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公开(公告)号：US10898589B2
公开(公告)日：2021-01-26
申请号：US15899336
申请日：2018-02-19
申请人： CureVac AG
发明人： Andreas Thess , Thomas Schlake , Jochen Probst
摘要： The present disclosure concerns a nucleic acid sequence, comprising or coding for a coding region, encoding at least one peptide or protein comprising a therapeutic protein or a fragment, variant or derivative thereof, at least one histone stem-loop and a poly(A) sequence or a polyadenylation signal. The present disclosure further concerns a method for increasing the expression of a peptide or protein comprising a therapeutic protein or a fragment, variant or derivative thereof, using the nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal.
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公开(公告)号：US10653799B2
公开(公告)日：2020-05-19
申请号：US15810080
申请日：2017-11-12
申请人： CureVac AG
发明人： Andreas Thess , Thomas Schlake , Jochen Probst
IPC分类号： A61K48/00 , C12N15/67 , C07K14/435 , A61K39/00
摘要： The present application describes a coding nucleic acid sequence, particularly a messenger RNA (mRNA), comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal and the use thereof for increasing the expression of an encoded protein. It also discloses its use for the preparation of a pharmaceutical composition, especially a vaccine e.g. for the use in the treatment of tumours and cancer diseases, cardiovascular diseases, infectious diseases, autoimmune diseases or genetic diseases, or in gene therapy. The present invention further describes an in vitro transcription method, in vitro methods for increasing the expression of a protein using the nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal and an ex vivo and in vivo method.
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公开(公告)号：US20180271964A1
公开(公告)日：2018-09-27
申请号：US16002695
申请日：2018-06-07
申请人： CureVac AG
发明人： Andreas Thess , Thomas Schlake , Jochen Probst
摘要： The present invention relates to a nucleic acid sequence, comprising or coding for a coding region, encoding at least one peptide or protein comprising a tumour antigen or a fragment, variant or derivative thereof, at least one histone stem-loop and a poly(A) sequence or a polyadenylation signal. Furthermore the present invention provides the use of the nucleic acid for increasing the expression of said encoded peptide or protein. It also discloses its use for the preparation of a pharmaceutical composition, especially a vaccine, e.g. for use in the treatment of cancer or tumour diseases. The present invention further describes a method for increasing the expression of a peptide or protein comprising a tumour antigen or a fragment, variant or derivative thereof, using the nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal.
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公开(公告)号：US11345920B2
公开(公告)日：2022-05-31
申请号：US17542430
申请日：2021-12-05
申请人： CureVac AG
发明人： Andreas Thess , Thomas Schlake , Stefanie Grund
IPC分类号： C12N15/67 , A61K39/205 , A61K39/12 , A61K39/145 , C12N15/85 , C12N15/68 , A61K39/00 , A61K48/00
摘要： The invention relates to an artificial nucleic acid molecule comprising an open reading frame and a 3′-UTR comprising at least one poly(A) sequence or a polyadenylation signal. The invention also relates to a method for increasing protein production from an artificial nucleic acid molecule and to the use of a 3′-UTR for a method for increasing protein production from an artificial nucleic acid molecule. Moreover, the invention concerns the use of the artificial nucleic acid molecule as a medicament, as a vaccine or in gene therapy.
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公开(公告)号：US10111968B2
公开(公告)日：2018-10-30
申请号：US15233933
申请日：2016-08-10
申请人： CureVac AG
发明人： Andreas Thess , Thomas Schlake , Jochen Probst
IPC分类号： A61K48/00 , C12N15/63 , C12N15/67 , C12N15/85 , C07K14/505 , C07K16/32 , A61K38/02 , A61K38/03 , A61K38/04 , A61K38/10 , A61K38/18 , C07H21/02
摘要： The present invention relates to a nucleic add sequence, comprising or coding for a coding region, encoding at least one peptide or protein comprising a therapeutic protein or a fragment, variant or derivative thereof, at least one histone stem-loop and a poly(A) sequence or a polyadenylation signal. Furthermore the present invention provides the use of the nucleic add for increasing the expression of the encoded peptide or protein, particularly for the use in gene therapy. It also discloses its use for the preparation of a pharmaceutical composition, e.g., for use in gene therapy, particularly in the treatment of diseases which are in need of a treatment with a therapeutic peptide or protein, preferably as defined herein. The present invention further describes a method for increasing the expression of a peptide or protein comprising a therapeutic protein or a fragment, variant or derivative thereof, using the nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal.
