公开(公告)号：US09416393B2

公开(公告)日：2016-08-16

申请号：US14512708

申请日：2014-10-13

Applicant: American Sterilizer Company

Inventor： Phillip P. Franciskovich ,  Tricia A. Cregger ,  William A. Yirava ,  Peter A. Burke

IPC: C12Q1/22 ,  C12Q1/34 ,  A61L2/28 ,  C12M1/34 ,  C12Q1/68

CPC classification number: C12N15/75 ,  A61L2/28 ,  C12M1/34 ,  C12M37/06 ,  C12N9/2471 ,  C12Q1/22 ,  C12Q1/34 ,  C12Q1/689 ,  C12Q1/6897 ,  C12Y302/01023

Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.

Abstract translation: 本发明涉及一种衍生自组合物的生物指示剂，其包含：宿主生物，其包含孢子形成细菌; 用于产生指示酶的报告基因; 调节基因; 以及用于将报道基因和调节基因插入宿主生物体的载体; 宿主生物体在其基因组中具有转座遗传元件，用于将插入序列插入到调节基因中; 所述插入序列包含转座酶，一对末端反向重复序列和用于表达转座酶的至少一个开放阅读框。 载体可能被宿主生物体吸收。 插入序列可插入调节基因。 宿主生物体可能经历孢子形成以形成生物指示剂。 公开了一种使用生物指示剂的方法和装置。