The invention provides a kadsura coccinea acetic acid beverage as well as a preparation method and application thereof and relates to the technical field of foods. The kadsura coccinea acetic acid beverage comprises 5-10% of an acetic acid fermentation broth of a kadsura coccinea root ethanol extract, 10-20% of a lactic acid bacterium fermentation broth of kadsura coccinea fruits, 6-12% of auxiliary materials and the balance of water, wherein the lactic acid bacteria comprise lactobacillus plantarum Lact.chili.6 and lactobacillus plantarum Lact.chili.8. The kadsura coccinea acetic acid beverage provided by the invention is rich in antioxidant and anti-inflammatory function components such as polyphenols, lignans, triterpene substances and anthocyanins, has a DPPH* (1,1-diphenyl-2-picrylhydrazyl radical) clearance rate up to 90% or greater, has an inhibition activity IC50 value of 4.5g/L upon tyrosinase, has antibacterial diameters of 10mm or greater upon escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, tritirachium album, propionibacterium, diphtheroid bacilli and the like, and has effects such as face beautifying, sterilization and antivirus and aging prevention.