公开(公告)号：US20150106963A1

公开(公告)日：2015-04-16

申请号：US14512797

申请日：2014-10-13

申请人： Genus, PLC ,  E.J. Squires, Ltd.

发明人： E. James Squires

IPC分类号： C12N9/02 ,  C12Q1/68 ,  A01K67/027

CPC分类号： C12N9/0085 ,  A01K67/0275 ,  A01K2227/108 ,  C12N9/004 ,  C12N15/8509 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Y106/02002 ,  C12Y401/0203

摘要： Novel mutations in cytochrome P450C17 (CYP17) and cytochrome b5 (CYB5) affecting 16-androstene steroid synthesis are disclosed. The novel mutations result in alterations in production of critical intermediaries in the synthesis of 16-androstene steroids. Altering the activity of these enzymes may be useful in enhancing reducing androstenone synthesis and reducing boar taint. The identification of these novel mutations also allows for the development of transgenic pigs bearing mutations in these enzymes or for genetic screening to identify pigs on the basis of their CYP17 and/or CYB5 genotype. Pigs having these mutations may be selected and bred to produce pigs that have a lower incidence of boar taint.

摘要翻译： 公开了影响16-雄烯类固醇合成的细胞色素P450C17（CYP17）和细胞色素b5（CYB5）的新突变。 新型突变导致关键中间体在合成16-雄烯甾类固醇中的生产变化。 改变这些酶的活性可用于增强还原和雄烯酮合成和减少野猪污点。 这些新突变的鉴定还允许开发在这些酶中具有突变的转基因猪或用于遗传筛选以基于其CYP17和/或CYB5基因型鉴定猪的转基因猪。 可以选择并育种具有这些突变的猪，以产生具有较低的野猪污染发生率的猪。

公开(公告)号：US06448055B1

公开(公告)日：2002-09-10

申请号：US09380262

申请日：1999-08-27

申请人： Sakayu Shimizu ,  Michihiko Kobayashi

发明人： Sakayu Shimizu ,  Michihiko Kobayashi

IPC分类号： C12N902

CPC分类号： C12Y106/02002 ,  C12N9/0083 ,  C12P7/6427 ,  C12P7/6472 ,  C12Y114/19001

摘要： Genomic DNA and cDNA encoding &Dgr;9-desaturase from a microorganism belonging to the subgenus Mortierella of the genus Mortierella, an expression vector for expression thereof, and a transformant are disclosed. A method for producing &Dgr;9-desaturase by use of a gene encoding the enzyme is also disclosed. Introduction of the &Dgr;9-desaturase gene of the present invention into an unsaturated fatty acid producing cell can enhance conversion into palmitoleic acid or oleic acid, starting materials for unsaturated fatty acids, and can increase the production of unsaturated fatty acids. By combining a gene for cytochrome b5 or a gene for cytochrome b5 reductase, constituents of the microsomal electron transport system, with the &Dgr;9-desaturase of the present invention, more efficient production can be expected. &Dgr;9-desaturase can also be produced with high efficiency by a recombinant DNA technology.

摘要翻译： 公开了来自属于被孢霉属的被孢霉属亚属的微生物，其表达的表达载体和转化体的编码DELTA9-去饱和酶的基因组DNA和cDNA。 还公开了通过使用编码该酶的基因产生DELTA9-去饱和酶的方法。 将本发明的DELTA9-去饱和酶基因导入不饱和脂肪酸生产细胞可以增加转化为棕榈油酸或油酸，不饱和脂肪酸的起始原料，并且可以增加不饱和脂肪酸的产生。 通过将细胞色素b5的基因或细胞色素b5还原酶的基因，微粒体电子传递系统的组分与本发明的DELTA9-去饱和酶组合，可以预期更有效的生产。 也可以通过重组DNA技术以高效率生产DELTA9-去饱和酶。

公开(公告)号：US20130276157A1

公开(公告)日：2013-10-17

申请号：US13790678

申请日：2013-03-08

申请人： Genus, PLC ,  E. J. SQUIRES, LTD.

发明人： E. James Squires

IPC分类号： C12N15/85

CPC分类号： C12N9/0085 ,  A01K67/0275 ,  A01K2227/108 ,  C12N9/004 ,  C12N15/8509 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Y106/02002 ,  C12Y401/0203

摘要： Novel mutations in cytochrome P450C17 (CYP17) and cytochrome b5 (CYB5) affecting 16-androstene steroid synthesis are disclosed. The novel mutations result in alterations in production of critical intermediaries in the synthesis of 16-androstene steroids. Altering the activity of these enzymes may be useful in enhancing reducing androstenone synthesis and reducing boar taint. The identification of these novel mutations also allows for the development of transgenic pigs bearing mutations in these enzymes or for genetic screening to identify pigs on the basis of their CYP17 and/or CYB5 genotype. Pigs having these mutations may be selected and bred to produce pigs that have a lower incidence of boar taint.

公开(公告)号：US20160235821A1

公开(公告)日：2016-08-18

申请号：US15087563

申请日：2016-03-31

申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

发明人： Mi Sun WON ,  Hyun Seung BAN ,  Bo Kyung KIM ,  Soon Woo NAM ,  Kyeong LEE ,  Young-Ju LEE ,  Hong-Sub LEE ,  Dong Uk KIM ,  Ki Cheol PARK ,  Joo-Young IM ,  Kyoung-Jin OH

IPC分类号： A61K38/44 ,  G01N33/574 ,  C12N7/00

CPC分类号： A61K38/44 ,  A61K48/005 ,  C12N7/00 ,  C12N9/004 ,  C12N2710/10043 ,  C12N2710/10071 ,  C12Y106/02002 ,  G01N33/57492 ,  G01N2333/90209

摘要： The present invention relates to a pharmaceutical composition for treating cancer, containing cytochrome b5 reductase 3 (CYB5R3) protein as an active ingredient. Over-expression of CYB5R3 in cancer cells results in significant reduction of hypoxia-inducible factor-1α (HIF-1α) expression, which leads inhibition of cancer cells growth in vitro and in vivo. Thus, the CYB5R3 gene or protein of the present invention can be useful as an active ingredient of a pharmaceutical composition for treating cancer.

摘要翻译： 本发明涉及用于治疗癌症的药物组合物，其含有细胞色素b5还原酶3（CYB5R3）蛋白作为活性成分。 癌细胞中CYB5R3的过度表达导致缺氧诱导因子-1α（HIF-1α）表达的显着降低，这导致体外和体内癌细胞生长的抑制。 因此，本发明的CYB5R3基因或蛋白质可以用作治疗癌症的药物组合物的活性成分。

公开(公告)号：US20050031605A1

公开(公告)日：2005-02-10

申请号：US10772076

申请日：2004-02-03

申请人： Howard Bunn ,  Jianxin Xie ,  Hao Zhu

发明人： Howard Bunn ,  Jianxin Xie ,  Hao Zhu

IPC分类号： A61K20060101 ,  A61K38/44 ,  A61K48/00 ,  A61P3/10 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/68

CPC分类号： G01N33/502 ,  A01K2217/05 ,  A01K2217/075 ,  A61K31/445 ,  A61K38/44 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Y106/02002 ,  G01N33/6893 ,  G01N2500/00 ,  G01N2800/042 ,  A61K2300/00

摘要： The invention features compositions and methods for increasing insulin production or decreasing blood glucose levels. Also included are methods of treating diabetes and obesity.

摘要翻译： 本发明的特征在于用于增加胰岛素产生或降低血糖水平的组合物和方法。 还包括治疗糖尿病和肥胖症的方法。