公开(公告)号：US20250044212A1

公开(公告)日：2025-02-06

申请号：US18800710

申请日：2024-08-12

Applicant: ThinkCyte K.K.

Inventor： Toru Imai ,  Keiji Nakagawa

IPC: G01N15/1409 ,  G01N15/14 ,  G01N15/1429

Abstract: A flow cytometer includes a microfluidic device, a light source, a photodetector, an information generation device, a calculation device, and a flow path position control device. In the microfluidic device, a first position detection line is arranged on a flow path, a second position detection line is arranged with a portion overlapping the first position detection line in a width direction, and a position detection distance, which is a distance between the first position detection line and the second position detection line in a length direction of the flow path, changes with a position in the width direction. The calculation device includes a time difference calculation unit configured to calculate a time difference between the time when the photodetector has detected a peak intensity of an optical signal at any one detection position on the first position detection line and the time when the photodetector has detected the peak intensity of the optical signal at any one detection position on the second position detection line and a position calculation unit configured to calculate the position of the observation object in the width direction on the basis of the time difference and a corresponding relationship between the time difference and the position in the width direction.

公开(公告)号：US20250044211A1

公开(公告)日：2025-02-06

申请号：US18800748

申请日：2024-08-12

Applicant: ThinkCyte K.K.

Inventor： Toru Imai ,  Hiroaki Adachi

IPC: G01N15/1404 ,  G01N15/14 ,  G01N15/1434

Abstract: A flow cytometer includes a microfluidic device, a light source, a photodetector that detects, in time series, the intensity of signal light emitted from an observation object when the observation object flowing through a flow path is irradiated with illumination light, an information generation device that generates optical information indicating a structure of the observation object based on the intensity of the signal light, an arithmetic device, and a flow path position control device, wherein the arithmetic device includes a signal intensity acquiring unit that acquires electronic data of temporal changes in the intensity of the signal light detected based on a detection position predetermined in the flow path to detect the depth position in the flow path where the observation object passes through the flow path, a scan unit that performs a scan process to move the flow path in the depth direction and acquire the electronic data at different depth positions, a position calculation unit that calculates the depth position based on the electronic data, and an output unit that outputs position information indicating the depth position calculated by the position calculation unit.

公开(公告)号：US20240410810A1

公开(公告)日：2024-12-12

申请号：US18700652

申请日：2021-10-15

Applicant: ThinkCyte K.K.

Inventor： Toru Imai ,  Keiji Nakagawa

IPC: G01N15/1409 ,  G01N15/14 ,  G01N15/1429

Abstract: A flow cytometer includes a microfluidic device, a light source, a photodetector, an information generation device, a calculation device, and a flow path position control device. In the microfluidic device, a first position detection line is arranged on a flow path, a second position detection line is arranged with a portion overlapping the first position detection line in a width direction, and a position detection distance, which is a distance between the first position detection line and the second position detection line in a length direction of the flow path, changes with a position in the width direction. The calculation device includes a time difference calculation unit configured to calculate a time difference between the time when the photodetector has detected a peak intensity of an optical signal at any one detection position on the first position detection line and the time when the photodetector has detected the peak intensity of the optical signal at any one detection position on the second position detection line and a position calculation unit configured to calculate the position of the observation object in the width direction on the basis of the time difference and a corresponding relationship between the time difference and the position in the width direction.

公开(公告)号：US20230266229A1

公开(公告)日：2023-08-24

申请号：US18118670

申请日：2023-03-07

Applicant: ThinkCyte K.K.

Inventor： Toru Imai ,  Hiroaki Adachi ,  Keisuke Toda

IPC: G01N15/14

CPC classification number: G01N15/1459 ,  G01N15/1484 ,  G01N15/1436 ,  G01N2015/1006

Abstract: A flow cytometer includes: a light source; a microfluidic device; a photodetector; an information generation device which generates optical information indicating a morphology of an observation target on the basis of optical signal intensity; and a spatial light modulation unit which is installed on an optical path between the light source and the photodetector and structures any one of illumination light irradiated from the light source toward the flow path and signal light from the observation target. In the flow path provided with the microfluidic device, a plurality of optical signal detection positions are arranged linearly at equal intervals in a predetermined direction of the flow path by structuring the illumination light or the signal light and a plurality of trigger signal detection positions for detecting a trigger signal by which the information generation device starts the generation of the optical information are arranged to be separated by the same predetermined distance in a length direction of the flow path while respectively corresponding to the plurality of optical signal detection positions.

公开(公告)号：US20250060301A1

公开(公告)日：2025-02-20

申请号：US18915034

申请日：2024-10-14

Applicant: ThinkCyte K.K.

Inventor： Toru Imai ,  Keisuke Toda

IPC: G01N15/14 ,  G01N15/10 ,  G01N15/1433 ,  G01N15/1434

Abstract: A flow cytometer includes: a flow channel allowing a measurement object not labeled with a fluorescent dye to flow along with a fluid; a light source emitting illumination light; an illumination optical system irradiating the measurement object flowing in the flow channel with the illumination light emitted from the light source as a spotlight which is illumination light condensed in at least one of a width direction and a depth direction of the flow channel at an irradiation position in the flow channel; a light detector detecting fluorescence emitted from a specific molecule of the measurement object in response to irradiation with the spotlight via the illumination optical system; a detection optical system allowing the fluorescence to propagate to the light detector; and a discrimination unit configured to discriminate whether the measurement object is a target measurement object based on information of the fluorescence detected by the light detector.