Disclosed are confocal microscope systems capable of spectral multiplexing. The systems beneficially provide multiple excitation spots on a sample plane simultaneously, and each spot can provide a different wavelength of excitation light. Scanning the excitation spots across a sample can therefore provide multispectral fluorescence imaging at a faster rate than through the sequential process of conventional laser scanning confocal microscopes. A method of generating a distribution from multiplexed spectral fluorescence data is also disclosed.