The invention discloses a method and a kit for identifying the early embryonic sex of a pig by dual temperature multiplex PCR. A dual temperature multiplex PCR reaction system with rapidness, sensitiveness and high accuracy, which is suitable for identifying the early embryonic sex of the pig, is used for identifying the early embryonic sex of the pig. The kit which is developed by the dual temperature multiplex PCR reaction system and used for identifying the early embryonic sex of the pig fully plays the characteristics of high efficiency, economy, simpleness, convenience and high reaction specificity of the dual temperature multiplex PCR and realizes the rapid identification of the early embryo of the pig, thereby realizing the sex control of the pig, improving the economic benefits ofpig breeding industry, saving the breeding cost and providing convenience.