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发明公开
CN107653275A D-苯丙氨酸的制备方法 无效 - 驳回

D-苯丙氨酸的制备方法
摘要：
本发明公开了一种D-苯丙氨酸的制备方法，其中，所述制备方法包括：将反式肉桂酸、苯丙氨酸脱氨酶、L-氨基酸氧化酶、氨水和氨硼烷混合反应，制得D-苯丙氨酸。通过上述技术方案，本发明以反式肉桂酸作为底物原料，通过苯丙氨酸脱氨酶催化反式肉桂酸加氨(氨化)，生成L-苯丙氨酸和D-苯丙氨酸，反应体系中L-苯丙氨酸在L-氨基酸氧化酶的氧化作用下生成亚氨酸，亚氨酸在还原剂氨硼烷(NH3BH3)存在的条件下还原成L-苯丙氨酸和D-苯丙氨酸，从而使得反应过程产生的L-苯丙氨酸不断转化成D-苯丙氨酸。通过不断的循环转化，进而实现D-苯丙氨酸产率的大幅度提高，产率达到96％，D-苯丙氨酸的光学纯度超过99％。
摘要（英）：

The invention discloses a preparation method of D-phenylalanine. The preparation method comprises the steps of mixing trans-cinnamic acid, phenylalanine deaminase, L-amino-acid oxidase, ammonia waterand ammonia borane and performing reaction to prepare the D-phenylalanine. Through the adoption of the technical scheme, the trans-cinnamic acid serves as a substrate raw material, ammonification of the trans-cinnamic acid is catalyzed by the phenylalanine deaminase, L-phenylalanine and D-phenylalanine are generated, the L-phenylalanine in the reaction system is under the oxidation action of the L-amino-acid oxidase to generate imidic acid, and the imidic acid is reduced into the L-phenylalanine and the D-phenylalanine under the existence of a reducing agent ammonia borane (NH3BH3), so that the L-phenylalanine generated in the reaction process is continuously converted into the D-phenylalanine. Through continuous circulating conversion, the yield of the D-phenylalanine is greatly increased, the yield reaches to 96 percent, and the optical purity of the D-phenylalanine exceeds 99 percent.

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