公开(公告)号：US20230072639A1

公开(公告)日：2023-03-09

申请号：US17759267

申请日：2021-01-22

申请人： GLYCOM A/S

发明人： Margit PEDERSEN ,  Isotta D'ARRIGO ,  Katrine Bych KAMPMANN ,  Manos PAPADAKIS

IPC分类号： C12N5/10 ,  C12P19/00 ,  C12N15/74

摘要： The present invention relates to the field of recombinant production of biological molecules in genetically modified cells. More particularly, it relates to a method for recombinant production of human milk oligosaccharides (HMO) using a genetically modified cell expressing a protein of the major facilitator superfamily (MFS), the protein expressed being Bad.

公开(公告)号：US20220241349A1

公开(公告)日：2022-08-04

申请号：US17724386

申请日：2022-04-19

申请人： Korea University Research and Business Foundation

发明人： Kyoung Heon KIM ,  Yong-Su Jin ,  Eun-Ju Yun ,  Dong-Hyun Kim ,  Sora Yu ,  Kyung-mun Cho ,  Na Jeong Park

IPC分类号： A61K35/74 ,  A23L33/125 ,  A23L33/135 ,  A23L33/00 ,  A61K35/745 ,  A61K47/26 ,  C12P19/00

摘要： The present invention relates to a method for producing marine algae-derived agarotriose, and a use thereof as a prebiotic. More specifically, the present invention investigates the characteristics of agarotriose as a prebiotic which is selectively metabolized by probiotic microorganisms, thereby enabling agarotriose to be used as an anti-cancer or anti-inflammatory agent in the fields of food and pharmaceuticals, and enabling agarotriose to be obtained at high yield through efficient purification with minimal loss after enzymatic hydrolysis of a red algae-derived polysaccharide without pre-treatment.

公开(公告)号：US11337999B2

公开(公告)日：2022-05-24

申请号：US16764742

申请日：2018-11-15

申请人： Korea University Research and Business Foundation

发明人： Kyoung Heon Kim ,  Yong-Su Jin ,  Eun-Ju Yun ,  Dong-Hyun Kim ,  Sora Yu ,  Kyung-mun Cho ,  Na Jeong Park

IPC分类号： A61K35/741 ,  A61K35/74 ,  A23L33/125 ,  A23L33/135 ,  A23L33/00 ,  A61K35/745 ,  A61K47/26 ,  C12P19/00 ,  A61K35/00

摘要： The present invention relates to a method for producing marine algae-derived agarotriose, and a use thereof as a prebiotic. More specifically, the present invention investigates the characteristics of agarotriose as a prebiotic which is selectively metabolized by probiotic microorganisms, thereby enabling agarotriose to be used as an anti-cancer or anti-inflammatory agent in the fields of food and pharmaceuticals, and enabling agarotriose to be obtained at high yield through efficient purification with minimal loss after enzymatic hydrolysis of a red algae-derived polysaccharide without pre-treatment.

公开(公告)号：US20220090030A1

公开(公告)日：2022-03-24

申请号：US17382462

申请日：2021-07-22

申请人： DUPONT NUTRITION BIOSCIENCES APS

发明人： Slavko Kralj

IPC分类号： C12N9/10 ,  A23L33/10 ,  A23L2/52 ,  C12P19/00

摘要： An isolated and/or purified α-glucanotransferase from Exiguobacterium acetylicum, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the α-glucanotransferase and variants thereof, host cells comprising the synthetic nucleic acids, and compositions comprising the α-glucanotransferase are provided. Methods of using the compositions include the manufacture of oligosaccharides.

公开(公告)号：US20220056497A1

公开(公告)日：2022-02-24

申请号：US17354819

申请日：2021-06-22

申请人： Glycosyn LLC

发明人： John M. McCoy ,  Matthew Ian Heidtman ,  Massimo Merighi

IPC分类号： C12P19/18 ,  C12N9/10 ,  C12P19/00 ,  C07H13/04 ,  C12N15/70 ,  C12P19/04

摘要： The invention provides compositions and methods for engineering E. coli or other host production bacterial strains to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.