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公开(公告)号：US10010592B2
公开(公告)日：2018-07-03
申请号：US14378572
申请日：2013-02-15
申请人： CureVac AG
发明人： Andreas Thess , Thomas Schlake , Jochen Probst
摘要： The present invention relates to a nucleic acid sequence, comprising or coding for a coding region, encoding at least one peptide or protein comprising a tumor antigen or a fragment, variant or derivative thereof, at least one histone stem-loop and a poly(A) sequence or a polyadenylation signal. Furthermore the present invention provides the use of the nucleic acid for increasing the expression of said encoded peptide or protein. It also discloses its use for the preparation of a pharmaceutical composition, especially a vaccine, e.g. for use in the treatment of cancer or tumor diseases. The present invention further describes a method for increasing the expression of a peptide or protein comprising a tumor antigen or a fragment, variant or derivative thereof, using the nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal.
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公开(公告)号：US09839697B2
公开(公告)日：2017-12-12
申请号：US14945349
申请日：2015-11-18
申请人： CureVac AG
发明人： Andreas Thess , Thomas Schlake , Jochen Probst
IPC分类号： A61K48/00 , C12N15/67 , C07K14/435 , A61K39/00 , A61K38/02 , A61K38/03 , A61K38/04 , A61K38/10 , A61K38/16 , C07H21/04
CPC分类号： A61K48/0066 , A61K39/0011 , A61K2039/53 , A61K2039/575 , C07K14/435 , C12N15/67
摘要： The present application describes a coding nucleic acid sequence, particularly a messenger RNA (mRNA), comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal and the use thereof for increasing the expression of an encoded protein. It also discloses its use for the preparation of a pharmaceutical composition, especially a vaccine e.g. for the use in the treatment of tumors and cancer diseases, cardiovascular diseases, infectious diseases, autoimmune diseases or genetic diseases, or in gene therapy. The present invention further describes an in vitro transcription method, in vitro methods for increasing the expression of a protein using the nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal and an ex vivo and in vivo method.
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公开(公告)号：US11110156B2
公开(公告)日：2021-09-07
申请号：US16002695
申请日：2018-06-07
申请人： CureVac AG
发明人： Andreas Thess , Thomas Schlake , Jochen Probst
摘要： The present invention relates to a nucleic acid sequence, comprising or coding for a coding region, encoding at least one peptide or protein comprising a tumour antigen or a fragment, variant or derivative thereof, at least one histone stem-loop and a poly(A) sequence or a polyadenylation signal. Furthermore the present invention provides the use of the nucleic acid for increasing the expression of said encoded peptide or protein. It also discloses its use for the preparation of a pharmaceutical composition, especially a vaccine, e.g. for use in the treatment of cancer or tumour diseases. The present invention further describes a method for increasing the expression of a peptide or protein comprising a tumour antigen or a fragment, variant or derivative thereof, using the nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal.
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公开(公告)号：US10738306B2
公开(公告)日：2020-08-11
申请号：US15590370
申请日：2017-05-09
申请人： CureVac AG
发明人： Andreas Thess
IPC分类号： C12N15/113 , C12N15/67 , C12N15/85 , A61K39/00 , A61K48/00
摘要： The invention relates to an artificial nucleic acid molecule comprising at least one 5′UTR element which is derived from a TOP gene, at least one open reading frame, and preferably at least one histone stem-loop. Optionally the artificial nucleic acid molecule may further comprise, e.g. a poly(A)sequence, a poyladenylation signal, and/or a 3′UTR. The invention further relates to the use of such an artificial nucleic acid molecule in gene therapy and/or genetic vaccination.
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公开(公告)号：US10047375B2
公开(公告)日：2018-08-14
申请号：US15195934
申请日：2016-06-28
申请人： CureVac AG
发明人： Andreas Thess
IPC分类号： C12P21/02 , C12N15/67 , C12N15/85 , C07H21/02 , C07H21/04 , C12N15/09 , C12N15/63 , C12N15/68 , A61K48/00
摘要： The invention relates to an artificial nucleic acid molecule comprising at least one open reading frame and at least one 3′-untranslated region element (3′-UTR) element comprising a nucleic acid sequence which is derived from the 3′-UTR of a ribosomal protein gene. The invention further relates to the use of such an artificial nucleic acid molecule in gene therapy and/or genetic vaccination. Furthermore, the invention relates to the use of a 3′-UTR element comprising a nucleic acid sequence which is derived from the 3′-UTR of a ribosomal protein gene for enhancing, stabilizing and/or prolonging protein expression from a nucleic acid sequence comprising such 3′-UTR element.
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