发明授权
US5610036A Mutant AOX2 promoter, microorganism carrying same, method of preparation
thereof and production of heterologous protein using such microorganism
失效
突变体AOX2启动子，携带其的微生物，其制备方法和使用这种微生物产生异源蛋白质
- 专利标题： Mutant AOX2 promoter, microorganism carrying same, method of preparation thereof and production of heterologous protein using such microorganism
- 专利标题（中）： 突变体AOX2启动子，携带其的微生物，其制备方法和使用这种微生物产生异源蛋白质
-
申请号： US288899申请日： 1994-08-10
-
公开(公告)号： US5610036A公开(公告)日： 1997-03-11
- 发明人: Masami Miura , Yutaka Ishida , Hideyuki Oi , Koji Murakami , Yukimitsu Nakagawa , Haruhide Kawabe
- 申请人： Masami Miura , Yutaka Ishida , Hideyuki Oi , Koji Murakami , Yukimitsu Nakagawa , Haruhide Kawabe
- 申请人地址： JPX Osaka
- 专利权人： The Green Cross Corporation
- 当前专利权人： The Green Cross Corporation
- 当前专利权人地址： JPX Osaka
- 优先权： JPX3-63598 19910327; JPX3-63599 19910327
- 主分类号： C07K14/765
- IPC分类号： C07K14/765 ; C12N9/26 ; C12N15/14 ; C12N15/81 ; C07H21/04 ; C12N1/16 ; C12N1/19 ; C12P21/02
摘要：
A mutant AOX2 (alcohol oxidase 2) promoter derived from the natural AOX2 promoter by base sequence deletion, insertion, or substitution is disclosed. A microbial strain carrying a gene under the control of such a mutant AOX2 promoter can be obtained by growing in a methanol-containing medium a strain incapable of producing AOX encoded by the AOX1 gene but carrying the AOX2 gene under the control of the natural AOX2 promoter. A heterologous protein may be produced by cultivating the mutant strain with the desired heterologous protein gene incorporated downstream from the mutant AOX2 promoter. A plasmid carrying the mutant AOX2 promoter is also provided.
公开/授权文献
- US4924857A Surgical retractor 公开/授权日：1990-05-15
信息查询
