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公开(公告)号：US20210062226A1
公开(公告)日：2021-03-04
申请号：US17084023
申请日：2020-10-29
IPC分类号： C12N15/90 , C12N15/11 , C12N15/63 , C12Q1/686 , C12N9/22 , C12N15/113 , C12N15/10 , C12N15/70 , C12N15/74 , A61K38/46 , A01H6/46 , A01K67/027
摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.
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公开(公告)号：US20210062223A1
公开(公告)日：2021-03-04
申请号：US17079070
申请日：2020-10-23
IPC分类号： C12N15/90 , C12N15/11 , C12N15/63 , C12Q1/686 , C12N9/22 , C12N15/113 , C12N15/10 , C12N15/70 , C12N15/74 , A61K38/46 , A01H6/46 , A01K67/027
摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.
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公开(公告)号：US10577631B2
公开(公告)日：2020-03-03
申请号：US16383422
申请日：2019-04-12
IPC分类号： C12N9/22 , C12N15/113 , C12N15/11 , C12N15/10 , C12N15/63 , C12N15/70 , C12N15/74 , A61K38/46 , C12N15/90 , A01H6/46 , A01K67/027 , C12Q1/686 , A61K48/00
摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.
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公开(公告)号：US10526619B2
公开(公告)日：2020-01-07
申请号：US16380758
申请日：2019-04-10
IPC分类号： C12N9/22 , C12N15/113 , C12N15/11 , C12N15/10 , C12N15/63 , C12N15/70 , C12N15/74 , A61K38/46 , C12N15/90 , A01H6/46 , A01K67/027 , A61K48/00
摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.
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公开(公告)号：US10519467B2
公开(公告)日：2019-12-31
申请号：US15803424
申请日：2017-11-03
IPC分类号： C12N9/22 , C12N15/113 , C12N15/11 , C12N15/10 , C12N15/63 , C12N15/70 , C12N15/74 , A61K38/46 , C12N15/90 , A01H6/46 , A01K67/027 , A61K48/00
摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.
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公开(公告)号：US10308961B2
公开(公告)日：2019-06-04
申请号：US16033016
申请日：2018-07-11
IPC分类号： A61K38/46 , C12N9/22 , C12N15/10 , C12N15/11 , C12N15/113 , A01K67/027 , A01H6/46 , C12N15/70 , C12N15/74 , C12N15/90 , C12N15/63 , A61K48/00
摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.
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公开(公告)号：US10000772B2
公开(公告)日：2018-06-19
申请号：US14685502
申请日：2015-04-13
IPC分类号： C12N9/22 , C12N15/10 , C12N15/11 , C12N15/113 , C12N15/90 , C12N15/70 , C12N15/74 , C12N15/63
摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.
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18.发明授权公开(公告)号：US09952422B2
公开(公告)日：2018-04-24
申请号：US14996015
申请日：2016-01-14
申请人： University of Vienna
发明人： Alipasha Vaziri , Tobias Nöbauer
CPC分类号： G02B21/365 , G02B21/0072 , G02B21/0076 , G02B21/008 , G02B21/367 , G06T7/557 , G06T2207/10052 , H04N13/189 , H04N13/229 , H04N13/232
摘要： Methods and systems are provided for enhancing the imaging resolution of three dimensional imaging using light field microscopy. A first approach enhances the imaging resolution by modeling the scattering of light that occurs in an imaging target, and using the model to account for the effects of light scattering when de-convolving the light field information to retrieve the 3-dimensional (3D) volumetric information of the imaging target. A second approach enhances the imaging resolution by using a second imaging modality such as two-photon, multi-photon, or confocal excitation microscopy to determine the locations of individual neurons, and using the known neuron locations to enhance the extraction of time series signals from the light field microscopy data.
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公开(公告)号：US20160069794A1
公开(公告)日：2016-03-10
申请号：US14846934
申请日：2015-09-07
申请人： University of Vienna
发明人： Alipasha Vaziri , Robert Prevedel
IPC分类号： G01N21/25 , G01N33/483
CPC分类号： G01N33/4833 , A61B5/4064 , G01J3/4406 , G01N21/636 , G01N21/6458 , G01N2021/6419 , G01N2021/6421 , G01N2201/0635 , G01N2201/0697 , G01N2201/1045 , G02B21/02 , G02B21/16 , G02B2207/114
摘要： Devices and methods for recording dynamics of cellular and/or biochemical processes, including a device including one or more dispersive elements configured to receive a pulsed laser beam with a spectrum of different wavelengths and disperse the spectrum of the pulsed laser beam; and one or more first elements configured to receive the dispersed spectrum of the pulsed laser beam, and generate a multiphoton excitation area in a biological sample by re-overlapping in time and space the dispersed spectrum of the pulsed laser beam on an area in the biological sample, wherein the device is configured to record at high speed changes of cellular and biochemical processes of a population of cells of the biological sample based on generation of the multiphoton excitation area in the biological sample.
摘要翻译： 用于记录细胞和/或生物化学过程的动力学的装置和方法，包括包括一个或多个分散元件的装置，其被配置为接收具有不同波长的光谱的脉冲激光束并分散脉冲激光束的光谱; 以及被配置为接收脉冲激光束的分散光谱的一个或多个第一元件，并且通过在脉冲激光束的时间和空间上重新重叠生物样品中的多光子激发区域，使脉冲激光束的分散光谱在生物样品中的一个区域上 样品，其中所述装置被配置为基于所述生物样品中的多光子激发区域的产生，高速地记录所述生物样品的细胞群的细胞和生化过程的变化。
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公开(公告)号：US12123015B2
公开(公告)日：2024-10-22
申请号：US17481085
申请日：2021-09-21
IPC分类号： C12N15/90 , A01H6/46 , A01K67/027 , A61K38/46 , A61K48/00 , C12N9/22 , C12N15/10 , C12N15/11 , C12N15/113 , C12N15/63 , C12N15/70 , C12N15/74 , C12Q1/686 , H01L21/02 , H01L33/00
CPC分类号： C12N15/907 , A01H6/4684 , A01K67/027 , A61K38/465 , C12N9/22 , C12N15/102 , C12N15/111 , C12N15/113 , C12N15/63 , C12N15/70 , C12N15/746 , C12N15/90 , C12N15/902 , C12Q1/686 , H01L21/02312 , H01L21/02365 , H01L33/0075 , A61K48/00 , C12N2310/11 , C12N2310/13 , C12N2310/14 , C12N2310/20 , C12N2310/31 , C12N2310/32 , C12N2310/33 , C12N2310/3519 , C12N2310/531 , C12N2800/80 , C12Y301/04
摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.
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