公开(公告)号：US20160160198A1

公开(公告)日：2016-06-09

申请号：US15048624

申请日：2016-02-19

Applicant: General Electric Company

Inventor： John Richard Nelson ,  Robert Scott Duthie ,  Gregory Andrew Grossman ,  Anuradha Sekher

IPC: C12N9/22 ,  C12Q1/68

CPC classification number: C12N9/22 ,  C07K1/00 ,  C07K14/00 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6858 ,  C12Y301/26 ,  C12Q2521/301 ,  C12Q2525/101 ,  C12Q2537/1376 ,  C12Q2527/101

Abstract: Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.

Abstract translation: 本文提供了能够切割含有肌苷的DNA序列的突变体内切核酸酶V酶。 还描述了使用这种突变体内切核酸酶V酶将切口引入到包括一种或多种肌苷的靶DNA中并使用DNA聚合酶产生靶DNA扩增子的核酸测定法和试剂。

公开(公告)号：US20150167065A1

公开(公告)日：2015-06-18

申请号：US14106264

申请日：2013-12-13

Applicant: General Electric Company

Inventor： John Richard Nelson ,  David Roger Moore ,  Bing Li ,  Robert Scott Duthie ,  Patrick McCoy Spooner

IPC: C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q1/6853 ,  C12Q2521/307 ,  C12Q2531/119

Abstract: Provided herein are methods for amplification a target dsDNA that is impregnated within a porous matrix using endonuclease-assisted DNA amplification. The amplicons may be subsequent detected within the porous matrix or may be eluted out of the porous matrix. Methods for extracting a genetic material from a biological sample using endonuclease-assisted DNA amplification within a porous matrix are also provided.

Abstract translation: 本文提供了使用内切核酸酶辅助的DNA扩增来扩增浸渍在多孔基质内的靶dsDNA的方法。 可以在多孔基质内随后检测扩增子，或者可以从多孔基质中洗脱。 还提供了在多孔基质内使用内切核酸酶辅助的DNA扩增从生物样品中提取遗传物质的方法。

公开(公告)号：US20130130352A1

公开(公告)日：2013-05-23

申请号：US13729566

申请日：2012-12-28

Applicant: GENERAL ELECTRIC COMPANY

Inventor： John Richard Nelson ,  Robert Scott Duthie ,  Gregory Andrew Grossman

IPC: C12N9/12

CPC classification number: C12N9/1241 ,  C12Q1/6848 ,  C12Q2525/125 ,  C12Q2521/319

Abstract: Methods and kits for generating contamination-free reagents and reagent solutions for use in nucleic acid amplification are provided. Methods include processing of polymerase solutions, nucleotide solutions and primer solutions to render contaminating nucleic acid inert. The methods employ the proofreading activity of the polymerase and/or exonucleases to de-contaminate the reagents and reagent solutions. Methods and kits for contamination-free nucleic acid amplification are provided.