公开(公告)号：US20220155297A1

公开(公告)日：2022-05-19

申请号：US17423133

申请日：2019-11-06

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Yuji KIMURA ,  Sayaka KAZAMI ,  Yu HASHIMOTO ,  Hiroyasu ITOH

IPC: G01N33/566 ,  G01N33/533 ,  G01N21/78 ,  C12M1/40

Abstract: A method for selecting cells highly responsive to a target substance according to the present invention comprises step of: (1a) bringing a sample containing a target substance into contact with a plurality of cells, wherein the cells are cells having a receptor for the target substance and a fluorescent indicator, and the fluorescent indicator emits fluorescence as a result of binding between the target substance and the receptor; (1b) calculating a fluorescence intensity increase rate for each of the cells; and (1c) selecting arbitrary cells exhibiting a fluorescence intensity increase rate within the top 50% among the plurality of cells. According to the present invention, it is possible to detect the target substance in the sample with high sensitivity.

公开(公告)号：US20230034756A1

公开(公告)日：2023-02-02

申请号：US17791280

申请日：2021-01-14

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Kazuhiko FUJIWARA ,  Sayaka KAZAMI ,  Yoshihiro MARUYAMA

IPC: G01N33/569 ,  G01N21/65

Abstract: A cell analysis method includes a mixing step of mixing a cell as an analyte, a metal ion solution, and a reducing agent to prepare a mixture solution, a metal microstructure generation step of reducing metal ions in the mixture solution by reducing action of the reducing agent to generate a metal microstructure on a support, and attaching the cell or a cell-derived substance to the metal microstructure, a drying step of, after the metal microstructure generation step, drying the support, a measurement step of, after the drying step, irradiating the metal microstructure on the support with excitation light, and measuring a spectrum of Raman scattered light generated by the excitation light irradiation, and an analysis step of analyzing the cell based on the spectrum of the Raman scattered light.

公开(公告)号：US20210302368A1

公开(公告)日：2021-09-30

申请号：US17259016

申请日：2019-06-19

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Yu HASHIMOTO ,  Sayaka KAZAMI

IPC: G01N27/447

Abstract: The support body includes a concentrating portion, which concentrates the object, between the first end portion and the second end portion. When an area of a cut section of the flow path when cut at a predetermined position in the first direction along a plane orthogonal to the first direction is a flow path cross-sectional area, a flow path cross-sectional area of the concentrating portion is smaller than a flow path cross-sectional area in a first region closer to a first end portion side than the concentrating portion, and a flow path cross-sectional area of a second region closer to the second end portion side than the concentrating portion is larger than the flow path cross-sectional area of the concentrating portion.