- Patent Title: Genome-wide construction of Schizosaccharomyces pombe heterozygous deletion mutants containing gene-specific barcodes by the methods of 4-round serial or block PCR, or total gene synthesis thereof
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Application No.: US12989192Application Date: 2008-08-27
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Publication No.: US09670480B2Publication Date: 2017-06-06
- Inventor: Kwang Lae Hoe , Dong Uk Kim , Mi Sun Won , Hyang Sook Yoo , Dong Sup Kim , Han Oh Park , Kyung Sook Chung , Young Joo Jang , Mi Young Nam , Sang Jo Han , Shin Jung Choi , Seung Tae Baek , Hyong Bai Kim , Kyung Sun Heo , Hye Mi Lee , Min Ho Lee , Jo Young Park
- Applicant: Kwang Lae Hoe , Dong Uk Kim , Mi Sun Won , Hyang Sook Yoo , Dong Sup Kim , Han Oh Park , Kyung Sook Chung , Young Joo Jang , Mi Young Nam , Sang Jo Han , Shin Jung Choi , Seung Tae Baek , Hyong Bai Kim , Kyung Sun Heo , Hye Mi Lee , Min Ho Lee , Jo Young Park
- Applicant Address: KR Daejeon
- Assignee: Korea Research Institute of Bioscience and Biotechnology
- Current Assignee: Korea Research Institute of Bioscience and Biotechnology
- Current Assignee Address: KR Daejeon
- Agency: Rubin and Rudman LLP
- Agent Judith U. Kim
- Priority: KR10-2008-0037420 20080422
- International Application: PCT/KR2008/005031 WO 20080827
- International Announcement: WO2009/131279 WO 20091029
- Main IPC: C40B50/00
- IPC: C40B50/00 ; C40B40/02 ; C40B30/06 ; C12N1/00 ; C12N15/10 ; C40B30/00
Abstract:
A method comprising transforming Schizosaccharomyces pombe with a deletion cassette, constructed by four-round serial PCR, block PCR or total gene synthesis, containing a homologous recombination site is provided for preparing gene-targeted heterozygous deletion Schizosaccharomyces pombe. Also provided are gene-targeted hetero2ygous deletion Schizosaccharomyces pombe mutants prepared by the method, and a library of gene-targeted heterozygous deletion Schizosaccharomyces pombe mutants. Further, the library is useful in constructing a method and a kit for screening a drug's modes of action.
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