知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

22 条记录 (耗时 0.031 秒)

    Secretion factors for gram-positive microorganisms genes encoding them and methods of using it
    13.
    发明授权
    Secretion factors for gram-positive microorganisms genes encoding them and methods of using it 失效
    编码革兰氏阳性微生物基因的分泌因子及其使用方法

    公开(公告)号：US06410262B1

    公开(公告)日：2002-06-25

    申请号：US08981527

    申请日：1997-12-16

    申请人： Wilhelmus J. Quax ,  Richard Kerkman ,  Cornelis P. Broekhuizen

    发明人： Wilhelmus J. Quax ,  Richard Kerkman ,  Cornelis P. Broekhuizen

    IPC分类号： C12P2106

    CPC分类号： C07K14/32 ,  C12P21/02

    摘要： Heterologous protein secretion from Gram-positive bacteria, in particular from Bacilli has, with few exceptions, met with little success. Incompatibility of the heterologous proteins with the protein secretion machinery of the host is the main cause of this effect. This limiting factor for the production of heterologous proteins in commercially significant concentrations from Bacillus subtilis is removed by overexpressing the Bacillus subtilis protein FtsY or FtsY protein in combination with overexpression of other members of the bacterial signal recognition particle. Said gene(s) is(are) overexpressed in Bacillus host cells expressing a heterologous protein which then shows an increased amount of the heterologous protein secreted in the surrounding medium.

    摘要翻译： 来自革兰氏阳性菌，特别是芽孢杆菌的异源蛋白质分泌除了少数例外，几乎没有成功。 异源蛋白与宿主的蛋白质分泌机制的不相容性是造成这种影响的主要原因。 通过过表达枯草芽孢杆菌蛋白FtsY或FtsY蛋白以及细菌信号识别颗粒的其他成员的过表达，可以除去生产来自枯草芽孢杆菌的商业上显着浓度的异源蛋白质的限制因素。 所述基因在表达异源蛋白质的芽孢杆菌宿主细胞中过表达，其然后显示在周围培养基中分泌的异源蛋白质的量增加。

    Expression system for altered expression levels
    14.
    发明授权
    Expression system for altered expression levels 失效
    表达系统改变表达水平

    公开(公告)号：US6048710A

    公开(公告)日：2000-04-11

    申请号：US911853

    申请日：1997-08-15

    申请人： Gijsbert Gerritse ,  Wilhelmus J. Quax

    发明人： Gijsbert Gerritse ,  Wilhelmus J. Quax

    IPC分类号： C07K14/21 ,  C12N9/12 ,  C12N9/20 ,  C12N15/00 ,  C12N15/31 ,  C12N15/54 ,  C12N15/78 ,  C12P21/00 ,  C12N9/00 ,  C12N15/63

    CPC分类号： C07K14/21 ,  C12N15/78 ,  C12N9/20

    摘要： A new expression system is provided which comprises component(s) of a lipase regulation cascade. the lipase regulation cascade as disclosed herein includes a kinase, a DNA binding regulator, a polymerase, a promoter, an upstream activating sequence, and secretion factors. Plasmids and transformed cells are also provided as well as methods of transforming host cells using the plasmids. Further, there is provided a kinase that can regulate the expression of a protein, a DNA binding regulator that can regulate the expression of a protein, a Pseudomonas alcaligenes polymerase, a Pseudomonas alcaligenes sigma 54 promoter, a Pseudomonas alcaligenes upstream activating sequence, the Pseudomonas alcaligenes secretion factors XcpP, XcpQ, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and the xcp regulators OrfV, OrfX.

    摘要翻译： 提供了一种新的表达系统，其包含脂肪酶调节级联的组分。 如本文所公开的脂肪酶调节级联包括激酶，DNA结合调节剂，聚合酶，启动子，上游激活序列和分泌因子。 还提供质粒和转化细胞以及使用质粒转化宿主细胞的方法。 此外，提供了可以调节蛋白质的表达的激酶，可调节蛋白质表达的DNA结合调节剂，产碱假单胞菌聚合酶，产碱假马杆菌54启动子，产碱假单胞菌（Pseudomonas alcaligenes）上游激活序列，假单胞菌 产碱分泌因子XcpP，XcpQ，XcpR，XcpS，XcpT，XcpU，XcpV，XcpW，XcpX，XcpY，XcpZ和xcp调节剂OrfV OrfX。

    Secretion factors for gram-positive micoorganisms, genes encoding them and methods of using it
    20.
    发明授权
    Secretion factors for gram-positive micoorganisms, genes encoding them and methods of using it 失效
    革兰氏阳性微生物的分泌因子，编码它们的基因及其使用方法

    公开(公告)号：US06692951B2

    公开(公告)日：2004-02-17

    申请号：US10147936

    申请日：2002-05-17

    申请人： Wilhelmus J. Quax ,  Richard Kerkman ,  Cornelis P. Broekhuizen

    发明人： Wilhelmus J. Quax ,  Richard Kerkman ,  Cornelis P. Broekhuizen

    IPC分类号： C12N121

    CPC分类号： C07K14/32 ,  C12P21/02

    摘要： Heterologous protein secretion from Gram-positive bacteria, in particular from Bacilli has, with few exceptions, met with little success. Incompatibility of the heterologous proteins with the protein secretion machinery of the host is the main cause of this effect. This limiting factor for the production of heterologous proteins in commercially significant concentrations from Bacillus subtilis is removed by overexpressing the Bacillus subtilis protein FtsY or FtsY protein in combination with overexpression of other members of the bacterial signal recognition particle. Said gene(s) is(are) overexpressed in Bacillus host cells expressing a heterologous protein which then shows an increased amount of the heterologous protein secreted in the surrounding medium.

    摘要翻译： 来自革兰氏阳性菌，特别是芽孢杆菌的异源蛋白质分泌除了少数例外，几乎没有成功。 异源蛋白与宿主的蛋白质分泌机制的不相容性是造成这种影响的主要原因。 通过过表达枯草芽孢杆菌蛋白FtsY或FtsY蛋白以及细菌信号识别颗粒的其他成员的过表达，可以除去生产来自枯草芽孢杆菌的商业上显着浓度的异源蛋白质的限制因素。 所述基因在表达异源蛋白质的芽孢杆菌宿主细胞中过表达，其然后显示在周围培养基中分泌的异源蛋白质的量增加。