Embodiments of the present disclosure relate to a preparation and feed apparatus of a standard sample for calibration of a trace-analysis instrument, and especially to a preparation and feed apparatus of a standard sample for calibration of a gas chromatograph-ion mobility spectrometer. When the trace-analysis instrument is being calibrated by taking advantage of the preparation and feed apparatus according to embodiments of the disclosure, it is unnecessary to use an additional dedicated tool and steps to prepare the sample for testing and to use an organic solvent or a dedicated sample application/dispensing tool, resulting in that the trace-analysis instrument is simple and convenient to carry and use, and the substance for calibration is also convenient to store and exchange; moreover, the trace-analysis instrument is also safe, reliable and environmentally friendly.