In order to solve problems of high production cost, complex production technology and low production efficiency of present starch-lipid complexes as well as the difficulty of realizing large industrial production, the invention provides a preparation method of a starch-lipid complex. The preparation method provided by the invention comprises the following steps of: 1, preparing an emulsion; 2, adding starch into the emulsion for mixing; 3, performing pasting of the starch; 4, cooling the starch; 5, carrying out high pressure homogenization on the mixture by the application of a high pressure homogenizer; 6, slowly cooling the starch-lipid mixture after high pressure homogenization; 7, carrying out vacuum drying on the cooled product and crushing to obtain the starch-lipid complex. The starch-lipid complex prepared by the invention can be used to effectively improve the texture and structural stability of starch-based materials, delay the aging of starch, and simultaneously enhance the hydrolyzing resistance of starch to amylase. The preparation method of the starch-lipid complex has advantages of simple technology, high production efficiency and low cost, and is suitable for industrial production.