公开(公告)号：US20230034756A1

公开(公告)日：2023-02-02

申请号：US17791280

申请日：2021-01-14

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Kazuhiko FUJIWARA ,  Sayaka KAZAMI ,  Yoshihiro MARUYAMA

IPC: G01N33/569 ,  G01N21/65

Abstract: A cell analysis method includes a mixing step of mixing a cell as an analyte, a metal ion solution, and a reducing agent to prepare a mixture solution, a metal microstructure generation step of reducing metal ions in the mixture solution by reducing action of the reducing agent to generate a metal microstructure on a support, and attaching the cell or a cell-derived substance to the metal microstructure, a drying step of, after the metal microstructure generation step, drying the support, a measurement step of, after the drying step, irradiating the metal microstructure on the support with excitation light, and measuring a spectrum of Raman scattered light generated by the excitation light irradiation, and an analysis step of analyzing the cell based on the spectrum of the Raman scattered light.

公开(公告)号：US20210181116A1

公开(公告)日：2021-06-17

申请号：US17263175

申请日：2019-07-16

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Kazuhiko FUJIWARA ,  Yoshihiro MARUYAMA

IPC: G01N21/65 ,  G01N21/77

Abstract: An analyte analysis method includes, in one example, a mixing step of mixing an analyte, a metal ion solution, and a reducing agent to prepare a mixture solution, a metal microstructure generation step of reducing metal ions in the mixture solution by reducing action of the reducing agent in the mixture solution to generate a metal microstructure on a support, and attaching the analyte or a substance derived from the analyte to the metal microstructure, a measurement step of irradiating the metal microstructure on the support with excitation light, and measuring a spectrum of Raman scattered light generated by the excitation light irradiation, and an analysis step of analyzing the analyte based on the spectrum of the Raman scattered light.

公开(公告)号：US20220172045A1

公开(公告)日：2022-06-02

申请号：US17437871

申请日：2020-02-25

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Masashi FUKUHARA ,  Kazuhiko FUJIWARA ,  Yoshihiro MARUYAMA

IPC: G06N3/08 ,  G06N3/063 ,  G06K9/62

Abstract: A convolutional neural network decision basis extraction apparatus includes a contribution rate calculation unit and a basis extraction unit. The contribution rate calculation unit obtains a contribution rate of a weight of a fully connected layer to an output label of an output layer. The basis extraction unit extracts a decision basis of a CNN based on a feature map input to the fully connected layer, the weight of the fully connected layer, and the above contribution rate.

公开(公告)号：US20210190679A1

公开(公告)日：2021-06-24

申请号：US16755720

申请日：2018-09-26

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Masashi FUKUHARA ,  Kazuhiko FUJIWARA ,  Yoshihiro MARUYAMA

IPC: G01N21/27 ,  G06K9/62 ,  G01N21/65 ,  G06N3/04 ,  G06N3/08

Abstract: A spectrum analysis apparatus is an apparatus for analyzing an analysis object on the basis of a spectrum of light generated in the analysis object containing any one or two or more of a plurality of reference objects, and includes an array conversion unit, a processing unit, a learning unit, and an analysis unit. The array conversion unit generates two-dimensional array data on the basis of a spectrum of light generated in the reference object or the analysis object. The processing unit includes a deep neural network. The analysis unit causes the array conversion unit to generate the two-dimensional array data on the basis of the spectrum of light generated in the analysis object, inputs the two-dimensional array data to the deep neural network, and analyzes the analysis object on the basis of data output from the deep neural network.

公开(公告)号：US20180328852A1

公开(公告)日：2018-11-15

申请号：US15776517

申请日：2016-11-15

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Kazuhiko FUJIWARA ,  Yoshihiro MARUYAMA

IPC: G01N21/78

CPC classification number: G01N21/78 ,  G01N21/82 ,  G01N31/22

Abstract: A concentration measurement method is a method for measuring a concentration of an analyte in a measurement solution containing the analyte having reducing action, and includes a mixing step of preparing a mixture solution by mixing a metal ion solution, a complexing agent, and a pH adjusting agent to prepare an intermediate mixture solution, and mixing the intermediate mixture solution and the measurement solution, a metal microstructure generation step of generating a metal microstructure on a support by reducing metal ions in the mixture solution by the reducing action of the analyte in the mixture solution, a measurement step of measuring an optical response of the metal microstructure on the support, and an analysis step of determining a concentration of the analyte in the measurement solution on the basis of a measurement result of the optical response.