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1918 条记录 (耗时 0.025 秒)

    Tissue-engineered three-dimensional model for tumor analysis
    93.
    发明授权
    Tissue-engineered three-dimensional model for tumor analysis 有权

    公开(公告)号：US10883083B2

    公开(公告)日：2021-01-05

    申请号：US16016101

    申请日：2018-06-22

    申请人： The Trustees of Columbia University in the City of New York

    发明人： Gordana Vunjak-Novakovic ,  Aranzazu Villasante

    IPC分类号： C12N5/077 ,  C12N5/09 ,  C12N5/071 ,  C12N5/0775 ,  C12N5/078

    摘要： A 3D decellularized bone scaffold seeded with cancer cells, such as prostate cancer cells or Ewing's sarcoma is provided. The three-dimensional includes Ewing's sarcoma (ES) tumor cells; and an engineered human bone scaffold. The engineered human bone scaffold further includes osteoblasts that secrete substance of the human bone, and osteoclasts that absorb bone tissue during growth and healing. The engineered human bone scaffold includes the tissue engineered three-dimensional model which recapitulates the osteolytic process. The engineered human bone scaffold is engineered by co-culturing of osteoblasts and osteoclasts. The osteoblast is produced by cell differentiation process from mesenchymal stem cells. The osteoclast is produced by cell differentiation from human monocytes, wherein the human monocytes are isolated from buffy coats. The scaffold can be used with cancer cell lines to identify therapeutic targets to slow, stop, and reverse tumor growth and progression as well as to predict the efficacy of potential therapeutics.

    Microdevice platform recapitulating hypoxic tissue microenvironments
    95.
    发明授权
    Microdevice platform recapitulating hypoxic tissue microenvironments 有权

    公开(公告)号：US10829730B2

    公开(公告)日：2020-11-10

    申请号：US15941374

    申请日：2018-03-30

    申请人： UNIVERSITY OF SOUTHERN CALIFORNIA

    发明人： Keyue Shen ,  Yuta Ando ,  Daniel Yen ,  Hoang Ta

    IPC分类号： C12M3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/06 ,  C12M3/04 ,  C12M1/12 ,  C12N5/09

    摘要： Hypoxia plays a central role in cancer progression and resistance to therapy. A microdevice platform is engineered to recapitulate the intratumor oxygen gradients that drive the heterogeneous hypoxic landscapes in solid tumors. The microdevice design features a “tumor section”-like culture by incorporating a cell layer between two diffusion barriers, where an oxygen gradient is established by cellular metabolism and physical constraints. The oxygen gradient is confirmed by numerical simulation and imaging-based oxygen sensor measurement. Spatially-resolved hypoxic signaling in cancer cells is also demonstrated through immunostaining, gene expression assay, and hypoxia-targeted drug treatment. The microdevice platform can accurately generate and control oxygen gradients, eliminates complex microfluidic handling, allows for incorporation of additional tumor components, and is compatible with high-content imaging and high-throughput applications. It is well suited for understanding hypoxia-mediated mechanisms in cancer disease and other biological tissues and processes, and discovery of new therapeutics.

    Methods of Primary Tissue Culture and Drug Screening Using Autologous Serum and Fluids
    96.
    发明申请
    Methods of Primary Tissue Culture and Drug Screening Using Autologous Serum and Fluids 审中-公开

    公开(公告)号：US20200347361A1

    公开(公告)日：2020-11-05

    申请号：US16921007

    申请日：2020-07-06

    申请人： Yao Tang

    发明人： Yao Tang

    IPC分类号： C12N5/09

    摘要： The present invention provides methods for culturing primary cells and tissues from a subject in the presence of the subject's own serum, ascites or pleural effusion fluid. Methods of treating cancer, and screening for the effectiveness or toxicity of drugs are also provided herein.

    METHOD FOR PRODUCING EXOSOMES AND EXOSOMES OBTAINED THEREBY
    97.
    发明申请
    METHOD FOR PRODUCING EXOSOMES AND EXOSOMES OBTAINED THEREBY 审中-公开

    公开(公告)号：US20200347360A1

    公开(公告)日：2020-11-05

    申请号：US16962880

    申请日：2019-01-17

    申请人： TOKYO INSTITUTE OF TECHNOLOGY

    发明人： Fumi KANO ,  Masayuki MURATA ,  Yuki SONODA

    IPC分类号： C12N5/09 ,  C12N15/113 ,  A61K9/50

    摘要： The object of the present invention is to provide a method for incorporating an arbitrary protein, lipid, carbohydrate, or nucleic acid into an exosome. The object can be solved by a method for preparing an exosome, comprising the steps of: (a) adding a biological toxin having a perforating activity to a medium containing cells and incubating the mixture, (b) adding ATP and incubating the mixture, and (c) adding a medium containing calcium ion and incubating the mixture.

    TUMOR ORGANOID MODEL
    99.
    发明申请
    TUMOR ORGANOID MODEL 审中-公开

    公开(公告)号：US20200308550A1

    公开(公告)日：2020-10-01

    申请号：US16646548

    申请日：2018-09-11

    申请人： IMBA - INSTITUT FÜR MOLEKULARE BIOTECHNOLOGIE GMBH

    发明人： Jürgen KNOBLICH ,  Shan BIAN

    IPC分类号： C12N5/09 ,  C12N5/079 ,  C12N5/071 ,  G01N33/50

    摘要： A method of generating an artificial 3D tissue culture of a cancer grown in non-cancerous tissue, includes the steps of providing an aggregate of pluripotent stem cells or progenitor cells, culturing and expanding the cells in a 3D biocompatible matrix, wherein the cells are allowed to differentiate to develop the aggregate into a tissue culture of a desired size; wherein at least a portion of the cells are subjected to cancerogenesis by expressing a oncogene and/or by suppressing a tumor suppressor gene during any of the steps or in the tissue culture, and further including the step of allowing the cells with an expressed oncogene or suppressed tumor suppressor to develop into cancerous cells; drug screening methods; oncolytic virus screening methods; a 3D tissue culture; and a kit for performing the inventive methods.

    METHODS AND SYSTEMS FOR CELL-BASED NON-INVASIVE PRENATAL TESTING
    100.
    发明申请
    METHODS AND SYSTEMS FOR CELL-BASED NON-INVASIVE PRENATAL TESTING 审中-公开

    公开(公告)号：US20200290045A1

    公开(公告)日：2020-09-17

    申请号：US15930407

    申请日：2020-05-12

    申请人： Lian Liu

    发明人： Lian Liu

    IPC分类号： B01L3/00 ,  A61B5/15 ,  C12N5/09 ,  G01N33/50

    摘要： Methods and systems are provided for isolating fetal cells from a maternal blood supply in order to perform non-invasive prenatal testing. In one example, a system for non-invasive prenatal testing includes a substrate coated with a cell-capturing surface, the cell-capturing surface including an array of pillar-like structures, each pillar-like structure including a plurality of intersecting arms.