公开(公告)号：US20170145461A1

公开(公告)日：2017-05-25

申请号：US15342623

申请日：2016-11-03

Applicant: DSM IP Assets B.V.

Inventor： Kirk E. APT ,  James Casey LIPPMEIER ,  David SIMPSON ,  Jun WANG ,  James P. WYNN ,  Ross ZIRKLE

IPC: C12P21/00 ,  C12R1/90 ,  C12N9/26 ,  C12N15/80

CPC classification number: C12P21/00 ,  C07K14/37 ,  C07K14/705 ,  C12N9/2431 ,  C12N15/79 ,  C12N15/80 ,  C12P21/005 ,  C12R1/90 ,  C12Y302/01026

Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.

公开(公告)号：US20170088841A1

公开(公告)日：2017-03-30

申请号：US15371410

申请日：2016-12-07

Applicant: DSM IP Assets B.V.

Inventor： Kirk E. APT ,  Leslie Richter ,  David Simpson ,  Ross Zirkle

IPC: C12N15/52 ,  C12N9/88 ,  C12N9/04 ,  C12P7/64 ,  C12N9/10

CPC classification number: C12N15/52 ,  A61K38/00 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472 ,  C12Y101/01 ,  C12Y103/01 ,  C12Y203/01039 ,  C12Y203/01041 ,  C12Y402/01 ,  C12Y402/01059 ,  Y02P20/52

Abstract: The present invention is directed to isolated nucleic acid molecules and polypeptides of thraustochytrid polyunsaturated fatty acid (PUFA) synthases involved in the production of PUFAs, including PUFAs enriched in docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), or a combination thereof. The present invention is directed to vectors and host cells comprising the nucleic acid molecules, polypeptides encoded by the nucleic acid molecules, compositions comprising the nucleic acid molecules or polypeptides, and methods of making and uses thereof.

公开(公告)号：US20150223492A1

公开(公告)日：2015-08-13

申请号：US14698378

申请日：2015-04-28

Applicant: DSM IP Assets B.V.

Inventor： Joseph W. PFEIFER, III ,  Jon Milton HANSEN ,  Jose R. GARCIA ,  Xiao Daniel DONG ,  Paul Warren BEHRENS ,  Kirk E. APT

IPC: A23K1/00 ,  A23K1/16 ,  A23K1/18 ,  C12P7/64

CPC classification number: A23K50/00 ,  A23K10/16 ,  A23K20/158 ,  A23K50/80 ,  C11B1/10 ,  C12N1/12 ,  C12P7/6427 ,  C12R1/89

Abstract: The present invention is directed to isolated microorganisms as well as strains and mutants thereof, biomasses, microbial oils, compositions, and cultures; methods of producing the microbial oils, biomasses, and mutants; and methods of using the isolated microorganisms, biomasses, and microbial oils.

公开(公告)号：US20150152450A1

公开(公告)日：2015-06-04

申请号：US14566458

申请日：2014-12-10

Applicant: DSM IP ASSETS B.V.

Inventor： Kirk E. APT ,  Leslie RICHTER ,  David SIMPSON ,  Ross ZIRKLE

IPC: C12P7/64 ,  C12N9/02 ,  C12N9/88

CPC classification number: C12N15/52 ,  A61K38/00 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472 ,  C12Y101/01 ,  C12Y103/01 ,  C12Y203/01039 ,  C12Y203/01041 ,  C12Y402/01 ,  C12Y402/01059 ,  Y02P20/52

Abstract: The present invention is directed to isolated nucleic acid molecules and polypeptides of thraustochytrid polyunsaturated fatty acid (PUFA) synthases involved in the production of PUFAs, including PUFAs enriched in docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), or a combination thereof. The present invention is directed to vectors and host cells comprising the nucleic acid molecules, polypeptides encoded by the nucleic acid molecules, compositions comprising the nucleic acid molecules or polypeptides, and methods of making and uses thereof.

Abstract translation: 本发明涉及涉及PUFA生产的分离的核酸分子和多烯突变多不饱和脂肪酸（PUFA）合成酶，包括富含二十二碳六烯酸（DHA），二十碳五烯酸（EPA）或其组合的PUFA。 本发明涉及包含核酸分子，核酸分子编码的多肽，包含核酸分子或多肽的组合物及其制备和用途的载体和宿主细胞。

公开(公告)号：US20140106402A1

公开(公告)日：2014-04-17

申请号：US14108794

申请日：2013-12-17

Applicant: DSM IP Assets B.V.

Inventor： Kirk E. APT ,  James Casey LIPPMEIER ,  David SIMPSON ,  Jun WANG ,  James P. WYNN ,  Ross ZIRKLE

IPC: C12P21/00 ,  C12R1/90

CPC classification number: C12P21/00 ,  C07K14/37 ,  C07K14/705 ,  C12N9/2431 ,  C12N15/79 ,  C12N15/80 ,  C12P21/005 ,  C12R1/90 ,  C12Y302/01026

Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.

Abstract translation: 本发明涉及神经胶霉病菌门的重组细胞和微生物及其在异源蛋白质生产中的用途。 用于有效生产和任选地从重组宿主细胞和微生物分泌多肽的新型启动子，终止子和信号序列也包括在本发明中。