公开(公告)号：US20140053095A1

公开(公告)日：2014-02-20

申请号：US14063940

申请日：2013-10-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gordon A. Janaway ,  Evelyn Wing-Sim Chan

IPC: G06F19/26

CPC classification number: G06F19/18 ,  G06F3/0482 ,  G06F3/0485 ,  G06F19/26

Abstract: Systems and methods are used to display data obtained from a qPCR instrument. Each of two or more samples is probed with a first labeling probe and a second labeling probe. A first data set is received from a qPCR instrument at a first cycle number that includes for each sample a first labeling probe intensity, and a second labeling probe intensity. A second data set is received at a second cycle number that includes for each sample a first labeling probe intensity and a second labeling probe intensity. A first plot of first labeling probe intensity as a function of second labeling probe intensity is created using the first data set. A second plot of first labeling probe intensity as a function of second labeling probe intensity is created using the second data set. The first plot and the second plot are displayed in response to user defined input to provide dynamic and real-time analysis of genotyping data.

Abstract translation: 系统和方法用于显示从qPCR仪器获得的数据。 用第一标记探针和第二标记探针探测两个或更多个样品中的每一个。 从qPCR仪器以第一周期数接收第一数据集，其中包括针对每个样本的第一标记探针强度和第二标记探针强度。 以第二周期数接收第二数据集，其中为每个样本包括第一标记探针强度和第二标记探针强度。 使用第一数据集创建第一标记探针强度作为第二标记探针强度的函数的第一图。 使用第二数据集创建第二标记探针强度作为第二标记探针强度的函数的第二图。 显示第一个绘图和第二个绘图以响应用户定义的输入，以提供对基因分型数据的动态和实时分析。

公开(公告)号：US20190130995A1

公开(公告)日：2019-05-02

申请号：US16172330

申请日：2018-10-26

Applicant: Life Technologies Corporation

Inventor： Gordon A. Janaway ,  Evelyn Wing-Sim Chan

IPC: G16B20/00 ,  G16B45/00 ,  G06F3/0482 ,  G06F3/0485

Abstract: Systems and methods are used to display data obtained from a qPCR instrument. Each of two or more samples is probed with a first labeling probe and a second labeling probe. A first data set is received from a qPCR instrument at a first cycle number that includes for each sample a first labeling probe intensity, and a second labeling probe intensity. A second data set is received at a second cycle number that includes for each sample a first labeling probe intensity and a second labeling probe intensity. A first plot of first labeling probe intensity as a function of second labeling probe intensity is created using the first data set. A second plot of first labeling probe intensity as a function of second labeling probe intensity is created using the second data set. The first plot and the second plot are displayed in response to user defined input to provide dynamic and real-time analysis of genotyping data.

公开(公告)号：US09390227B2

公开(公告)日：2016-07-12

申请号：US14063940

申请日：2013-10-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gordon A. Janaway ,  Evelyn Wing-Sim Chan

IPC: G06F3/00 ,  G06F19/26 ,  G06F19/18

CPC classification number: G06F19/18 ,  G06F3/0482 ,  G06F3/0485 ,  G06F19/26

Abstract: Systems and methods are used to display data obtained from a qPCR instrument. Each of two or more samples is probed with a first labeling probe and a second labeling probe. A first data set is received from a qPCR instrument at a first cycle number that includes for each sample a first labeling probe intensity, and a second labeling probe intensity. A second data set is received at a second cycle number that includes for each sample a first labeling probe intensity and a second labeling probe intensity. A first plot of first labeling probe intensity as a function of second labeling probe intensity is created using the first data set. A second plot of first labeling probe intensity as a function of second labeling probe intensity is created using the second data set. The first plot and the second plot are displayed in response to user defined input to provide dynamic and real-time analysis of genotyping data.

公开(公告)号：US20150278437A1

公开(公告)日：2015-10-01

申请号：US14428312

申请日：2013-09-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Nivedita Sumi Majumdar ,  Thomas Wessel ,  David C. Woo ,  Marcin Sikora ,  Gordon A. Janaway ,  Shweta Raizada ,  Joanna Lankester ,  Jeffrey A. Marks ,  Daniel Wessel

IPC: G06F19/20 ,  C12Q1/68

CPC classification number: G16B25/00 ,  C12Q1/686 ,  G16B40/00

Abstract: A computer-implemented method for designing a digital PCR (dPCR) experiment is provided. The method includes receiving, from a user, a selection of optimization type. The optimization type may be maximizing the dynamic range, minimizing the number of substrates including reaction sites needed for the experiment, determining a dilution factor, or determining the lower limit of detection, for example. The method further includes receiving, from the user, a precision measure for an experiment, and a minimum concentration of a target in a reaction site for the experiment. The method also includes determining a set of dPCR experiment design factors for the experiment based on the optimization type. The set of dPCR experiment design factors is then displayed to the user.

Abstract translation: 提供了一种用于设计数字PCR（dPCR）实验的计算机实现方法。 该方法包括从用户接收优化类型的选择。 优化类型可以使动态范围最大化，例如最小化包括实验所需的反应位点，确定稀释因子或确定检测下限的底物数量。 该方法还包括从用户接收实验的精确度量，以及用于实验的反应部位中靶的最小浓度。 该方法还包括基于优化类型确定实验的一组dPCR实验设计因子。 然后将该组dPCR实验设计因子显示给用户。

公开(公告)号：US20140236496A1

公开(公告)日：2014-08-21

申请号：US14347864

申请日：2012-09-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gordon A. Janaway ,  Manjula Aliminati ,  Ruoyun Wu ,  David Fortescue ,  Ming Shen

IPC: G06F19/24

CPC classification number: G16B40/00 ,  C12Q1/6851 ,  C12Q2537/165 ,  C12Q2563/159

Abstract: A computer-implemented method of generating a digital polymerase chain reaction (dPCR) result is provided. The method includes detecting a first set of emission data from a plurality of samples, each included in a sample region of a plurality of sample regions, at a first time during an amplification period. The method further includes determining a positive or negative amplification determination for each sample of the plurality of samples based in part on the first set of emission data. A dPCR result is generated based on the positive or negative amplification determinations for the plurality of samples.

Abstract translation: 提供了产生数字聚合酶链反应（dPCR）结果的计算机实现的方法。 该方法包括在放大期间的第一时间，从多个样本中检测出第一组发射数据，每个采样包括多个采样区域的样本区域。 该方法还包括部分地基于第一组发射数据确定多个样本中的每个样本的正或负放大确定。 基于多个样本的正或负放大确定产生dPCR结果。