公开(公告)号：US20240018520A1

公开(公告)日：2024-01-18

申请号：US18340838

申请日：2023-06-24

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup LEE ,  Dongsoo YANG ,  Jae Sung CHO

IPC: C12N15/113 ,  C12N15/63

CPC classification number: C12N15/113 ,  C12N2310/10 ,  C12N15/63

Abstract: Synthetic sRNA for inhibiting prokaryote gene expression is described, which includes (i) an Hfq binding site and (ii) a region that forms a complementary bond with a target gene mRNA. Vectors encoding the synthetic sRNA are described, as well as recombinant prokaryotes transformed with such vectors, methods of inhibiting prokaryote gene expression, and methods of gene screening and strain improvement. The synthetic sRNA is able to control single and multiple target genes at a time, and is particularly useful for inhibiting a gene expression of Gram-positive bacteria, e.g., in a recombinant Corynebacterium for mass production of high value products that do not require fossil fuels with associated environmental problems.

公开(公告)号：US20210214753A1

公开(公告)日：2021-07-15

申请号：US17055971

申请日：2019-04-24

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY ,  HANWHA SOLUTIONS CORPORATION

Inventor： Sang Yup LEE ,  Jae Sung CHO ,  Je Woong KIM ,  Cindy Pricilia Surya PRABOWO ,  Taehee HAN ,  Yoo Sung KO

IPC: C12P7/18 ,  C07K14/34 ,  C12N15/77 ,  C12N15/67

Abstract: The present invention relates to recombinant Corynebacterium having 1,3-PDO productivity and reduced 3-HP productivity, and a method for producing 1,3-PDO by using same. When a Corynebacterium glutamicum variant according to the present invention is used, the productivity of 3-HP, which is a by-product, is inhibited by using low-cost glycerol as a carbon source, and thus 1,3-PDO can be produced with high efficiency.

公开(公告)号：US20210062232A1

公开(公告)日：2021-03-04

申请号：US16962213

申请日：2019-01-15

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY ,  HANHWA SOLUTIONS CORPORATION

Inventor： Sang Yup LEE ,  Jae Sung CHO ,  Je Woong KIM ,  Yoo Sung KO ,  Cindy Pricilia Surya PRABOWO ,  Taehee HAN ,  Euiduk KIM ,  Jae Won CHOI ,  Changhee CHO ,  Jun Kyu LEE

IPC: C12P7/18 ,  C12N15/77 ,  C12N9/12 ,  C12N9/04 ,  C12N9/88

Abstract: The present disclosure relates to a mutant microorganism in which a glycerol catabolic pathway and a 1,3-PDO biosynthetic pathway are introduced into a microorganism incapable of using glycerol as a carbon source, and a method of producing 1,3-PDO using the same. According to the present disclosure, it is possible to produce 1,3-PDO while growing a mutant microorganism having 1,3-PDO production ability by using the inexpensive raw material glycerol as a single carbon source. Thus, the present disclosure is useful for the economical production of 1,3-PDO.

公开(公告)号：US20210054375A1

公开(公告)日：2021-02-25

申请号：US16960064

申请日：2019-03-08

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup LEE ,  Dongsoo YANG ,  Jae Sung CHO

IPC: C12N15/113 ,  C12N15/77 ,  C12Q1/689

Abstract: The present disclosure relate to a composition for inhibiting a prokaryotic expression and a use thereof and, more specifically, to a composition for inhibiting an expression of Gram-positive bacteria, which includes an sRNA comprising an sRNA-derived Hfq binding site from prokaryotes and (ii) a region that forms a complementary bond with a target gene mRNA and an Hfq from prokaryotes, a method of producing same, and a use thereof. A synthetic sRNA according to the present disclosure and a composition comprising the sRNA for inhibiting a gene expression having an advantage of being able to control single and multiple target genes at a time, can effectively reduce the expression of the target gene without the conventional gene deletion process via the synthetic sRNA that controls a gene expression so as to be useful for the production of a recombinant microorganism, and are particularly useful for inhibiting a gene expression of Gram-positive bacteria. A recombinant Corynebacterium produced by the present disclosure is a recombinant microorganism capable of mass production of high value products in an ecofriendly and reproducible manner on a bio-basis by controlling microbial metabolism flow through the synthetic sRNA. The recombinant microorganism, which is a bio-based production system developed through the sRNA is useful because of being able to replace existing fossil fuels while resolving environmental problems due to the ever-increasing use of crude oil.

公开(公告)号：US20200270587A1

公开(公告)日：2020-08-27

申请号：US16792229

申请日：2020-02-15

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup LEE ,  Zi Wei LUO ,  Jae Sung CHO

IPC: C12N9/10 ,  C12P13/00 ,  C12N1/20 ,  C12N15/77 ,  C12N15/70

Abstract: Disclosed are a recombinant microorganism capable of producing methyl anthranilate, wherein the recombinant microorganism is obtained by introducing an aamt1 gene into a microorganism having the capacity to produce anthranilic acid (ANT), and a method of producing methyl anthranilate using the same. The recombinant microorganism is capable of producing methyl anthranilate using only a purely biological process from renewable carbon-circulating biomass without chemical catalytic reaction, thus having an advantage of enabling mass production of high value-added methyl anthranilate in a very economical manner based on an environmentally friendly and simple production process.