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公开(公告)号：US08969004B2
公开(公告)日：2015-03-03
申请号：US13895207
申请日：2013-05-15
Applicant: Life Technologies Corporation
Inventor： Matthew Beaudet , William Gregory Cox , Stephen Yue
IPC: C12Q1/68 , C07D209/04 , C07H21/02 , C07H19/04
CPC classification number: G01N21/64 , C07D417/06 , C09B23/02 , C09B23/04 , C12Q1/6816 , C12Q1/6825 , G01N1/30 , G01N2001/302 , Y10T436/143333 , C12Q2563/173 , C12Q2565/125
Abstract: The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid.
Abstract translation: 本发明提供了使用作为噻唑橙衍生物的不对称花青染料确定固定化核酸的存在的方法，染色溶液和选择性荧光化合物，其特征在于基本上是非遗传毒性的。 所述方法包括在固体或半固体支持物上固定核酸，单链或双链DNA，RNA或其组合，使固定的核酸与不对称的花青染料化合物接触，然后用合适的波长照射固定的核酸，由此 确定核酸的存在。 花青染料化合物通常存在于包含染料化合物和三乙酸盐或三硼酸盐缓冲液的水性染色溶液中，其中溶液促进染料化合物和固定化核酸的接触。
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公开(公告)号：US11635383B2
公开(公告)日：2023-04-25
申请号：US17211154
申请日：2021-03-24
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Matthew Beaudet , Jill Hendrickson , David Hagen , Rich Meyer
Abstract: Devices and methods for measuring the quantity of multiple analytes in a sample can include a device designed such that each of the analyte sensing elements is configured to measure the quantity of a predetermined analyte and machine executable instructions configured to select the proper analyte sensing element corresponding to the analyte to be measured.
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Patent Agency Ranking
公开(公告)号：US10962480B2
公开(公告)日：2021-03-30
申请号：US16735856
申请日：2020-01-07
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Matthew Beaudet , Jill Hendrickson , David Hagen , Rich Meyer
Abstract: Devices and methods for measuring the quantity of multiple analytes in a sample can include a device designed such that each of the analyte sensing elements is configured to measure the quantity of a predetermined analyte and machine executable instructions configured to select the proper analyte sensing element corresponding to the analyte to be measured.
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公开(公告)号：US20160003743A1
公开(公告)日：2016-01-07
申请号：US14851046
申请日：2015-09-11
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Matthew Beaudet , Jill HENDRICKSON , David HAGEN , Rich MEYER
IPC: G01N21/64
CPC classification number: G01N21/6486 , B01L3/5082 , B01L7/52 , B01L2200/0647 , B01L2200/143 , G01N21/6428 , G01N21/6452 , G01N2015/1486 , G01N2021/6441 , G01N2035/00782 , Y10T436/143333
Abstract: The present invention relates to devices and methods for measuring the quantity of multiple analytes in a sample. The device is designed such that each of the analyte sensing elements is configured to measure the quantity of a predetermined analyte and where the machine executable instructions are configured to select the proper analyte sensing element corresponding to the analyte to be measured.
Abstract translation: 本发明涉及用于测量样品中多种分析物的量的装置和方法。 该设备被设计成使得每个分析物感测元件被配置为测量预定分析物的量，并且其中机器可执行指令被配置为选择与待测量的分析物相对应的适当的分析物感测元件。
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公开(公告)号：US20140011191A1
公开(公告)日：2014-01-09
申请号：US14017965
申请日：2013-09-04
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Matthew Beaudet , Jill Hendrickson , David Hagen , Rich Meyer
IPC: G01N21/64
CPC classification number: G01N21/6486 , B01L3/5082 , B01L7/52 , B01L2200/0647 , B01L2200/143 , G01N21/6428 , G01N21/6452 , G01N2015/1486 , G01N2021/6441 , G01N2035/00782 , Y10T436/143333
Abstract: The present invention relates to devices and methods for measuring the quantity of multiple analytes in a sample. The device is designed such that each of the analyte sensing elements is configured to measure the quantity of a predetermined analyte and where the machine executable instructions are configured to select the proper analyte sensing element corresponding to the analyte to be measured.
Abstract translation: 本发明涉及用于测量样品中多种分析物的量的装置和方法。 该设备被设计成使得每个分析物感测元件被配置为测量预定分析物的量，并且其中机器可执行指令被配置为选择与待测量的分析物相对应的适当的分析物感测元件。
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公开(公告)号：US10533946B2
公开(公告)日：2020-01-14
申请号：US15922477
申请日：2018-03-15
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Matthew Beaudet , Jill Hendrickson , David Hagen , Rich Meyer
Abstract: The present invention relates to devices and methods for measuring the quantity of multiple analytes in a sample. The device is designed such that each of the analyte sensing elements is configured to measure the quantity of a predetermined analyte and where the machine executable instructions are configured to select the proper analyte sensing element corresponding to the analyte to be measured.
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公开(公告)号：US09964490B2
公开(公告)日：2018-05-08
申请号：US14851046
申请日：2015-09-11
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Matthew Beaudet , Jill Hendrickson , David Hagen , Rich Meyer
CPC classification number: G01N21/6486 , B01L3/5082 , B01L7/52 , B01L2200/0647 , B01L2200/143 , G01N21/6428 , G01N21/6452 , G01N2015/1486 , G01N2021/6441 , G01N2035/00782 , Y10T436/143333
Abstract: The present invention relates to devices and methods for measuring the quantity of multiple analytes in a sample. The device is designed such that each of the analyte sensing elements is configured to measure the quantity of a predetermined analyte and where the machine executable instructions are configured to select the proper analyte sensing element corresponding to the analyte to be measured.
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