公开(公告)号：US08921539B2

公开(公告)日：2014-12-30

申请号：US13434656

申请日：2012-03-29

申请人： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde

发明人： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde

IPC分类号： C07H21/00

CPC分类号： C12N15/101

摘要： The invention provides pipette tip columns and automated methods for the purification of nucleic acids such as plasmids from unclarified cell lysates containing cell debris as well as methods for making and using such columns. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit.

摘要翻译： 本发明提供了用于纯化核酸例如来自含有细胞碎片的未澄清细胞裂解物的质粒的移液管尖塔和自动化方法，以及用于制备和使用这些柱的方法。 柱通常包括位于移液管末端柱中的介质床，位于底部玻璃料上方并具有可选的顶部玻璃料。

公开(公告)号：US08664378B2

公开(公告)日：2014-03-04

申请号：US13250040

申请日：2011-09-30

申请人： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde

发明人： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde

IPC分类号： C07H21/00

摘要： The present invention solves the problem of isolating nucleic acids from cells in the presence of the growth medium. The invention is particularly useful for isolating extrachromosomal replicons such as plasmids. Cells are lysed in the presence of the medium in which they were grown and nucleic acids are isolated using a pipette tip column. A liquid handling robot can be used to isolate nucleic acids from multiple samples simultaneously without the need for human intervention.

公开(公告)号：US20120083598A1

公开(公告)日：2012-04-05

申请号：US13250040

申请日：2011-09-30

申请人： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde

发明人： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde

IPC分类号： C07H21/00

CPC分类号： C12N15/1017

摘要： The present invention solves the problem of isolating nucleic acids from cells in the presence of the growth medium. The invention is particularly useful for isolating extrachromosomal replicons such as plasmids. Cells are lysed in the presence of the medium in which they were grown and nucleic acids are isolated using a pipette tip column. A liquid handling robot can be used to isolate nucleic acids from multiple samples simultaneously without the need for human intervention.

摘要翻译： 本发明解决了在生长培养基存在下从细胞中分离核酸的问题。 本发明特别可用于分离染色体外复制子如质粒。 细胞在其生长培养基的存在下裂解，并使用移液管末端柱分离核酸。 可以使用液体处理机器人同时分离多个样品中的核酸而不需要人为干预。

公开(公告)号：US20100200509A1

公开(公告)日：2010-08-12

申请号：US12767659

申请日：2010-04-26

申请人： Chris Suh ,  Lee Hoang ,  Jennifer E. Grant ,  Douglas T. Gjerde

发明人： Chris Suh ,  Lee Hoang ,  Jennifer E. Grant ,  Douglas T. Gjerde

IPC分类号： B01D15/04

CPC分类号： C12N15/101 ,  B01J20/285 ,  B01J20/286 ,  B01J20/291 ,  B01J20/3244 ,  B01J2220/54 ,  B01J2220/58 ,  B01J2220/64 ,  B82Y30/00 ,  G01N1/405 ,  G01N30/02 ,  G01N30/603 ,  G01N30/6065 ,  G01N2035/1053 ,  B01D15/3804

摘要： The invention provides pipette tip extraction columns for the purification of a DNA vector from un-clarified cell lysate containing cell debris as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the pipette tip column, above a bottom frit and with an optional top frit.

摘要翻译： 本发明提供了用于从含有细胞碎片的未澄清细胞裂解物中纯化DNA载体的移液管尖端提取柱以及用于制备和使用这些柱的方法。 柱通常包括位于移液管末端柱中的提取介质床，位于底部玻璃料上方并具有可选的顶部玻璃料。