The invention discloses a treating method of a palladium-on-carbon catalyst carrier for meropenem synthesis. The treating method comprises the steps that backflow treatment is carried out on an aqueous solution of a nitrogen-containing organic reagent for powdered activated carbon under microwave heating conditions, and after washing is carried out until the pH is 7.0-8.0, drying is carried out toobtain the palladium-on-carbon catalyst carrier for meropenem synthesis. The invention further discloses a preparing method of a palladium-on-carbon catalyst. The powdered activated carbon is subjected to backflowing treatment under the microwave heating conditions, uniform microwave heating is carried out, the uniformity of the reaction between a reagent and the surface of the activated carbon during the surface treatment of the activated carbon is ensured, the microwave heating efficiency is high, and the efficiency of carrier treatment is ensured; and the powdered activated carbon is subjected to backflowing treatment by adopting an aqueous solution of a nitrogen-containing organic reagent, alkaline groups are introduced on the surface of the activated carbon, the formation of four-membered ring open rings and dipolymer in the meropenem reaction is effectively inhibited by created alkaline environment and the prepared catalyst, the synthesis operation of meropenem is simplified, and the residual problem of organic reagents is solved.