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发明授权
CN101029314B 玉米淀粉分支酶基因siRNA表达载体 失效 - 权利终止

玉米淀粉分支酶基因siRNA表达载体
摘要：
本发明涉及一种玉米淀粉分支酶基因siRNA表达载体，属于植物基因工程和遗传育种领域。用表达载体pCAMBIA1301上的多克隆位点，将玉米淀粉分支酶种子特异启动子基因插入Sph I、Sal I两个酶切位点之间，玉米淀粉分支酶基因SBEIIa反义片段插入上述启动子下游Sal I、Sac I之间，在切除潮霉素抗生素抗性基因后，玉米淀粉分支酶基因SBE IIa的正义片段被插入到Sac I、Xho I之间，在Bgl II和BstE II两个酶切位点之间插入绿色荧光蛋白基因GFP。本发明创造的优点是通过转基因技术高效封闭玉米淀粉分支酶基因sbe IIa的表达，降低玉米淀粉分支酶的活性，提高玉米直链淀粉含量，培育高直链淀粉玉米或创造新的高直链玉米淀粉种质资源。
摘要（英）：

A corn starch branching enzyme gene siRNA expression carrier is prepared by taking multiple clone site on expression pCAMBIA1301, inserting corn starch branching enzyme seed specific promoter gene between SphI and SalI, inserting SBEIIa antisense fragment between SalI and SacI, cutting hygromix antibiotic resistant gene, inserting SBEIIa positive-sense fragment between SacI and XhoI and insertinggreen fluorescent protein gene GFP between BglII and BstEII. It can enclose corn starch branching enzyme gene sbeIIa expression, decrease its activity, improve corn straight-chain starch corn yield and cultivate high-straight-chain starch corn. resources.

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