公开(公告)号：US4148876A

公开(公告)日：1979-04-10

申请号：US882445

申请日：1978-03-01

Applicant: June D. Almeida ,  David C. Edwards

Inventor： June D. Almeida ,  David C. Edwards

IPC: A61K9/127 ,  A61K39/145 ,  A61K39/39 ,  A61K39/12 ,  A61K39/18 ,  A61K39/20 ,  A61K39/22

CPC classification number: A61K39/145 ,  A61K39/12 ,  A61K39/39 ,  A61K9/1271 ,  A61K2039/55555 ,  C12N2760/16134 ,  Y10S424/812

Abstract: A novel antigenic preparation which comprises a plurality of microvesicles are unilamellar bodies comprising a single lipid bilayer upon the exterior surface of which is bound an antigenic protein derived from a virus and having a hydrophobic region.

Abstract translation: 包含多个微泡的新型抗原制剂是包含单一脂质双层的单层体，其外表面与衍生自病毒的抗原蛋白结合并具有疏水区。

公开(公告)号：US4826687A

公开(公告)日：1989-05-02

申请号：US867539

申请日：1986-05-28

Applicant: Kuniaki Nerome ,  Akira Oya ,  Kunio Ohkuma ,  Atsuo Inoue

Inventor： Kuniaki Nerome ,  Akira Oya ,  Kunio Ohkuma ,  Atsuo Inoue

IPC: A61K39/102 ,  A61K9/127 ,  A61K9/50 ,  A61K39/145 ,  A61K39/39 ,  A61P31/12 ,  A61P31/16 ,  A61K39/22 ,  A61K39/12 ,  B01J13/02

CPC classification number: A61K39/145 ,  A61K39/12 ,  A61K9/1271 ,  A61K9/1272 ,  A61K9/1277 ,  A61K9/5068 ,  A61K2039/5555 ,  A61K2039/55555 ,  C12N2760/16134 ,  Y10S436/829 ,  Y10S514/885 ,  Y10T428/2984

Abstract: This invention relates to a novel influenza vaccine comprising a complex of HANA antigen and an MDP derivative.The novel vaccine is prepared by mixing an influenza HANA antigen and at least one MDP derivative in a suitable medium; solubilizing the resulting mixture with a surface active agent capable of being removed by dialysis, the solubilization being conducted in the presence or absence of cholesterol, lecithin and dicetyl phosphate or a mixture thereof; and then removing the surface active agent therefrom by dialysis to obtain an influenza vaccine comprising artificial vesicle-like particles of a complex of HANA antigen and MDP derivative, where the MDP derivative forms a membrane of the particle (corresponding to the lipid membrane of natural influenza virus particle) on the surface of which there exists the HANA antigen being bonded to the MDP derivative so as to form the complex. Thus, said artificial vesicle-like particle of the HANA antigen-MDP derivative complex has nearly the same particle size and shape as the natural influenza virus particle.The novel vaccine has an improved immunogenicity compared with the conventional vaccine.

Abstract translation: 本发明涉及包含HANA抗原和MDP衍生物的复合物的新型流感疫苗。 通过将流感HANA抗原和至少一种MDP衍生物混合在合适的培养基中来制备新型疫苗; 将所得混合物与能够通过透析除去的表面活性剂溶解，所述溶解在胆固醇，卵磷脂和磷酸二鲸蜡酯或其混合物存在或不存在下进行; 然后通过透析除去表面活性剂，得到包含HANA抗原和MDP衍生物的复合物的人造囊泡状颗粒的流感疫苗，其中MDP衍生物形成颗粒膜（对应于天然流感的脂质膜 病毒颗粒），其表面上存在与MDP衍生物键合的HANA抗原，以形成复合物。 因此，HANA抗原-MDP衍生物复合物的所述人造囊泡状颗粒具有与天然流感病毒颗粒几乎相同的粒度和形状。 与常规疫苗相比，新型疫苗具有改善的免疫原性。