CYB5 AND CYP17 MUTATIONS FOR ALTERATION OF 16-ANDROSTENE STEROID SYNTHESIS AND REDUCED BOAR TAINT IN PIGS
    1.
    发明申请
    CYB5 AND CYP17 MUTATIONS FOR ALTERATION OF 16-ANDROSTENE STEROID SYNTHESIS AND REDUCED BOAR TAINT IN PIGS 审中-公开
    CYB5和CYP17突变体,用于改变16位安慰剂甾体合成和减少血小板

    公开(公告)号:US20150106963A1

    公开(公告)日:2015-04-16

    申请号:US14512797

    申请日:2014-10-13

    发明人: E. James Squires

    IPC分类号: C12N9/02 C12Q1/68 A01K67/027

    摘要: Novel mutations in cytochrome P450C17 (CYP17) and cytochrome b5 (CYB5) affecting 16-androstene steroid synthesis are disclosed. The novel mutations result in alterations in production of critical intermediaries in the synthesis of 16-androstene steroids. Altering the activity of these enzymes may be useful in enhancing reducing androstenone synthesis and reducing boar taint. The identification of these novel mutations also allows for the development of transgenic pigs bearing mutations in these enzymes or for genetic screening to identify pigs on the basis of their CYP17 and/or CYB5 genotype. Pigs having these mutations may be selected and bred to produce pigs that have a lower incidence of boar taint.

    摘要翻译: 公开了影响16-雄烯类固醇合成的细胞色素P450C17(CYP17)和细胞色素b5(CYB5)的新突变。 新型突变导致关键中间体在合成16-雄烯甾类固醇中的生产变化。 改变这些酶的活性可用于增强还原和雄烯酮合成和减少野猪污点。 这些新突变的鉴定还允许开发在这些酶中具有突变的转基因猪或用于遗传筛选以基于其CYP17和/或CYB5基因型鉴定猪的转基因猪。 可以选择并育种具有这些突变的猪,以产生具有较低的野猪污染发生率的猪。

    &Dgr;9-desaturase gene
    2.
    发明授权
    &Dgr;9-desaturase gene 有权
    DELTA9-去饱和酶基因

    公开(公告)号:US06448055B1

    公开(公告)日:2002-09-10

    申请号:US09380262

    申请日:1999-08-27

    IPC分类号: C12N902

    摘要: Genomic DNA and cDNA encoding &Dgr;9-desaturase from a microorganism belonging to the subgenus Mortierella of the genus Mortierella, an expression vector for expression thereof, and a transformant are disclosed. A method for producing &Dgr;9-desaturase by use of a gene encoding the enzyme is also disclosed. Introduction of the &Dgr;9-desaturase gene of the present invention into an unsaturated fatty acid producing cell can enhance conversion into palmitoleic acid or oleic acid, starting materials for unsaturated fatty acids, and can increase the production of unsaturated fatty acids. By combining a gene for cytochrome b5 or a gene for cytochrome b5 reductase, constituents of the microsomal electron transport system, with the &Dgr;9-desaturase of the present invention, more efficient production can be expected. &Dgr;9-desaturase can also be produced with high efficiency by a recombinant DNA technology.

    摘要翻译: 公开了来自属于被孢霉属的被孢霉属亚属的微生物,其表达的表达载体和转化体的编码DELTA9-去饱和酶的基因组DNA和cDNA。 还公开了通过使用编码该酶的基因产生DELTA9-去饱和酶的方法。 将本发明的DELTA9-去饱和酶基因导入不饱和脂肪酸生产细胞可以增加转化为棕榈油酸或油酸,不饱和脂肪酸的起始原料,并且可以增加不饱和脂肪酸的产生。 通过将细胞色素b5的基因或细胞色素b5还原酶的基因,微粒体电子传递系统的组分与本发明的DELTA9-去饱和酶组合,可以预期更有效的生产。 也可以通过重组DNA技术以高效率生产DELTA9-去饱和酶。