The invention provides a novel low-permeability rubber and resin combined fuel pipeline and a preparation method thereof. In the fuel pipeline provided by the invention, an F-TPV material layer, or aTHV material layer or an FEP material layer is used as an isolating layer which can achieve good fuel permeation resistance and chemical resistance, so the fuel pipeline provided by the invention canhave good fuel permeation resistance. Meanwhile, the pipeline structure and each material layer are regulated and optimized to realize that the fuel pipeline provided by the invention can satisfy therequirement on ultralow permeability (high resistance), and the performances of the fuel pipeline can satisfy the requirements of an original equipment manufacturer.