The invention relates to a malaria vaccine and a preparation method thereof and belongs to the technical field of medicaments. A recombinant silkworm baculovirus is constructed, the 19kD segment (MSP119C) at a tail end of a plasmodium falciparum merozoite surface membrane protein 1C is expressed on the surface of the silkworm baculovirus by baculovirus surface display technology, a recombinant baculovirus is generated by using a silkworm pupa as a biological reactor, and the malaria vaccine is prepared from the recombinant baculovirus. Compared with the traditional malaria vaccine production method, the method has the characteristics of high safety, low production cost, high yield, easiness in operation and suitability for mass production.