The invention belongs to the technical field of plant immunity detection and particularly relates to hybridoma cell strains capable of secreting monoclonal antibodies resisting fumonisins B1 as well as the preparation and application of recombinant monoclonal antibodies H7 of the monoclonal antibodies. The fumonisins B1 are conjugated with the carrier proteins of KLH and BSA respectively to obtain antigens; the obtained antigens FB1-KLH are used for immunizing mice; after cell fusion, selective culture is performed on the immunized mice; hybridoma cells are screened through the FB1-BSA conjugates; the hybridoma cell strains 2C5 which can stably secret the monoclonal antibodies resisting the fumonisins B1 are obtained by subcloning. The recombinant monoclonal antibodies H7 of the hybridoma cell strains 2C5 are screened according to the antibody gene pool technology and the phage display technology. According to the invention, a large quantity of the monoclonal antibodies and the recombinant monoclonal antibodies H7 are produced and purified. The obtained monoclonal antibodies 2C5 and the recombinant monoclonal antibodies H7 can be used for the immunological detection of the fumonisins B1.