公开(公告)号：US09689840B2

公开(公告)日：2017-06-27

申请号：US15015415

申请日：2016-02-04

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Thomas R. Berkelman ,  Lisandra Martin ,  Rhiannon Jones ,  John Walker, II

IPC: C07D255/02 ,  G01N27/447 ,  C08F220/60 ,  G01N33/543 ,  C07F3/06 ,  C07F13/00 ,  C08F220/56 ,  B01D15/38

CPC classification number: G01N27/44747 ,  B01D15/3823 ,  C07D255/02 ,  C07F3/06 ,  C07F13/005 ,  C08F220/56 ,  C08F220/60 ,  G01N33/54306 ,  Y10T436/143333 ,  Y10T436/163333

Abstract: Conjugates of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes with a variety of conjugating members are used in the formation of dinuclear metal complexes which bind to phosphate esters. By virtue of their conjugated forms, the complexes are incorporated into chromatographic media, affinity binding reagents, and dyes, which make the complexes useful in a wide range of assays, separations, and purifications. In addition, dinuclear metal complexes of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes that are not so conjugated are used in the detection of phosphate esters of biological species by either MALDI-TOF mass spectrometry or by dye displacement.

公开(公告)号：US10119934B2

公开(公告)日：2018-11-06

申请号：US15272257

申请日：2016-09-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Craig Rowell ,  Cory Panattoni ,  Thomas R. Berkelman ,  Sean Cater ,  Shane Petersen ,  Lee Olech ,  Xuemei Yang

IPC: G01N27/447 ,  B01D57/02 ,  C08F220/56

Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

公开(公告)号：US20160223491A1

公开(公告)日：2016-08-04

申请号：US15015415

申请日：2016-02-04

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Thomas R. Berkelman ,  Lisandra Martin ,  Rhiannon Jones ,  John Walker, II

IPC: G01N27/447 ,  G01N33/543 ,  C07F3/06 ,  C07F13/00 ,  C08F220/56 ,  C07D255/02

CPC classification number: G01N27/44747 ,  B01D15/3823 ,  C07D255/02 ,  C07F3/06 ,  C07F13/005 ,  C08F220/56 ,  C08F220/60 ,  G01N33/54306 ,  Y10T436/143333 ,  Y10T436/163333

Abstract: Conjugates of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes with a variety of conjugating members are used in the formation of dinuclear metal complexes which bind to phosphate esters. By virtue of their conjugated forms, the complexes are incorporated into chromatographic media, affinity binding reagents, and dyes, which make the complexes useful in a wide range of assays, separations, and purifications. In addition, dinuclear metal complexes of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes that are not so conjugated are used in the detection of phosphate esters of biological species by either MALDI-TOF mass spectrometry or by dye displacement.

Abstract translation: 将1,3-双（1,4,7-三氮杂环壬-1-烯）-2-羟基丙烷与多种共轭成员的共轭物用于形成与磷酸酯结合的双核金属络​​合物。 由于它们的共轭形式，复合物被并入到色谱介质，亲和力结合试剂和染料中，这使复合物可用于广泛的测定，分离和纯化。 此外，不结合的1,3-双（1,4,7-三氮杂环壬烷-1-基）-2-羟基丙烷的双核金属络​​合物用于通过MALDI-TOF检测生物物种的磷酸酯 质谱或染料置换。

公开(公告)号：US20140057293A1

公开(公告)日：2014-02-27

申请号：US13973596

申请日：2013-08-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Thomas R. Berkelman ,  Lisandra Martin ,  Rhiannon Jones ,  John Walker, II

IPC: G01N33/543 ,  C08F220/60

CPC classification number: G01N27/44747 ,  B01D15/3823 ,  C07D255/02 ,  C07F3/06 ,  C07F13/005 ,  C08F220/56 ,  C08F220/60 ,  G01N33/54306 ,  Y10T436/143333 ,  Y10T436/163333

Abstract: Conjugates of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes with a variety of conjugating members are used in the formation of dinuclear metal complexes which bind to phosphate esters. By virtue of their conjugated forms, the complexes are incorporated into chromatographic media, affinity binding reagents, and dyes, which make the complexes useful in a wide range of assays, separations, and purifications. In addition, dinuclear metal complexes of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes that are not so conjugated are used in the detection of phosphate esters of biological species by either MALDI-TOF mass spectrometry or by dye displacement.

Abstract translation: 将1,3-双（1,4,7-三氮杂环壬-1-烯）-2-羟基丙烷与多种共轭成员的共轭物用于形成与磷酸酯结合的双核金属络​​合物。 由于它们的共轭形式，复合物被并入到色谱介质，亲和力结合试剂和染料中，这使复合物可用于广泛的测定，分离和纯化。 此外，不结合的1,3-双（1,4,7-三氮杂环壬烷-1-基）-2-羟基丙烷的双核金属络​​合物用于通过MALDI-TOF检测生物物种的磷酸酯 质谱或染料置换。

公开(公告)号：US20170067852A1

公开(公告)日：2017-03-09

申请号：US15272257

申请日：2016-09-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Craig Rowell ,  Cory Panattoni ,  Thomas R. Berkelman ,  Sean Cater ,  Shane Petersen ,  Lee Olech ,  Xuemei Yang

IPC: G01N27/447 ,  C08F220/56 ,  B01D57/02

CPC classification number: G01N27/44747 ,  B01D57/02 ,  C08F220/56 ,  C08F222/385

Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

Abstract translation: 聚丙烯酰胺凝胶在蛋白质分离中提供高分辨率并且相对于水解而言比常规聚丙烯酰胺凝胶更稳定，其依赖于Tris或Tris-Bis作为缓冲剂，通过掺入三乙醇胺代替大部分或全部的Tris或Tris-Bis制备。

公开(公告)号：US09486741B2

公开(公告)日：2016-11-08

申请号：US13934936

申请日：2013-07-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Craig Rowell ,  Cory Panattoni ,  Thomas R. Berkelman ,  Sean Cater ,  Shane Petersen ,  Lee Olech ,  Xuemei Yang

IPC: G01N27/447 ,  B01D57/02 ,  C08F220/56

CPC classification number: G01N27/44747 ,  B01D57/02 ,  C08F220/56 ,  C08F222/385

Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

公开(公告)号：US09274103B2

公开(公告)日：2016-03-01

申请号：US13973596

申请日：2013-08-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Thomas R. Berkelman ,  Lisandra Martin ,  Rhiannon Jones ,  John Walker, II

IPC: C07D255/02 ,  G01N33/543 ,  C08F220/60 ,  B01D15/38

CPC classification number: G01N27/44747 ,  B01D15/3823 ,  C07D255/02 ,  C07F3/06 ,  C07F13/005 ,  C08F220/56 ,  C08F220/60 ,  G01N33/54306 ,  Y10T436/143333 ,  Y10T436/163333

Abstract: Conjugates of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes with a variety of conjugating members are used in the formation of dinuclear metal complexes which bind to phosphate esters. By virtue of their conjugated forms, the complexes are incorporated into chromatographic media, affinity binding reagents, and dyes, which make the complexes useful in a wide range of assays, separations, and purifications. In addition, dinuclear metal complexes of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes that are not so conjugated are used in the detection of phosphate esters of biological species by either MALDI-TOF mass spectrometry or by dye displacement.