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公开(公告)号：US20170275631A1
公开(公告)日：2017-09-28
申请号：US15618156
申请日：2017-06-09
Applicant: American Sterilizer Company
Inventor： Phillip P. Franciskovich , Tricia A. Cregger , William A. Yirava , Peter A. Burke
CPC classification number: C12N15/75 , A61L2/28 , C12M1/34 , C12M37/06 , C12N9/2471 , C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Y302/01023
Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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公开(公告)号：US09701968B2
公开(公告)日：2017-07-11
申请号：US15204124
申请日：2016-07-07
Applicant: American Sterilizer Company
Inventor： Phillip P. Franciskovich , Tricia A. Cregger , William A. Yirava , Peter A. Burke
CPC classification number: C12N15/75 , A61L2/28 , C12M1/34 , C12M37/06 , C12N9/2471 , C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Y302/01023
Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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公开(公告)号：US20160312263A1
公开(公告)日：2016-10-27
申请号：US15204124
申请日：2016-07-07
Applicant: American Sterilizer Company
Inventor： Phillip P. Franciskovich , Tricia A. Cregger , William A. Yirava , Peter A. Burke
CPC classification number: C12N15/75 , A61L2/28 , C12M1/34 , C12M37/06 , C12N9/2471 , C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Y302/01023
Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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公开(公告)号：US20160102335A1
公开(公告)日：2016-04-14
申请号：US14512708
申请日：2014-10-13
Applicant: AMERICAN STERILIZER COMPANY
Inventor： Phillip P. Franciskovich , Tricia A. Cregger , William A. Yirava , Peter A. Burke
CPC classification number: C12N15/75 , A61L2/28 , C12M1/34 , C12M37/06 , C12N9/2471 , C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Y302/01023
Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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15.发明申请STERILIZATION INDICATOR INCLUDING A SIMPLIFIED GENETICALLY ENGINEERED BIOLOGICAL INDICATOR 有权灭菌指示剂，包括简化的遗传工程生物指示剂公开(公告)号：US20140273072A1
公开(公告)日：2014-09-18
申请号：US13832019
申请日：2013-03-15
Applicant: AMERICAN STERILIZER COMPANY
Inventor： Phillip P. Franciskovich , Tricia A. Cregger , William A. Yirava
IPC: C12Q1/22
Abstract: A sterilization indicator, including a first compartment containing a genetically engineered biological indicator, and a second compartment containing an enzyme substrate, the second compartment adapted to maintain the enzyme substrate separate from the biological indicator during sterilization, and to permit the enzyme substrate to contact the biological indicator after the biological indicator has been exposed to the sterilization medium; in which the genetically engineered biological indicator comprises at least one test organism and at least one reporter gene suitable for producing an indicator enzyme, the reporter gene being taken up by the test organism; and the test organism is free of any active or activatable repressor gene that would inhibit expression of the reporter gene if present in the test organism or sterilization indicator, and the indicator enzyme and the enzyme substrate are selected such that enzymatic action of the indicator enzyme upon the enzyme substrate yields a detectable signal.
Abstract translation: 灭菌指示剂，包括含有遗传工程生物指示剂的第一隔室和含有酶底物的第二隔室，所述第二隔室适于在灭菌期间将酶底物与生物指示剂分离，并允许酶底物接触 生物指示剂在生物指示剂暴露于灭菌介质之后; 其中遗传工程生物指示剂包含至少一种测试生物体和至少一种适于产生指示酶的报告基因，所述报道基因被测试生物体吸收; 并且测试生物体不含任何活性或可激活的阻遏物基因，如果存在于测试生物或灭菌指示剂中，则抑制报告基因的表达，并且指示酶和酶底物被选择为使指示酶的酶作用 酶底物产生可检测信号。
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Patent Agency Ranking
公开(公告)号：US10301632B2
公开(公告)日：2019-05-28
申请号：US16001266
申请日：2018-06-06
Applicant: American Sterilizer Company
Inventor： Phillip P. Franciskovich , Tricia A. Cregger , William A. Yirava , Peter A. Burke
IPC: C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Q1/68 , C12N15/75 , A61L2/28 , C12M1/34 , C12M1/12 , C12N9/38
Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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公开(公告)号：US20180273958A1
公开(公告)日：2018-09-27
申请号：US16001266
申请日：2018-06-06
Applicant: American Sterilizer Company
Inventor： Phillip P. Franciskovich , Tricia A. Cregger , William A. Yirava , Peter A. Burke
IPC: C12N15/75 , C12Q1/689 , C12M1/34 , C12Q1/34 , C12N9/38 , C12Q1/6897 , C12M1/12 , C12Q1/22 , A61L2/28
CPC classification number: C12N15/75 , A61L2/28 , C12M1/34 , C12M37/06 , C12N9/2471 , C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Y302/01023
Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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公开(公告)号：US10011844B2
公开(公告)日：2018-07-03
申请号：US15618157
申请日：2017-06-09
Applicant: American Sterilizer Company
Inventor： Phillip P. Franciskovich , Tricia A. Cregger , William A. Yirava , Peter A. Burke
IPC: C12Q1/22 , C12Q1/34 , C12Q1/68 , C12N15/75 , C12M1/12 , C12N9/38 , A61L2/28 , C12M1/34 , C12Q1/6897 , C12Q1/689
Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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公开(公告)号：US20170275633A1
公开(公告)日：2017-09-28
申请号：US15618158
申请日：2017-06-09
Applicant: American Sterilizer Company
Inventor： Phillip P. Franciskovich , Tricia A. Cregger , William A. Yirava , Peter A. Burke
CPC classification number: C12N15/75 , A61L2/28 , C12M1/34 , C12M37/06 , C12N9/2471 , C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Y302/01023
Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
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公开(公告)号：US20160312232A1
公开(公告)日：2016-10-27
申请号：US15204079
申请日：2016-07-07
Applicant: American Sterilizer Company
Inventor： Phillip P. Franciskovich , Tricia A. Cregger , William A. Yirava , Peter A. Burke
CPC classification number: C12N15/75 , A61L2/28 , C12M1/34 , C12M37/06 , C12N9/2471 , C12Q1/22 , C12Q1/34 , C12Q1/689 , C12Q1/6897 , C12Y302/01023
Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.
Abstract translation: 本发明涉及一种衍生自组合物的生物指示剂，其包含：宿主生物，其包含孢子形成细菌; 用于产生指示酶的报告基因; 调节基因; 以及用于将报道基因和调节基因插入宿主生物体的载体; 宿主生物体在其基因组中具有转座遗传元件，用于将插入序列插入调节基因; 所述插入序列包含转座酶，一对末端反向重复序列和用于表达转座酶的至少一个开放阅读框。 载体可能被宿主生物体吸收。 插入序列可插入调节基因。 宿主生物体可能经历孢子形成以形成生物指示剂。 公开了一种使用生物指示剂的方法和装置。
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