made from non-petrochemical based resources. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ13C value of greater than −22‰. This type of polyisoprene can be a cis-1,4-polyisoprene homopolymer rubber.
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25.发明申请PRODUCTION OF FATTY ALCOHOLS WITH FATTY ALCOHOL FORMING ACYL-COA REDUCTASES (FAR) 有权生产脂肪醇与脂肪醇形成ACYA-COA还原剂（FAR）公开(公告)号：US20110000125A1
公开(公告)日：2011-01-06
申请号：US12825939
申请日：2010-06-29
Applicant: Robert McDaniel , Behnaz Behrouzian , Louis Clark , Douglas Hattendorf , Fernando Valle
Inventor： Robert McDaniel , Behnaz Behrouzian , Louis Clark , Douglas Hattendorf , Fernando Valle
IPC: C10L1/19 , C12P7/64 , C07H21/00 , C12N15/63 , C12N1/21 , C12N1/19 , C12N9/02 , C12P5/00 , C07C9/00 , C09K3/00
CPC classification number: C12P7/04 , C10L1/026 , C10L1/04 , C10L1/19 , C12N9/0008 , C12P7/649 , Y02E50/13
Abstract: The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.
Abstract translation: 本公开涉及从包含编码异源脂肪酰辅酶A还原酶（FAR）酶的基因的重组宿主细胞产生脂肪醇的方法。 本公开还涉及来自海洋细菌，特别是海洋γ蛋白杆菌如马里他杆菌和海洋杆菌的FAR酶及其功能片段; 编码FAR酶的多核苷酸和载体以及包含其的宿主细胞。
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Patent Agency Ranking
公开(公告)号：US07407780B2
公开(公告)日：2008-08-05
申请号：US11598375
申请日：2006-11-13
Applicant: Timothy C. Dodge , Fernando Valle
Inventor： Timothy C. Dodge , Fernando Valle
CPC classification number: C12N9/1205 , C12N15/52 , C12P7/60 , C12P19/02 , C12P25/00
Abstract: The invention provides methods for producing products comprising improved host cells genetically engineered to have uncoupled productive and catabolic pathways. In particular, the present invention provides host cells having a modification in nucleic acid encoding an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a modification of nucleic acid encoding an enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such improved host cells are used for the production of products, such as, ascorbic acid intermediates. Methods for making and using the improved host cells are provided. Nucleic acid and amino acid sequences for glucokinase and gluconokinase are provided.
Abstract translation: 本发明提供了用于生产包含经遗传工程改良的宿主细胞以产生解偶联生产和分解代谢途径的产物的方法。 特别地，本发明提供了在编码在其第六个碳上磷酸化D-葡萄糖的内源酶活性的核酸修饰的宿主细胞和/或编码在其第六碳上磷酸化D-葡萄糖酸的酶活性的核酸的修饰 。 这种改良的宿主细胞用于生产产品，如抗坏血酸中间体。 提供了制备和使用改进的宿主细胞的方法。 提供了用于葡萄糖激酶和葡糖激酶的核酸和氨基酸序列。
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27.发明授权公开(公告)号：US07371558B2
公开(公告)日：2008-05-13
申请号：US10680286
申请日：2003-10-06
Applicant: Marguerite A. Cervin , Phillipe Soucaille , Fernando Valle
Inventor： Marguerite A. Cervin , Phillipe Soucaille , Fernando Valle
Abstract: The present invention provides a microorganism useful for biologically producing 1,3-propanediol from a fermentable carbon source at higher yield than was previously known. The complexity of the cofactor requirements necessitates the use of a whole cell catalyst for an industrial process that utilizes this reaction sequence to produce 1,3-propanediol. The invention provides a microorganism with disruptions in specified genes and alterations in the expression levels of specified genes that is useful in a higher yielding process to produce 1,3-propanediol.
