公开(公告)号：US20150226703A1

公开(公告)日：2015-08-13

申请号：US14577824

申请日：2014-12-19

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Shane Petersen ,  Cory Panattoni ,  Craig Rowell

IPC: G01N27/447 ,  C07K1/26

CPC classification number: G01N27/44747 ,  C07K1/26 ,  Y10S436/826

Abstract: Polyacrylamide gels that offer high resolution as electrophoretic media for protein separations and an improved resistance to hydrolysis upon storage are made by including either taurine, asparagine, or both as an ampholyte, in combination with either tris(hydroxymethyl)-aminomethane or bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane as a buffer, plus other conventional components.

Abstract translation: 提供高分辨率的聚丙烯酰胺凝胶作为蛋白质分离的电泳介质，并且通过包含牛磺酸，天冬酰胺或两者作为两性电解质与三（羟甲基） - 氨基甲烷或双（2- 羟乙基）氨基 - 三（羟甲基）甲烷作为缓冲液，加上其他常规组分。

公开(公告)号：US10119934B2

公开(公告)日：2018-11-06

申请号：US15272257

申请日：2016-09-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Craig Rowell ,  Cory Panattoni ,  Thomas R. Berkelman ,  Sean Cater ,  Shane Petersen ,  Lee Olech ,  Xuemei Yang

IPC: G01N27/447 ,  B01D57/02 ,  C08F220/56

Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

公开(公告)号：US09486741B2

公开(公告)日：2016-11-08

申请号：US13934936

申请日：2013-07-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Craig Rowell ,  Cory Panattoni ,  Thomas R. Berkelman ,  Sean Cater ,  Shane Petersen ,  Lee Olech ,  Xuemei Yang

IPC: G01N27/447 ,  B01D57/02 ,  C08F220/56

CPC classification number: G01N27/44747 ,  B01D57/02 ,  C08F220/56 ,  C08F222/385

Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

公开(公告)号：US20170067852A1

公开(公告)日：2017-03-09

申请号：US15272257

申请日：2016-09-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Craig Rowell ,  Cory Panattoni ,  Thomas R. Berkelman ,  Sean Cater ,  Shane Petersen ,  Lee Olech ,  Xuemei Yang

IPC: G01N27/447 ,  C08F220/56 ,  B01D57/02

CPC classification number: G01N27/44747 ,  B01D57/02 ,  C08F220/56 ,  C08F222/385

Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

Abstract translation: 聚丙烯酰胺凝胶在蛋白质分离中提供高分辨率并且相对于水解而言比常规聚丙烯酰胺凝胶更稳定，其依赖于Tris或Tris-Bis作为缓冲剂，通过掺入三乙醇胺代替大部分或全部的Tris或Tris-Bis制备。