公开(公告)号：US20210371890A1

公开(公告)日：2021-12-02

申请号：US17326442

申请日：2021-05-21

申请人： UPM-KYMMENE CORPORATION

发明人： Juha Tamper ,  Sami Turunen

IPC分类号： C12P19/14 ,  C12P19/02 ,  C12M1/40 ,  C12P19/00 ,  C13K1/02

摘要： In a method and an apparatus for an enzymatic hydrolysis in which plant based raw material is hydrolysed by means of enzymes in at least one enzymatic hydrolysis stage. A plant based feed (1) is fed to the enzymatic hydrolysis stage (2) in which the plant based feed is hydrolysed. A liquid fraction (3) comprising carbohydrates is separated from a solid fraction (4) in a solid-liquid separation stage (11). At least a part (5) of the solid fraction (4) comprising enzymes is recirculated to the plant based feed (1) of the enzymatic hydrolysis stage (2) or to the enzymatic hydrolysis stage (2), and a rest part (6) of the solid fraction (4) is recovered. Further, the invention relates to the liquid fraction and the solid fraction and their use.

公开(公告)号：US11046984B2

公开(公告)日：2021-06-29

申请号：US15307914

申请日：2015-05-14

申请人： Glycosyn LLC

发明人： John M. McCoy ,  Matthew Ian Heidtman ,  Massimo Merighi

IPC分类号： C12N1/21 ,  C12P19/18 ,  C12P19/28 ,  C12N9/12 ,  C12N9/10 ,  C12P19/00 ,  C07H13/04 ,  C12N15/70 ,  C12P19/04

摘要： The invention provides compositions and methods for engineering E. coli or other host production bacterial strains to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.

公开(公告)号：US11028419B2

公开(公告)日：2021-06-08

申请号：US15442127

申请日：2017-02-24

申请人： Glycosyn LLC

发明人： Massimo Merighi ,  John M. McCoy ,  Matthew Ian Heidtman

IPC分类号： C12P19/18 ,  C12P19/00 ,  C12N9/00 ,  C12N9/10 ,  C12N9/38 ,  C12P19/26 ,  C07H3/06 ,  C07H13/04 ,  C12N15/70 ,  C12N9/14

摘要： The invention provides compositions and methods for engineering bacteria to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.

公开(公告)号：US10982246B2

公开(公告)日：2021-04-20

申请号：US16166215

申请日：2018-10-22

申请人： Centro de Ingenierla Genética y Biotecnologia

发明人： Enrique Rosendo Pérez Cruz ,  Lázaro Hernández Garcia ,  Duniesky Martínez García ,  Luis Enrique Trujillo Toledo ,  Carmen Menéndez Rodríguez ,  Alina Sobrino Legón ,  Ricardo Ramírez Ibañez ,  Gumersindo Feijoo Costa ,  Juan Manuel Lema Rodicio

IPC分类号： C12P19/00 ,  A23L5/00 ,  A23L29/30 ,  A23L27/30 ,  A23L33/10 ,  A23L2/60 ,  C12P19/18 ,  C07H3/00 ,  C07H3/02 ,  A23K20/163 ,  C07H3/06 ,  C12N9/10

摘要： The present invention discloses an industrial scale method to obtain 1-kestose by the use of a recombinant fructosyltransferase (FTF), isolated from Festuca arundinacea, expressed constitutively in a non-saccharolytic yeast. In this invention, the recombinant FTF type sucrose:sucrose 1-fructosyltransferase (1-SSTrec) is produced constitutively, stable and at high yield, both in the culture supernatant and in intact cells of the host Pichia pastoris. Hence, the invention additionally provides a method for 1-SST production at industrial scale. The recombinant enzyme is then used for mass production of short-chain fructooligosaccharides (FOS), specifically 1-kestose, from sucrose. The method of the present invention establishes conditions that allow conversion rates where the synthesized FOS constitute above 55% (w/w) of the total sugars in the reaction mixture and the 1-kestose content reaches values higher than 90% (w/w) of the total FOS fraction.

公开(公告)号：US10808271B2

公开(公告)日：2020-10-20

申请号：US16305272

申请日：2017-05-31

申请人： NESTEC S.A.

发明人： Lubbert Dijkhuizen ,  Joana Gangoiti Munecas ,  Sander Sebastiaan Van Leeuwen ,  Christina Vafeiadi ,  Stephane Duboux

IPC分类号： C12P19/18 ,  C12N9/26 ,  C12N9/44 ,  C12P19/00 ,  A23L33/21 ,  A23K20/189 ,  C12P19/04

摘要： The present invention relates to the field of poly- and oligosaccharides and their dietary effects. In particular it relates to a method of producing an α-glucan containing (α1→3) linked D-glucose units. The invention also provides a branched α-glucan comprising alternating (α1→4) and (α1→3) glucosidic linkages and having (α1→3,4) branching points, a food composition, and the use of an α-glucanotransferase enzyme for reducing the digestible carbohydrates of a starch containing food material. Further aspects of the invention are a bacteria, an enzyme and an expression vector.