公开(公告)号：US20250044212A1

公开(公告)日：2025-02-06

申请号：US18800710

申请日：2024-08-12

Applicant: ThinkCyte K.K.

Inventor： Toru Imai ,  Keiji Nakagawa

IPC: G01N15/1409 ,  G01N15/14 ,  G01N15/1429

Abstract: A flow cytometer includes a microfluidic device, a light source, a photodetector, an information generation device, a calculation device, and a flow path position control device. In the microfluidic device, a first position detection line is arranged on a flow path, a second position detection line is arranged with a portion overlapping the first position detection line in a width direction, and a position detection distance, which is a distance between the first position detection line and the second position detection line in a length direction of the flow path, changes with a position in the width direction. The calculation device includes a time difference calculation unit configured to calculate a time difference between the time when the photodetector has detected a peak intensity of an optical signal at any one detection position on the first position detection line and the time when the photodetector has detected the peak intensity of the optical signal at any one detection position on the second position detection line and a position calculation unit configured to calculate the position of the observation object in the width direction on the basis of the time difference and a corresponding relationship between the time difference and the position in the width direction.

公开(公告)号：US12235202B2

公开(公告)日：2025-02-25

申请号：US17847478

申请日：2022-06-23

Applicant: ThinkCyte K.K.

Inventor： Keiji Nakagawa

IPC: G01N15/14 ,  G01N15/10 ,  G01N15/149

Abstract: A method of evaluating performance of a flow cytometer configured to use a combination of two or more types of calibration particles having different morphologies from each other, includes a first classification step of classifying the calibration particles from each other based on a first optical characteristic by the flow cytometer which is an evaluation target, a second classification step of classifying the calibration particles from each other based on a second optical characteristic which is classifiable at a spatial resolution lower than a spatial resolution at which the first optical characteristic is classified, and the evaluation step of evaluating one or both of particle classification performance and a resolution of the flow cytometer based on a first classification result assessed in the first classification step and a second classification result assessed in the second classification step.

公开(公告)号：US20240410810A1

公开(公告)日：2024-12-12

申请号：US18700652

申请日：2021-10-15

Applicant: ThinkCyte K.K.

Inventor： Toru Imai ,  Keiji Nakagawa

IPC: G01N15/1409 ,  G01N15/14 ,  G01N15/1429

Abstract: A flow cytometer includes a microfluidic device, a light source, a photodetector, an information generation device, a calculation device, and a flow path position control device. In the microfluidic device, a first position detection line is arranged on a flow path, a second position detection line is arranged with a portion overlapping the first position detection line in a width direction, and a position detection distance, which is a distance between the first position detection line and the second position detection line in a length direction of the flow path, changes with a position in the width direction. The calculation device includes a time difference calculation unit configured to calculate a time difference between the time when the photodetector has detected a peak intensity of an optical signal at any one detection position on the first position detection line and the time when the photodetector has detected the peak intensity of the optical signal at any one detection position on the second position detection line and a position calculation unit configured to calculate the position of the observation object in the width direction on the basis of the time difference and a corresponding relationship between the time difference and the position in the width direction.