公开(公告)号：US20140038215A1

公开(公告)日：2014-02-06

申请号：US13953105

申请日：2013-07-29

Applicant: Agilent Technologies, Inc.

Inventor： Brian Phillip Smart ,  Magdalena Anna Ostrowski ,  Gregory Staples

IPC: C12Q1/34

CPC classification number: C12Q1/34 ,  G01N30/6095 ,  G01N2030/8818 ,  G01N2333/924 ,  G01N2400/12 ,  G01N2400/38

Abstract: A microfluidic device for glycan analysis includes a deglycosylation column comprising a glycosidase attached to a solid support; a tagging column comprising a reactive ester for reaction with an amino group, wherein the tagging column is arranged downstream of the deglycosylation column; an analytical column comprising a stationary phase capable of separating a derivatized glycan; and a plurality of inlet/outlet ports configured to connect with channels on a switching element to form flow paths.

Abstract translation: 用于聚糖分析的微流体装置包括去糖基化柱，其包含连接到固体支持物上的糖苷酶; 标记柱，其包含用于与氨基反应的反应性酯，其中所述标记柱位于去糖基化柱的下游; 包括能够分离衍生的聚糖的固定相的分析柱; 以及多个入口/出口端口，其被配置为与开关元件上的通道连接以形成流动路径。