公开(公告)号：US20170096695A1

公开(公告)日：2017-04-06

申请号：US15311850

申请日：2015-04-27

申请人： Evonik Degussa GmbH

发明人： OLIVER THUM ,  PHILIP ENGEL ,  CHRISTIAN GEHRING ,  STEFFEN SCHAFFER ,  MIRJA WESSEL

IPC分类号： C12P19/44 ,  C12N9/10 ,  C12N15/52 ,  C12N9/14

CPC分类号： C12P19/44 ,  A01N25/30 ,  C12N9/0004 ,  C12N9/1051 ,  C12N9/14 ,  C12N15/52 ,  C12Y204/01159

摘要： The present invention relates to a method of preparing at least one rhamnolipid comprising: contacting a recombinant cell with a medium containing a carbon source; and culturing the cell under suitable conditions for preparation of the rhamnolipid from the carbon source by the cell, wherein the recombinant cell has been genetically modified such that, compared to the wild-type of the cell, the cell has an increased activity of at least one of the enzymes E1, E2 and E3, wherein the enzyme E1 is an α/β hydrolase, the enzyme E2 is a rhamnosyltransferase I and the enzyme E3 is a rhamnosyl-transferase II, and wherein the carbon source is a C4 molecule.

公开(公告)号：US20190233856A1

公开(公告)日：2019-08-01

申请号：US16334781

申请日：2017-10-18

申请人： Evonik Degussa GmbH

发明人： Oliver Thum ,  Steffen Schaffer ,  Christoph Schorsch ,  Mirja Wessel

IPC分类号： C12P7/64 ,  C12P19/44 ,  C12N9/10 ,  C12N9/02 ,  C07K14/21 ,  C12R1/39 ,  C12R1/40 ,  C12R1/385

CPC分类号： C12P7/6463 ,  C07K14/195 ,  C07K14/21 ,  C12N9/0036 ,  C12N9/1029 ,  C12P19/44 ,  C12R1/385 ,  C12R1/39 ,  C12R1/40 ,  C12Y101/05002 ,  C12Y204/01159 ,  C12Y207/01 ,  C12Y207/03009

摘要： The invention relates to cells which make rhamnolipids and are genetically modified such that they have a decreased activity, compared to the wild type thereof, of an ABC glucose transporter and, compared to the wild type thereof, an increased activity of at least one non-ABC glucose transporter and to a method for producing rhamnolipids using the cells according to the invention.