知搜-全球专利检索

  1. 登录
  2. 注册
发明授权
CN104762277B 糖基化改造提高脂肪酶表达的方法、突变酶及其应用 有权 转让

糖基化改造提高脂肪酶表达的方法、突变酶及其应用
摘要：
本发明公开了糖基化改造提高脂肪酶表达的方法、突变酶及其应用，属于酶工程领域。本发明是将米根霉脂肪酶的前导肽序列进行N‑糖基化突变，将SAS和/或NT氨基酸分别改造为N‑糖基化位点NGT和/或NLT，得到的突变体酶proROLA、proROLB、proROLAB的胞外蛋白浓度分别比未进行糖基化的proROL提高了211％、188％、233％，发酵罐培养时酶活分别达到8210U·mL‑1、8457U·mL‑1和9366U·mL‑1，而未突变的proROL的胞外酶活几乎为零。本发明的米根霉脂肪酶具有显著提高的脂肪酶酶活，可以应用于食品、化工及生物能源等领域。
摘要（英）：

Provided are a method for improving the expression of lipase by glycosylation modification, mutant enzymes and an application of the mutant enzymes, specifically comprising: performing N-glycosylation mutation on a propeptide sequence of rhizopus oryzaelipase, and respectively modifying SAS and/or NT amino acids into N-glycosylation loci NGT and/or NLT; compared with the extracelluar protein concentration of proROL which is not subjected to glycosylation, extracelluar protein concentrations of obtained mutant enzymes proROLA, proROLB and proROLAB are separately improved by 211 percent, 188 percent and 233 percent; and when a fermentation tank is adopted for culturing, enzyme activities separately reach 8210U·mL-1, 8457U·mL-1 and 9366U·mL-1, and the extracellular enzyme activity of unmutated proROL is almost zero.

公开/授权文献
0/0