公开(公告)号：US10215639B2

公开(公告)日：2019-02-26

申请号：US15254855

申请日：2016-09-01

Applicant: Massachusetts Institute of Technology

Inventor： Erika Ye ,  Amir H. Atabaki ,  Ningren Han ,  Rajeev Jagga Ram ,  William F. Herrington

IPC: G01J3/28 ,  G01J3/453 ,  G01J3/02

Abstract: A non-paraxial Talbot spectrometer includes a transmission grating to receive incident light. The grating period of the transmission grating is comparable to the wavelength of interest so as to allow the Talbot spectrometer to operate outside the paraxial limit. Light transmitted through the transmission grating forms periodic Talbot images. A tilted detector is employed to simultaneously sample the Talbot images at various distances along a direction perpendicular to the grating. Spectral information of the incident light can be calculated by taking Fourier transform of the measured Talbot images or by comparing the measured Talbot images with a library of intensity patterns acquired with light sources having known wavelengths.

公开(公告)号：US20170107473A1

公开(公告)日：2017-04-20

申请号：US15161112

申请日：2016-05-20

Applicant: Massachusetts Institute of Technology ,  Sanofi

Inventor： Shireen Goh ,  Rajeev Jagga Ram ,  Michelangelo Canzoneri ,  Horst Blum

IPC: C12M1/34 ,  C12M1/00 ,  B01L3/00 ,  C12M1/04

CPC classification number: C12M41/34 ,  B01L3/502723 ,  C12M23/24 ,  C12M23/26 ,  C12M23/34 ,  C12M29/04 ,  C12M41/26 ,  C12M41/32 ,  C12M41/44

Abstract: Strategies to control the level of dissolved carbon dioxide (CO2) concentrations and/or pH in small volume reactor chambers, and associated articles, systems, and methods, are generally provided. In certain embodiments, the reactor chambers can be configured to contain at least one biological cell.

公开(公告)号：US20150132845A1

公开(公告)日：2015-05-14

申请号：US14535048

申请日：2014-11-06

Applicant: Massachusetts Institute of Technology

Inventor： Rajeev Jagga Ram ,  Kevin Shao-Kwan Lee

IPC: C12M1/00 ,  C12M1/34 ,  C12M1/04

CPC classification number: C12M23/26 ,  C12M23/24 ,  C12M29/18 ,  C12M41/40

Abstract: Devices, systems, and methods for continuous cell culture and other reactions are generally described. In some embodiments, chambers (e.g., cell growth chambers) including at least a portion of a wall formed of a flexible member are provided. A retaining structure can be incorporated outside and proximate to the chamber such that when liquid is added to the chamber, the flexible member is consistently and predictably deformed, and a consistent volume of liquid is added. The flexible member can be formed of, in some embodiments, a gas-permeable medium. In some embodiments, reaction chambers can be arranged in a fluidic loop, and a bypass channel can be used to introduce and/or extract fluid from the loop without affecting loop operation.

Abstract translation: 通常描述用于连续细胞培养和其它反应的装置，系统和方法。 在一些实施例中，提供包括由柔性构件形成的壁的至少一部分的室（例如，细胞生长室）。 保持结构可以结合到室的外部和附近，使得当将液体添加到室中时，柔性构件一致地且可预测地变形，并且添加一定体积的液体。 在一些实施例中，柔性构件可由透气性介质形成。 在一些实施例中，反应室可以布置在流体循环中，并且可以使用旁路通道来引入和/或从循环中提取流体而不影响循环操作。