公开(公告)号：US20130140437A1

公开(公告)日：2013-06-06

申请号：US13691382

申请日：2012-11-30

Applicant: Leica Microsystems CMS GmbH

Inventor： Bernd WIDZGOWSKI

IPC: G01J1/44

CPC classification number: G01J1/44 ,  G01N21/6408 ,  G01N21/6458 ,  G02B21/0076 ,  G02B21/0084

Abstract: A method and an apparatus for examining a sample. The apparatus has a light source for generating excitation light in pulses which occur in succession at an excitation pulse frequency, for illuminating a sample region with the excitation pulse, and having a detector for detecting the detection light emanating from the sample region. The apparatus is characterized in that, for each detected photon of the detection light, the detector generates an electrical pulse and thereby a sequence of electrical pulses, and an analog-digital converter is provided that generates a digital data sequence by sampling the sequence of electrical pulses at a sampling rate.

Abstract translation: 一种用于检查样品的方法和装置。 该装置具有用于产生以激励脉冲频率连续发生的脉冲的激发光的光源，用于用激发脉冲照射样本区域，并具有用于检测从样本区域发出的检测光的检测器。 该装置的特征在于，对于检测光的每个检测到的光子，检测器产生电脉冲，从而提供一系列电脉冲，并且提供模数转换器，其通过采样电气序列生成数字数据序列 脉冲采样率。

公开(公告)号：US20240280798A1

公开(公告)日：2024-08-22

申请号：US18443399

申请日：2024-02-16

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Falk SCHLAUDRAFF ,  Sam STEBLAU ,  Eugen NORDHEIMER ,  Bernd WIDZGOWSKI

IPC: G02B21/36 ,  H04N23/76 ,  H04N23/81

CPC classification number: G02B21/365 ,  H04N23/76 ,  H04N23/81

Abstract: An apparatus for data processing for a digital imaging device is provided. The digital imaging device is configured to generate a digital image of a recording region by reading out, raster-element-by-raster-element, a multidimensional complete raster. The complete raster includes a plurality of raster elements. The apparatus is part of a control unit of the imaging device or is configured to be controllable by the control unit of the imaging device. The apparatus is configured to process raw image data from at least one sub-region of the complete raster that has already been read out during the reading out, generate processed image data in at least one processing step as a function of the raw image data, and make the processed image data available for display for access from outside the apparatus.

公开(公告)号：US20130119276A1

公开(公告)日：2013-05-16

申请号：US13676408

申请日：2012-11-14

Applicant: Leica Microsystems CMS GmbH

Inventor： Bernd WIDZGOWSKI

IPC: G01N21/64

CPC classification number: G01N21/6408 ,  G01N21/6458 ,  G01N2201/0697 ,  G01N2201/12

Abstract: The present invention relates to a method for measuring the lifetime of an excited state in a sample, in particular a fluorescence lifetime, and to an apparatus for carrying out such a method. First, an excitation pulse is generated and a sample region is illuminated with the excitation pulse. Then, a first digital data sequence is generated which is representative of the power-time profile of the excitation pulse, and a first switching instant is determined from the first digital data sequence. Moreover, the detection light emanating from the sample region is detected by a detector, and a second digital data sequence is generated which is representative of the power-time profile of the detection light, and a second switching instant is determined from the second digital data sequence. Finally, the time difference between the first and second switching instants is calculated.

Abstract translation: 本发明涉及一种用于测量样品中的激发态的寿命的方法，特别是涉及荧光寿命的方法以及用于实施这种方法的装置。 首先，产生激发脉冲，用激发脉冲照射采样区域。 然后，产生表示激励脉冲的功率 - 时间曲线的第一数字数据序列，并且从第一数字数据序列确定第一切换时刻。 此外，由检测器检测从采样区域发出的检测光，并且生成表示检测光的功率 - 时间曲线的第二数字数据序列，并且从第二数字数据确定第二切换时刻 序列。 最后，计算第一和第二切换时刻之间的时间差。

公开(公告)号：US20190086257A1

公开(公告)日：2019-03-21

申请号：US16081427

申请日：2017-03-01

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Vishnu Vardhan KRISHNAMACHARI ,  Patric MRAWEK ,  Bernd WIDZGOWSKI

IPC: G01J1/02 ,  G01J1/46 ,  G01J3/44

Abstract: A light/voltage converter circuit for converting intensity fluctuations of light into an alternating voltage measurement signal includes a photodiode configured to detect the light and a transformer comprising a primary coil and a secondary coil. The primary coil is connected in a series circuit to the photodiode, and the alternating voltage measurement signal is applied to the secondary coil. An electrical network is configured to block direct current and conduct alternating current. The electrical network is connected in parallel with the series circuit consisting of the primary coil of the transformer and the photodiode.

公开(公告)号：US20190339500A1

公开(公告)日：2019-11-07

申请号：US16305902

申请日：2017-05-31

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Bernd WIDZGOWSKI ,  Vishnu Vardhan KRISHNAMACHARI ,  Lars FRIEDRICH ,  Manuel KREMER ,  Arnold GISKE

IPC: G02B21/00

Abstract: An optical arrangement has an optical beam path for illuminating a sample space with a sequence of laser light pulses generated in a laser cycle, the optical arrangement. At least one laser light source is configured to generate the sequence of laser light pulses along the optical beam path. A wavelength-selective pulse picker is situated in the optical beam path and has, in a predefined illumination clock timing synchronizable with the laser light pulses, an open state in which the pulse picker is light-transparent to at least one laser light pulse towards the sample space. The open state has at least two different transmission states which differ with regard to their respective transmission spectrums, and wherein the two transmission states are switchable on and/or off independently of one another.

公开(公告)号：US20190339201A1

公开(公告)日：2019-11-07

申请号：US16304168

申请日：2017-05-24

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Volker SEYFRIED ,  Bernd WIDZGOWSKI ,  Frank HECHT

IPC: G01N21/64

Abstract: A fluorescence lifetime imaging microscopy method with time-correlated single photon counting includes periodically exciting a sample to emit fluorescence photons, with a measurement interval being defined between each two successive excitation light pulses. A value characterizing fluorescence decay behavior is determined based on detection times of detected fluorescence photons, and imaging is performed based one the value. An analog detector signal is sampled within a plurality of sampling intervals within a respective one of the measurement intervals and is converted into a sequence of discrete signal values associated with the sampling intervals. It is determined based thereon whether more than a predefined number of fluorescence photons has been detected within the respective measurement interval. If more than the predefined number of fluorescence photons has been detected, the respective measurement interval is discarded in the step of determining the value characterizing the fluorescence decay behavior.