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发明公开
CN103233004A 一种人工DNA分子及检测目标基因表达的方法 失效 - 权利终止

一种人工DNA分子及检测目标基因表达的方法
摘要：
本发明提供一种DNA分子，所述DNA分子的碱基序列为SEQ ID NO.1或SEQ ID NO.2、SEQ ID NO.3或SEQ ID NO.4所示，其能够编码一种TALE-TFs，所述TALE-TFs能够识别SEQ ID NO5中相应位点。本发明利用TALE-TFs技术使不表达β-酪蛋白基因的哺乳动物成纤维细胞能够表达β-酪蛋白基因，也就实现了用TALE-TFs在成纤维细胞中激活重组的β-酪蛋白基因——外源整合基因表达框，为体外检测重组的β-酪蛋白启动子驱动的表达框提供一种更加方便的检测途径，无需培养乳腺上皮细胞。
摘要（英）：

The invention provides a DNA (deoxyribonucleic acid) molecule. The base sequence of the DNA molecule is shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 or SEQ ID NO.4, and can code a TALE-TFs; and the TALE-TFs can identify corresponding loci in SEQ ID NO.5. According to the invention, a mammal fibroblast cell which does not express a beta-casein gene can express the beta-casein gene through the TALE-TFs technology, thus realizing the activation of a recombinant beta-casein gene (exogenous integrator gene expression cassette) in the fibroblast cell through TALE-TFs, and providing a more convenient detection way for the in-vitro detection of an expression cassette driven by a recombinant beta-casein promoter without culturing a mammary gland epithelial cell.

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