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发明公开
CN105462868A 一种提高丙酮酸产量和生产强度的方法 有权

一种提高丙酮酸产量和生产强度的方法
摘要：
本发明公开了一种提高丙酮酸产量和生产强度的方法，属于发酵工程技术领域。本发明通过在光滑球拟酵母菌株中过表达线粒体丙酮酸载体蛋白，加强丙酮酸进入线粒体，进一步加强菌体的生长，从而提高生长偶联型产物丙酮酸的产量和生产强度，通过基因工程技术，将来源于酿酒酵母的MPC1基因过了表达于TgU-中，获得了一株高产丙酮酸的工程菌株TgU-(pY26-MPC1)，相对于对照菌株TgU-(pY26)，工程菌TgU-(pY26-MPC1)的细胞干重、葡萄糖消耗速率、丙酮酸的产量和丙酮酸生产强度相对于对照菌(TgU-(pY26))分别提高了24.9％、28.2％、91.9％和94.1％。
摘要（英）：

The invention discloses a method for improving the yield and production intensity of pyruvic acid, and belongs to the technical field of fermentation engineering. According to the method, pyruvic acid carrier proteins of mitochondria are over-expressed in a torulopsis glabrata strain, and enhanced pyruvic acid enters the mitochondria to further enhance the growth of the torulopsis glabrata, so that the yield and production intensity of a growth-related type product, namely, pyruvic acid are improved; MPC1 genes from saccharomyces cerevisiae are over-expressed in TgU- through a genetic engineering technology to obtain an engineering strain TgU-(pY26-MPC1) highly producing pyruvic acid; and compared with a reference strain TgU-(pY26), the dry cell weight, glucose consumption rate, pyruvic acid yield and pyruvic acid production intensity of the engineering strain TgU-(pY26-MPC1) are improved by 24.9%, 28.2%, 91.9% and 94.1% respectively.

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