Abstract translation: 本发明提供了一种可用于从可发酵碳源生产1,3-丙二醇的微生物，其产率高于之前已知的。 辅助因子要求的复杂性需要使用全细胞催化剂用于工业过程，其利用该反应顺序产生1,3-丙二醇。 本发明提供了一种在特定基因中具有破坏的微生物，并且在用于产生1,3-丙二醇的更高产率的方法中有用的特定基因的表达水平的改变。
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公开(公告)号：US20070298474A1
公开(公告)日：2007-12-27
申请号：US11879260
申请日：2007-07-16
Applicant: Manoj Kumar , Fernando Valle , Veronique Dartois , James Hoch
Inventor： Manoj Kumar , Fernando Valle , Veronique Dartois , James Hoch
IPC: C12P7/02
CPC classification number: C12P7/60
Abstract: A method for enhancing a host cell's biosynthetic production of 2-KLG is described. Such method comprises selecting a host cell that has an at least partially intracellular synthetic pathway which utilizes 2,5-DKG to produce 2-KLG; increasing the transport of said 2,5-DKG into said host cell while maintaining the integrity of the host cell; culturing the host cell to produce said 2,5-DKG; and producing 2-KLG. The transport of the 2,5-DKG is increased by transforming into the host cell DNA encoding for one or more enzymes transporting the 2,5-DKG into the host cell.
Abstract translation: 描述了增强宿主细胞2-KLG生物合成生产的方法。 这种方法包括选择具有至少部分细胞内合成途径的宿主细胞，其利用2,5-DKG产生2-KLG; 增加所述2,5-DKG进入所述宿主细胞的同时保持宿主细胞的完整性; 培养宿主细胞以产生所述2,5-DKG; 并生产2-KLG。 通过将编码一种或多种将2,5-DKG转运到宿主细胞的酶的宿主细胞DNA转化成2,5-DKG的转运来增加。
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29.发明授权Method of uncoupling the catabolic pathway of glycolysis from the oxidative membrane bound pathway of glucose conversion 有权将糖酵解的分解代谢途径与葡萄糖转化的氧化膜结合途径解偶联的方法公开(公告)号：US07241587B2
公开(公告)日：2007-07-10
申请号：US10117283
申请日：2002-04-04
Applicant: Timothy C. Dodge , Fernando Valle
Inventor： Timothy C. Dodge , Fernando Valle
CPC classification number: C12N9/1205 , C12N15/52 , C12P7/60 , C12P19/02 , C12P25/00
Abstract: The invention provides methods for producing products comprising improved host cells genetically engineered to have uncoupled productive and catabolic pathways. In particular, the present invention provides host cells having a modification in nucleic acid encoding an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a modification of nucleic acid encoding an enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such improved host cells are used for the production of products, such as, ascorbic acid intermediates. Methods for making and using the improved host cells are provided. Nucleic acid and amino acid sequences for glucokinase and gluconokinase are provided.
Abstract translation: 本发明提供了用于生产包含经遗传工程改良的宿主细胞以产生解偶联生产和分解代谢途径的产物的方法。 特别地，本发明提供了在编码在其第六个碳上磷酸化D-葡萄糖的内源酶活性的核酸修饰的宿主细胞和/或编码在其第六碳上磷酸化D-葡萄糖酸的酶活性的核酸的修饰 。 这种改良的宿主细胞用于生产产品，如抗坏血酸中间体。 提供了制备和使用改进的宿主细胞的方法。 提供了用于葡萄糖激酶和葡糖激酶的核酸和氨基酸序列。
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公开(公告)号：US06509185B1
公开(公告)日：2003-01-21
申请号：US09479494
申请日：2000-01-07
Applicant: Fernando Valle , Eugenio Ferrari
Inventor： Fernando Valle , Eugenio Ferrari
IPC: C12N120
CPC classification number: C12N9/54
Abstract: The present invention provides a mutant aprE promoter and methods for the production of a desired protein in a Bacillus host cell, which comprises the mutant aprE promoter. The present invention provides the sequence of a preferred aprE mutant promoter, which provided for a 100-fold increase in the production of a protein from Bacillus subtilis.
Abstract translation: 本发明提供突变体aprE启动子和在芽孢杆菌宿主细胞中产生所需蛋白质的方法，其包含突变体aprE启动子。 本发明提供了优选的aprE突变启动子的序列，其提供了从枯草芽孢杆菌产生蛋白质的100倍的增加。
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