公开(公告)号：US20060226374A1

公开(公告)日：2006-10-12

申请号：US10567427

申请日：2004-08-06

申请人： Rudolf Rigler ,  Theo Lasser ,  Pierre-Andre Besse ,  Alexis Rochas ,  Alexandre Serov ,  Michael Gosch ,  Radivoje Popovic

发明人： Rudolf Rigler ,  Theo Lasser ,  Pierre-Andre Besse ,  Alexis Rochas ,  Alexandre Serov ,  Michael Gosch ,  Radivoje Popovic

IPC分类号： G01J1/58

CPC分类号： G01J3/2803 ,  G01J3/4406 ,  G01N21/645 ,  G01N21/6458 ,  G01N2021/6417 ,  G01N2021/6463 ,  G02B21/0024

摘要： The invention relates to a method and device for identifying luminescent molecules according to the fluorescence correlation spectroscopy method. The inventive method comprising the following steps: a) preparing a sample (12) containing luminescent molecules; b) illuminating the sample (12) using an optical excitation device (2,4,6,8) comprising at least one light source, at least one, in particular, diffractive optical element (7) for splitting light passing therethrough into multiple beams, and a focusing optics (8) for focusing multiple light beams passing therethrough into multiple confocal volume elements: c) capturing emission radiation from the multiple confocal volume elements by means of a locally resolving sensor matrix arrangement (20), whereby the sensor matrix arrangement is a sensor matrix, which is comprised of avalanche photodiodes AD is produced using IC technology, particularly CMOS technology, and is integrated in a sensor chip (20) with Greiger-mode wiring, and; d) processing the signals, which are provided by the avalanche photodiode matrix, by means of a signal processing and evaluation device preferably integrated in the sensor chip.

摘要翻译： 本发明涉及根据荧光相关光谱法鉴定发光分子的方法和装置。 本发明的方法包括以下步骤：a）制备含有发光分子的样品（12） b）使用包括至少一个光源的光学激发装置（2,4,6,8）照亮样品（12），至少一个特别是衍射光学元件（7），用于将穿过其中的光分成多个光束 以及用于将通过其中的多个光束聚焦成多个共焦体积元件的聚焦光学器件（8）：c）通过局部分辨传感器矩阵布置（20）捕获来自多个共焦体积元件的发射辐射，由此传感器矩阵布置 是由雪崩光电二极管组成的传感器矩阵，AD采用IC技术，特别是CMOS技术制造，并集成在具有Greiger模式布线的传感器芯片（20）中; d）通过优选地集成在传感器芯片中的信号处理和评估装置处理由雪崩光电二极管矩阵提供的信号。

公开(公告)号：US08480579B2

公开(公告)日：2013-07-09

申请号：US11912224

申请日：2006-04-20

申请人： Alexandre Serov ,  Theo Lasser

发明人： Alexandre Serov ,  Theo Lasser

IPC分类号： A61B5/05

CPC分类号： A61B5/0059 ,  A61B5/0261 ,  A61B5/413

摘要： A high-speed laser perfusion imaging instrument including a laser source, a detector, a signal-processing unit, data memory, and a screen to display results. A section of a sample surface is illuminated with laser light; reemitted light from the irradiated surface is collected by focusing optics on a 2D array of integrating photodetectors having elements that can be accessed individually or in a pre-defined selection of pixels at high speed. This 2D array measures intensity variations at each individual pixel. Average amplitude and mean frequency of the measured signal contain information about concentration and speed of moving blood cells. For real-time imaging, exposure time is used as a parameter to measure relative perfusion changes. These data are stored and processed with the signal-processing unit to deliver 2D flow maps of the illuminated sample section, and allow a simple overlay between a conventional image and processed flow maps.

摘要翻译： 一种高速激光灌注成像仪器，包括激光源，检测器，信号处理单元，数据存储器和显示结果的屏幕。 用激光照射样品表面的一部分; 通过将光学器件聚焦在具有可以单独访问的元素的集成光电检测器的2D阵列上或以高速度的预定义的像素选择来收集来自被照射的表面的光。 该2D阵列测量各个像素处的强度变化。 测量信号的平均振幅和平均频率包含关于移动血细胞的浓度和速度的信息。 对于实时成像，暴露时间用作测量相对灌注变化的参数。 这些数据与信号处理单元一起存储和处理，以提供照明样本部分的2D流程图，并允许传统图像和处理流程图之间的简单覆盖。

公开(公告)号：US20090118623A1

公开(公告)日：2009-05-07

申请号：US11912224

申请日：2006-04-20

申请人： Alexandre Serov ,  Theo Lasser

发明人： Alexandre Serov ,  Theo Lasser

IPC分类号： A61B6/00 ,  A61B5/02

CPC分类号： A61B5/0059 ,  A61B5/0261 ,  A61B5/413

摘要： A high-speed laser perfusion imaging instrument including a laser source, a detector, a signal-processing unit, data memory, and a screen to display results. A section of a sample surface is illuminated with laser light; reemitted light from the irradiated surface is collected by focusing optics on a 2D array of integrating photodetectors having elements that can be accessed individually or in a pre-defined selection of pixels at high speed. This 2D array measures intensity variations at each individual pixel. Average amplitude and mean frequency of the measured signal contain information about concentration and speed of moving blood cells. For real-time imaging, exposure time is used as a parameter to measure relative perfusion changes. These data are stored and processed with the signal-processing unit to deliver 2D flow maps of the illuminated sample section, and allow a simple overlay between a conventional image and processed flow maps.

摘要翻译： 一种高速激光灌注成像仪器，包括激光源，检测器，信号处理单元，数据存储器和显示结果的屏幕。 用激光照射样品表面的一部分; 通过将光学器件聚焦在具有可以单独访问的元素的集成光电检测器的2D阵列上或以高速度的预定义的像素选择来收集来自被照射的表面的光。 该2D阵列测量各个像素处的强度变化。 测量信号的平均振幅和平均频率包含关于移动血细胞的浓度和速度的信息。 对于实时成像，暴露时间用作测量相对灌注变化的参数。 这些数据与信号处理单元一起存储和处理，以提供照明样本部分的2D流程图，并允许传统图像和处理流程图之间的简单覆盖。